Photo-Assisted Robust Anti-Interference Self-Powered Biosensing of MicroRNA Based on Pt–S Bonds and the Inorganic–Organic Hybridization Strategy

Abstract

Photo-assisted biofuel cell-based self-powered biosensors (PBFC-SPBs) possess the advantages of no need for external power supply, ease of sensing design, and simple instruments. In this work, a robust anti-interference PBFC-SPB for microRNA detection was constructed based on the Pt–S bond and the inorganic–organic hybridization strategy. The organic semiconductor [6,6]-phenyl-C61-butyric acid methylester@anthraquinone (PCBM@anthraquinone) served as an efficient light-harvesting material, and gold nanoparticle@Pt (AuNP@Pt) nanomaterials were immobilized on the surface via electrostatic adsorption for the binding of DNA. Notably, compared to Au–S bonds for DNA immobilization, the Pt–S bond exhibited better anti-interference ability. Ingeniously, cadmium sulfide quantum dots (CdS QDs) were close to the PCBM@anthraquinone substrate electrode to form sensitization structures, which was beneficial to enhance the photocurrent signal. Combining with the laccase-mimicking activity Cu2+/carbon nanotubes (Cu2+/CNTs) cathode, the PBFC-SPB for microRNA detection was achieved. Once the target existed, the identical sequence complementary microRNA would make DNA2/CdS dissociate and break away from the electrode, leading to a low signal. The linear detection range was 10 fM–100 pM, with the limit of determination of 2.4 fM (3S/N). The as-proposed strategy not only paves a new way for the design of photoelectrochemical biosensing but also opens a door for the construction of robust anti-interference bioassay for microRNA detection.

PEDOT/FeOOH/BiVO4 Nanohybrids with Excellent Photoelectric Performance Promoted by Photothermal Effects for the Ultrasensitive Detection of MicroRNA-375-3p

“…MicroRNAs (miRNAs) are involved in post-transcriptional repression of mRNA and found to be dysregulated in multiple cancer types. The abnormal miRNA concentration has been identified to be associated with many human diseases. , MiRNA-375-3p is prominently overexpressed in the blood of prostate cancer patients particularly and is related to the process of tumorigenesis and metastasis . Therefore, the development of accurate and sensitive detective methods is essential for the early diagnosis and monitoring of prostate cancer.…”

An all-graphdiyne electrochemiluminescence biosensor for the ultrasensitive detection of microRNA-21 based on target recycling with DNA cascade reaction for signal amplification

“…MiRNA-21 is an important marker for various tumors, 1 so the determination of miRNA-21 is significant for the preliminary diagnosis of cancer. The detection methods for miRNA-21 include microarray and quantitative real-time reverse transcription PCR, 2 Northern blotting, 3 electrochemistry, [4][5][6][7] photoelectrochemistry (PEC), [8][9][10] fluorescence, [11][12][13] and electrochemiluminescence (ECL). [14][15][16] Compared with the above detection methods, ECL has attracted considerable attention because of its greater linear range, lower detection limit, and superior sensitivity.…”

Light-Driven Self-Cascade Peroxidase-like Nanozymes without Exogenous H2O2

“…Photofuel cells (PFCs) are a kind of solar-triggered two-electrode device to realize the direct conversion of both solar energy and chemical fuel to electrical energy, which have been extensively used in self-powered sensors. , The construction of easy-to-functionalize PFC platforms plays an important role in its biosensing application, and the introduction of cascade photoassisted nanozymes will offer more possibility.…”