Phosphorylation of a wheat aquaporin at two sites enhances both plant growth and defense

Lü, Kai; Chen, Xiaochen; Yao, Xiaohui; An, Yuyan; Wang, Xuan; Qin, Lu‐Ping; Li, Xiaoxu; Wang, Zuodong; Liu, Shuo; Sun, Zhimao; Zhang, Liyuan; Chen, Lei; Li, Baoyan; Liu, Baoyou; Wang, Weiyang; Ding, Xinhua; Yang, Yonghua; Zhang, Meixiang; Zou, Shenshen; Dong, Hansong

doi:10.1016/j.molp.2022.10.003

Cited by 32 publications

(35 citation statements)

References 75 publications

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“…The function of ROS, including superoxide anion, H 2 O 2 , and hydroxyl radicals, in the plant resistance to pathogen mainly consists of direct antimicrobial ability, lignification of the cell wall, induction of HR, production of PR proteins, generation of phytoalexins (like flavonoid), and other defense responses ( Shetty et al., 2008 ; Li et al., 2021b ; Feng et al., 2022 ). H 2 O 2 , acting as a signal, can activate downstream defense responses, and recent studies showed that H 2 O 2 signal could be transduced to aquaporins to endow a resistant ability against pest ( Lu et al., 2022 ) and disease ( Zhang et al., 2021 ) in plant. In this paper, the content of superoxide anion was significantly higher in Tc3d than that in other treatments, while the content of H 2 O 2 was the lowest together with the C3d treatment.…”

Section: Discussionmentioning

confidence: 99%

Arbuscular mycorrhizal fungus alleviates anthracnose disease in tea seedlings

Chen

Ye²,

Sun³

et al. 2023

Front. Plant Sci.

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Tea has been gaining increasing popularity all over the world in recent years, and its yield and quality depend on the growth and development of tea plants [Camellia sinensis (L.) Kuntze] in various environments. Nowadays, biotic stress and extreme weather, such as high temperature, drought, waterlogging, pests, and diseases, bring about much pressure on the production of tea with high quality. Wherein anthracnose, which is the most common and serious disease of tea plants, has earned more and more attention, as its control mainly relies on chemical pesticides. Arbuscular mycorrhizal fungi (AMF), forming symbiosis with most terrestrial plants, participate in plant resistance against the anthracnose disease, which was found by previous studies in a few herbaceous plants. However, there are a few studies about arbuscular mycorrhizal (AM) fungal regulation of the resistance to the anthracnose pathogen in woody plants so far. In this paper, we investigated the effect of AMF on the development of anthracnose caused by Colletotrichum camelliae and tried to decipher the pertinent mechanism through transcriptome analysis. Results showed that inoculating AMF significantly reduced the damage of anthracnose on tea seedlings by reducing the lesion area by 35.29% compared to that of the control. The content of superoxide anion and activities of catalase and peroxidase significantly increased (P < 0.05) in mycorrhizal treatment in response to the pathogen with 1.23, 2.00, and 1.39 times higher, respectively, than those in the control. Pathways of plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signaling, and phenylpropanoid biosynthesis might play roles in this regulation according to the transcriptomic results. Further redundancy analysis (RDA) and partial least squares structural equation modeling (PLS-SEM) analysis found that plant hormones, such as auxin and ethylene, and the antioxidant system (especially peroxidase) were of great importance in the AM fungal alleviation of anthracnose. Our results preliminarily indicated the mechanisms of enhanced resistance in mycorrhizal tea seedlings to the anthracnose pathogen and provided a theoretical foundation for the application of AMF as one of the biological control methods in tea plantations.

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Section: Discussionmentioning

confidence: 99%

Arbuscular mycorrhizal fungus alleviates anthracnose disease in tea seedlings

Chen

Ye²,

Sun³

et al. 2023

Front. Plant Sci.

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“…However, the P f of ZmPIP2;5N241K did not increase upon addition of COR (Figure 8D), suggesting that N241 of ZmPIP2;5 is crucial to its COR‐specific binding. Serine residue S280 of TaPIP2;10 and isoleucine residue I254 of HvPIP2;4 are crucial for transporting CO 2 into cells to enhance photosynthesis (Mori et al, 2014; Lu et al, 2022). Similarly, S121 of TaPIP2;10 is phosphorylated to transport H 2 O 2 into the cytoplasm for intensifying host defenses (Lu et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Serine residue S280 of TaPIP2;10 and isoleucine residue I254 of HvPIP2;4 are crucial for transporting CO 2 into cells to enhance photosynthesis (Mori et al, 2014; Lu et al, 2022). Similarly, S121 of TaPIP2;10 is phosphorylated to transport H 2 O 2 into the cytoplasm for intensifying host defenses (Lu et al, 2022). S280/S283 phosphorylation at the C terminus of PIP2;1 is enhanced by ethylene for water transport rate increases in Arabidopsis cells (Qing et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Coronatine promotes maize water uptake by directly binding to the aquaporin ZmPIP2;5 and enhancing its activity

Wang

et al. 2023

JIPB

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Water uptake is crucial for crop growth and development and drought stress tolerance. The water channel aquaporins (AQP) play important roles in plant water uptake. Here, we discovered that a jasmonic acid analog, coronatine (COR), enhanced maize (Zea mays) root water uptake capacity under artificial water deficiency conditions. COR treatment induced the expression of the AQP gene Plasma membrane intrinsic protein 2;5 (ZmPIP2;5). In vivo and in vitro experiments indicated that COR also directly acts on ZmPIP2;5 to improve water uptake in maize and Xenopus oocytes. The leaf water potential and hydraulic conductivity of roots growing under hyperosmotic conditions were higher in ZmPIP2;5‐overexpression lines and lower in the zmpip2;5 knockout mutant, compared to wild‐type plants. Based on a comparison between ZmPIP2;5 and other PIP2s, we predicted that COR may bind to the functional site in loop E of ZmPIP2;5. We confirmed this prediction by surface plasmon resonance technology and a microscale thermophoresis assay, and showed that deleting the binding motif greatly reduced COR binding. We identified the N241 residue as the COR‐specific binding site, which may activate the channel of the AQP tetramer and increase water transport activity, which may facilitate water uptake under hyperosmotic stress.

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“…The phosphorylation of the CsPIP2;1 protein at Ser273 is important for the regulation of water transport activity and water reabsorption (Jang et al., 2014). Phosphorylation at Ser280 or Ser121 makes TaPIP2;10 transport CO 2 or H 2 O 2 : both molecules induce stomatal closure, improving both crop productivity and immunity (Lu et al., 2022). These results indicate that PIP2;8 may be regulated by downstream peptides, hormones and receptor‐like protein kinases of PLATZ4.…”

Section: Discussionmentioning

confidence: 99%

Regulation of drought tolerance in Arabidopsis involves the PLATZ4‐mediated transcriptional repression of plasma membrane aquaporin PIP2;8

et al. 2023

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SUMMARY Plant A/T‐rich protein and zinc‐binding protein (PLATZ) transcription factors play important roles in plant growth, development and abiotic stress responses. However, how PLATZ influences plant drought tolerance remains poorly understood. The present study showed that PLATZ4 increased drought tolerance in Arabidopsis thaliana by causing stomatal closure. Transcriptional profiling analysis revealed that PLATZ4 affected the expression of a set of genes involved in water and ion transport, antioxidant metabolism, small peptides and abscisic acid (ABA) signaling. Among these genes, the direct binding of PLATZ4 to the A/T‐rich sequences in the plasma membrane intrinsic protein 2;8 (PIP2;8) promoter was identified. PIP2;8 consistently reduced drought tolerance in Arabidopsis through inhibiting stomatal closure. PIP2;8 was localized in the plasma membrane, exhibited water channel activity in Xenopus laevis oocytes and acted epistatically to PLATZ4 in regulating the drought stress response in Arabidopsis. PLATZ4 increased ABA sensitivity through upregulating the expression of ABSCISIC ACID INSENSITIVE 3 (ABI3), ABI4 and ABI5. The transcripts of PLATZ4 were induced to high levels in vegetative seedlings under drought and ABA treatments within 6 and 3 h, respectively. Collectively, these findings reveal that PLATZ4 positively influences plant drought tolerance through regulating the expression of PIP2;8 and genes involved in ABA signaling.

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Phosphorylation of a wheat aquaporin at two sites enhances both plant growth and defense

Cited by 32 publications

References 75 publications

Arbuscular mycorrhizal fungus alleviates anthracnose disease in tea seedlings

Arbuscular mycorrhizal fungus alleviates anthracnose disease in tea seedlings

Coronatine promotes maize water uptake by directly binding to the aquaporin ZmPIP2;5 and enhancing its activity

Regulation of drought tolerance in Arabidopsis involves the PLATZ4‐mediated transcriptional repression of plasma membrane aquaporin PIP2;8

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