Phosphoproteomics: Detection, Identification and Importance of Protein Phosphorylation

Jia, Min; Lin, Kah Wai; Souchelnytskyi, Serhiy

doi:10.5772/31305

Cited by 3 publications

(2 citation statements)

References 67 publications

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“…Recently, protein phosphorylation has also emerged as an important process in biological adhesives. Among the four types of phosphorylation currently described ( O -phosphorylation, N -phosphorylation, S -phosphorylation and acylphosphorylation) [ 136 , 137 ], only O -phosphorylation of serine residues has been detected in some marine adhesive proteins [ 87 , 120 ]. So far phosphoproteins have been reported among mussel foot proteins (Mefp-5 [ 29 ], Mgfp-5 [ 138 ], Mcfp-5 and Mcfp-6 [ 26 ]), and tubeworm cement proteins (Pc-3A and Pc-B [ 34 ]).…”

Section: Molecular Tools To Characterize Protein-based Adhesivesmentioning

confidence: 99%

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Experimental strategies for the identification and characterization of adhesive proteins in animals: a review

et al. 2015

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Adhesive secretions occur in both aquatic and terrestrial animals, in which they perform diverse functions. Biological adhesives can therefore be remarkably complex and involve a large range of components with different functions and interactions. However, being mainly protein based, biological adhesives can be characterized by classical molecular methods. This review compiles experimental strategies that were successfully used to identify, characterize and obtain the full-length sequence of adhesive proteins from nine biological models: echinoderms, barnacles, tubeworms, mussels, sticklebacks, slugs, velvet worms, spiders and ticks. A brief description and practical examples are given for a variety of tools used to study adhesive molecules at different levels from genes to secreted proteins. In most studies, proteins, extracted from secreted materials or from adhesive organs, are analysed for the presence of post-translational modifications and submitted to peptide sequencing. The peptide sequences are then used directly for a BLAST search in genomic or transcriptomic databases, or to design degenerate primers to perform RT-PCR, both allowing the recovery of the sequence of the cDNA coding for the investigated protein. These sequences can then be used for functional validation and recombinant production. In recent years, the dual proteomic and transcriptomic approach has emerged as the best way leading to the identification of novel adhesive proteins and retrieval of their complete sequences.

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Section: Molecular Tools To Characterize Protein-based Adhesivesmentioning

confidence: 99%

“…Different analytical methods have been developed for the detection and quantification of O -phosphorylation [ 136 , 137 ]. Below are some examples.…”

Section: Molecular Tools To Characterize Protein-based Adhesivesmentioning

confidence: 99%

Experimental strategies for the identification and characterization of adhesive proteins in animals: a review

et al. 2015

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Molecular cloning, expression, mRNA secondary structure and immunological characterization of mussel foot proteins (Mfps) (Mollusca: Bivalvia)

Anand

Vardhanan

2023

Journal of Biomolecular Structure and Dynamics

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Divide and conquer: subproteomic approaches toward gastric cancer biomarker and drug target discovery

Lin

Tan

Guan

et al. 2014

Expert Review of Proteomics

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The discovery of biomarkers for early detection and treatment for gastric cancer are two important gaps that proteomics have the potential to fill. Advancements in mass spectrometry, sample preparation and separation strategies are crucial to proteomics-based discoveries and subsequent translations from bench to bedside. A great number of studies exploiting various subproteomic approaches have emerged for higher-resolution analysis (compared with shotgun proteomics) that permit interrogation of different post-translational and subcellular compartmentalized forms of the same proteins as determinants of disease phenotypes. This is a unique and key strength of proteomics over genomics. In this review, the salient features, competitive edges and pitfalls of various subproteomic approaches are discussed. We also highlight valuable insights from several subproteomic studies that have increased our understanding of the molecular etiology of gastric cancer and the findings that led to the discovery of potential biomarkers/drug targets that were otherwise not revealed by conventional shotgun expression proteomics.

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Phosphoproteomics: Detection, Identification and Importance of Protein Phosphorylation

Cited by 3 publications

References 67 publications

Experimental strategies for the identification and characterization of adhesive proteins in animals: a review

Experimental strategies for the identification and characterization of adhesive proteins in animals: a review

Molecular cloning, expression, mRNA secondary structure and immunological characterization of mussel foot proteins (Mfps) (Mollusca: Bivalvia)

Divide and conquer: subproteomic approaches toward gastric cancer biomarker and drug target discovery

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