Photo-responsive tetraether lipids based vesicles for prophyrin mediated vascular targeting and direct phototherapy

Mahmoud, Gihan; Jedelská, Jarmila; Strehlow, Boris; Omar, S. M.; Schneider, Marc; Bakowsky, Udo

doi:10.1016/j.colsurfb.2017.08.049

Cited by 19 publications

(13 citation statements)

References 36 publications

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“…The results could be explained previously, where the massive accumulation of PpIX in the blood vessels and endothelial layers most particularly resulted in rearrangement of the cytoskeleton of endothelium, leading to damage of blood vessels accompanied eventually with thrombosis and micro vessel occlusion (Johansson & Andersson-Engels, 2010 ). A rapidly provoked thrombosis/hemorrhage and the collateral shutdown of the surrounding structures in- and outside the irradiated area were previously also reported in case of low mole fraction TELs based liposomes (Mahmoud et al., 2015 , 2017 ). However, as vascular shutdown lead to hypoxia in tumor regions thereby the oxygen depletion might hamper the process of PDT completion.…”

Section: Resultssupporting

confidence: 54%

“…This, in turn, indicates a strong fixation of the adjacent molecules of PpIX in the lipid mono/bilayer which most probably dissipates the energy migration among PpIX molecules, a phenomenon called self-quenching effect (Kachatkou et al., 2009 ; Reshetov et al., 2011 ; Huynh & Zheng, 2014 ). The gradual appearance of the emission band at 633–36 nm (peak of the monomer) with increasing TL: PpIX ratio from 10 to 100 and, in a similar study, from 50 to 500 confirmed PpIX monomerization as TELs incorporated in liposomes (Mahmoud et al., 2017 ).…”

Section: Resultssupporting

confidence: 53%

“…Unlike PDT studies in animal models, the irradiation of transparent CAM with an appropriate wavelength is feasible and the light tissue-penetration is not a key determined step. The potential uses of TELs were previously comprehensively evaluated as a platform for photodynamic therapy at our laboratory (Mahmoud et al., 2015 , 2017 ). In this study, TELs were assembled to form highly stabilized liposomes prevailing sustained release pattern.…”

Section: Introductionmentioning

confidence: 99%

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Stabilized tetraether lipids based particles guided prophyrins photodynamic therapy

et al. 2018

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Photodynamic therapy (PDT) that involves ergonomically delivered light in the presence of archetypical photosensitizer such as Protoporphyrin IX (PpIX) is a time-honored missile strategy in cancer therapeutics. Yet, the premature release of PpIX is one of the most abundant dilemma encounters the therapeutic outcomes of PDT due to associated toxicity and redistribution to serum proteins. In this study, ultrastable tetraether lipids (TELs) based liposomes were developed. PpIX molecules were identified to reside physically in the monolayer; thereby the inherent π-π stacking that leads to aggregation of PpIX in aqueous milieu was dramatically improved. TEL29.9 mol% and TEL62mol% based liposomes revealed PpIX sustained release diffusion pattern from spherical particles as confirmed by converged fitting to Baker & Lonsdale model. Stability in presence of human serum albumins, a key element for PDT accomplishment was emphasized. The epitome candidates were selected for vascular photodynamic (vPDT) in in-Ovo chick chorioallantoic membrane. Profoundly, TEL62mol% based liposomes proved to be the most effective liposomes that demonstrated localized effect within the irradiated area without eliciting quiescent vasculatures damages. Cellular photodynamic therapy (cPDT) revealed that various radiant exposure doses of 134, 202, 403 or 672 mJ.cm−2 could deliberately modulate the photo-responses of PpIX in TEL-liposomes.

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Section: Resultssupporting

confidence: 54%

Section: Resultssupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

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Stabilized tetraether lipids based particles guided prophyrins photodynamic therapy

et al. 2018

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“…The three mechanisms underlying PDT-mediated tumor destruction involve cell toxic reactive oxygen species (ROS) generation, tumor-associated vasculature damage, and immune response activation against the tumor. As a result, oxidation of cellular organelles can lead to apoptosis, necrosis, or autophagy of the cell [9,10]. The advantages of PDT include limited tissue damage, as illuminated light is restricted only to the photosensitized area with no major longterm systemic side effects [11].…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes

Ambreen

Duse

Tariq

et al. 2020

Cancers

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Photodynamic therapy (PDT) is a minimally invasive therapeutic approach used in the treatment of various medical conditions and cancerous diseases, involving light, a photosensitizing substance, and oxygen. Curcumin, a naturally occurring compound, carries antitumor activities and potentially could be exploited as a photosensitizer in PDT. Only little is known about liposomal-encapsulated curcumin that could help in increasing the efficacy, stability, and bioavailability of this compound. This study investigates the in vitro effects of curcumin-loaded liposomes in combination with PDT. Three papilloma virus-associated cell lines were treated with curcumin-loaded liposomes corresponding to a curcumin concentration of 0–100 µmol/L for 4 h followed by illumination at 457 nm (blue) for 45, 136, and 227 s at a fluence of 220.2 W/m2 (100 mA) corresponding to 1, 3 and 5 J·cm−2. After 24 h, the biological outcome of the treatment was assessed with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), SYTO9/PI (propidium iodide), Annexin V-FITC (fluorescein isothiocyanate)/PI, clonogenic survival, and scratch (wound closure) assays. Photoactivation of curcumin-loaded liposomes led to a significant reduction in colony formation and migratory abilities, as well as to an increase in tumor cell death. The results point to the combination of curcumin-loaded liposomes with PDT as a potentially useful tool for the treatment of papillomavirus-associated malignancies.

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“…Liposomes provide certain advantages as drug delivery vesicles, like improving drug solubility and tissue penetration or generating sustained release. Furthermore liposomes have already proven their value for the delivery of PSs in PDT . Hence it is clearly necessary to investigate liposomes as a physiologically inert application form of hypericin.…”

Section: Introductionmentioning

confidence: 99%

Hypericin Loaded Liposomes for Anti‐Microbial Photodynamic Therapy of Gram‐Positive Bacteria

Plenagl¹,

Seitz²,

Pinnapireddy³

et al. 2018

Physica Status Solidi (a)

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Due to its highly lipophilic nature, poor solubility in aqueous media, and poor bioavailability, the naturally occurring photosensitizer hypericin has a limited therapeutic value. Liposomal encapsulation is a promising solution to overcome these limitations. Use of liposomes as delivery vehicles for hypericin in antimicrobial photodynamic therapy (aPDT) is quite new. The aim of this work is therefore, to prepare various hypericin loaded liposomal formulations viz. DOPE/CHEMS/DPPC, DSPC/DPPC/DSPE-PEG, and DPPC/ DOTAP. The formulations are physicochemically characterized and tested for their binding affinity toward bacteria and for their photodynamic efficiency. All formulations achieve a 2.3-2.5 log reduction of Staphylococcus saprophyticus subsp. bovis and facilitate the binding and uptake of the photosensitizer through the bacterial cell wall.

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Photo-responsive tetraether lipids based vesicles for prophyrin mediated vascular targeting and direct phototherapy

Cited by 19 publications

References 36 publications

Stabilized tetraether lipids based particles guided prophyrins photodynamic therapy

Stabilized tetraether lipids based particles guided prophyrins photodynamic therapy

Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes

Hypericin Loaded Liposomes for Anti‐Microbial Photodynamic Therapy of Gram‐Positive Bacteria

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