Phosphoserine phosphatase as a prognostic biomarker in patients with gastric cancer and its potential association with immune cells

Abstract

Background Because of dismal prognosis in gastric cancer, identifying relevant prognostic factors is necessary. Phosphoserine phosphatase (PSPH) exhibits different expression patterns in many cancers and has been reported to affect the prognosis of patients with cancer. In this study, we examined the prognostic role of metabolic gene PSPH in gastric cancer based on the TCGA dataset and our hospital–based cohort cases. Methods We collected and analysed RNA-seq data of Pan-cancer and gastric cancer in the TCGA dataset and PSPH expression data obtained from immunohistochemical analysis of 243 patients with gastric cancer from Sun Yat-sen University cancer center. Further, Kaplan–Meier survival analysis and Cox analysis were used to assess the effect of PSPH on prognosis. The ESTIMATE and Cibersort algorithms were used to elucidate the relationship between PSPH and the abundance of immune cells using the TCGA dataset. Results We observed that PSPH expression displayed considerably high in gastric cancer and it was significantly associated with inferior prognosis (P = 0.043). Surprisingly, there was a significant relationship between lower immune scores and high expression of PSPH (P < 0.05). Furthermore, patients with a low amount of immune cells exhibited poor prognosis (P = 0.046). The expression of PSPH significantly increased in activated memory CD4 T cells, resting NK cells and M0 macrophages (P = 0.037, < 0.001, and 0.005, respectively). Conclusions This study highlighted that PSPH influences the prognosis of patients with gastric cancer, and this is associated with the infiltration of tumour immune cells, indicating that PSPH may be a new immune-related target for treating gastric cancer.

Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc × Guangdong small spotted pig

“…GO enrichment analysis results showed that DAPs enriched in each pathway are involved in various structures and functions such as oxidative phosphorylation, energy production, aerobic respiration, protein binding and transmembrane transport, antioxidant and various enzyme activities (34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46). We also found the GO terms that we are concerned about that are closely related to lipid metabolism and lipogenesis: taking LDM vs. BM as an example, 6 DAPs clustered on lipid binding belonging to molecular functions, 9 DAPs aggregate in lipid metabolism processes in biological processes, lipid transport and lipid regulation processes each gather 2 DAPs.…”

20-Hydroxyecdysone Confers Antioxidant and Antineoplastic Properties in Human Non-Small Cell Lung Cancer Cells

“…Our analysis of Kaplan–Meier plots derived from data on 1925 lung cancer patients clearly confirmed the strong oncogenic role of HK2, LDHA, PHGDH, PSAT1, SHMT2, MTHFD2, c-Myc, and ATF4 (CREB-2) in lung cancer. The high expression of genes coding for all of these enzymes and their transcriptional regulators is strongly associated with a shortened patient survival in different types of malignancies [ 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ].…”

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