Phospholipid Bilayer Softening Due to Hydrophobic Gold Nanoparticle Inclusions

Chakraborty, Saptarshi; Abbasi, Akram; Bothun, Geoffrey D.; Nagao, Michihiro; Kitchens, Christopher L.

doi:10.1021/acs.langmuir.8b02553

Cited by 21 publications

(18 citation statements)

References 84 publications

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“…Thus, these theoretical and experimental efforts have led to a unifying picture that enables the extraction of the main elastic and viscous parameters of the membrane through NSE measurements 77 . This type of neutron study has been used to investigate the effect of different components in the membrane, such as various lipids [78][79][80][81] , cholesterol 82 , drugs 83 , proteins [84][85][86] and nanoparticles 87,88 .…”

Section: [H1] Key Aspects Of Neutron Scatteringmentioning

confidence: 99%

High-resolution neutron spectroscopy using backscattering and neutron spin-echo spectrometers in soft and hard condensed matter

et al. 2020

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Section: [H1] Key Aspects Of Neutron Scatteringmentioning

confidence: 99%

High-resolution neutron spectroscopy using backscattering and neutron spin-echo spectrometers in soft and hard condensed matter

et al. 2020

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“…Other applications of NSE spectroscopy include studies involving changes in membrane bending rigidity in response to additive molecules, such as trehalose (Brüning et al, 2014), melittin (Sharma et al, 2015;Sharma and Qian, 2019), and inorganic nanoparticles used in drug delivery (Chakraborty et al, 2018). These studies provide a nanoscale perspective of how small molecules could affect the softening or stiffening of lipid membranes under biologically relevant conditions and in targeted applications.…”

Section: Neutron Spin Echomentioning

confidence: 99%

Biomembrane Structure and Material Properties Studied With Neutron Scattering

et al. 2021

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Cell membranes and their associated structures are dynamical supramolecular structures where different physiological processes take place. Detailed knowledge of their static and dynamic structures is therefore needed, to better understand membrane biology. The structure–function relationship is a basic tenet in biology and has been pursued using a range of different experimental approaches. In this review, we will discuss one approach, namely the use of neutron scattering techniques as applied, primarily, to model membrane systems composed of lipid bilayers. An advantage of neutron scattering, compared to other scattering techniques, is the differential sensitivity of neutrons to isotopes of hydrogen and, as a result, the relative ease of altering sample contrast by substituting protium for deuterium. This property makes neutrons an ideal probe for the study of hydrogen-rich materials, such as biomembranes. In this review article, we describe isotopic labeling studies of model and viable membranes, and discuss novel applications of neutron contrast variation in order to gain unique insights into the structure, dynamics, and molecular interactions of biological membranes. We specifically focus on how small-angle neutron scattering data is modeled using different contrast data and molecular dynamics simulations. We also briefly discuss neutron reflectometry and present a few recent advances that have taken place in neutron spin echo spectroscopy studies and the unique membrane mechanical data that can be derived from them, primarily due to new models used to fit the data.

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“…It has been found that the incorporation of hydrophilic gold nanoparticles at the liposomal lipid bilayer results in membrane softening relative to pure liposomes, manifested by reducing bending modulus. The membrane softening phenomena is both size and concentration dependent [35]. Some investigations include perturbation of lipid properties upon nanoparticle adsorption, based on atomistic simulations.…”

Section: Effect Of Nanoparticles On Liposomal Transition Temperaturementioning

confidence: 99%

Pulse Magnetic Fields Induced Drug Release from Gold Coated Magnetic Nanoparticle Decorated Liposomes

Acharya

Chikán

2020

Magnetochemistry

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Magnetic nanoparticle-assisted drug release from liposomes is an important way to enhance the functionality/usefulness of liposomes. This work demonstrates an approach how to integrate magnetic nanoparticles with liposomes with the assistance of gold–thiol chemistry. The gold coated magnetic particles cover the thiolated liposomes from the outside, which removes the competition of the drug molecules and the triggering magnetic particles to free the inner space of the liposomes when compared to previous magneto liposome formulations. The liposome consists of dipalmitoyl phosphatidylcholine (DPPC) combined with distearoylphosphatidylcholine (DSPC) in addition to regular cholesterol or cholesterol-PEG-SH. Permeability assays and electron microscopy images show efficient coupling between the liposomes and nanoparticles in the presence of thiol groups without compromising the functionality of the liposomes. The nanoparticles such as gold nanoparticles, gold coated iron oxide nanoparticles and bare iron oxide nanoparticles are added following the model drug encapsulation. The efficient coupling between the gold coated nanoparticles (NPs) and the thiolate liposomes is evidenced by the shift in transition temperature of the thiolated liposomes. The addition of magnetically triggerable nanoparticles externally makes the entire interior of liposomes available for drug loading. The drug release efficiencies of these liposomes/NPs complexes were compared under exposure to pulsed magnetic fields. The results indicate up to 20% of the drug can be released in short time, which is comparable in efficiency to previous studies performed when magnetic NPs were located inside liposomes. Interestingly, the liposomes were found to exhibit variations in release efficiency based on different dilution media which is attributed to an osmotic pressure effect on liposomal stability.

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Phospholipid Bilayer Softening Due to Hydrophobic Gold Nanoparticle Inclusions

Cited by 21 publications

References 84 publications

High-resolution neutron spectroscopy using backscattering and neutron spin-echo spectrometers in soft and hard condensed matter

High-resolution neutron spectroscopy using backscattering and neutron spin-echo spectrometers in soft and hard condensed matter

Biomembrane Structure and Material Properties Studied With Neutron Scattering

Pulse Magnetic Fields Induced Drug Release from Gold Coated Magnetic Nanoparticle Decorated Liposomes

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