Phosphorylated ERK5/BMK1 transiently accumulates within division spindles in mouse oocytes and preimplantation embryos

Maciejewska, Zuzanna; Pascal, Aude; Kubiak, Jacek Z.; Ciemerych, Maria A.

doi:10.5603/fhc.2011.0074

Cited by 6 publications

References 52 publications

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Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos

Kubiak

2012

Methods in Molecular Biology

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Protein phosphorylation plays a pivotal role in cell cycle regulation. MPF (M-phase Promoting Factor) and MAPK (Mitogen-activated protein kinase) are two major kinases driving oocyte maturation and early embryonic divisions. Their activities can be measured experimentally with kinase assays that use specific exogenous substrates. The activities of MPF and MAPK are measured using histone H1 kinase and MBP (Myelin Basic Protein) kinase assays, respectively. Here, we describe detailed procedures for measuring these two activities in mouse and rat oocytes and in early mouse embryos. The assays we describe can be performed using very small amounts of biological material and produce clearly discernible measurements of histone H1 and MBP kinase activities.

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Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos

Kubiak

2012

Methods in Molecular Biology

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Polarity and Asymmetry During Mouse Oogenesis and Oocyte Maturation

Kloc

Ghobrial

Borsuk

et al. 2012

Results and Problems in Cell Differentiation

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Cell polarity and asymmetry play a fundamental role in embryo development. The unequal segregation of determinants, cues, and activities is the major event in the differentiation of cell fate and function in all multicellular organisms. In oocytes, polarity and asymmetry in the distribution of different molecules are prerequisites for the progression and proper outcome of embryonic development. The mouse oocyte, like the oocytes of other mammals, seems to apply a less stringent strategy of polarization than other vertebrates. The mouse embryo undergoes a regulative type of development, which permits the full rectification of development even if the embryo loses up to half of its cells or its size is experimentally doubled during the early stages of embryogenesis. Such pliability is strongly related to the proper oocyte polarization before fertilization. Thus, the molecular mechanisms leading to the development and maintenance of oocyte polarity must be included in any fundamental understanding of the principles of embryo development. In this chapter, we provide an overview of current knowledge regarding the development and maintenance of polarity and asymmetry in the distribution of organelles and molecules in the mouse oocyte. Curiously, the mouse oocyte becomes polarized at least twice during ontogenesis; the question of how this phenomenon is achieved and what role it might play is addressed in this chapter.

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ERK5 is essential for early porcine embryonic development by maintaining Endoplasmic Reticulum homeostasis

Lee,

et al. 2025

Gene

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Phosphorylated ERK5/BMK1 transiently accumulates within division spindles in mouse oocytes and preimplantation embryos

Cited by 6 publications

References 52 publications

Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos

Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos

Polarity and Asymmetry During Mouse Oogenesis and Oocyte Maturation

ERK5 is essential for early porcine embryonic development by maintaining Endoplasmic Reticulum homeostasis

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