Photoaction upon adipose tissue cells in vitro

Dubrovskii, V. A.; Dvorkin, B. A.; Yanina, Irina Yu.; Tuchin, Valery V.

doi:10.1134/s1990519x1105004x

Cited by 12 publications

(11 citation statements)

References 23 publications

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“…The result was also the cell lipolysis. The hypothesis of pore formation is in good agreement with the experimental results [37, 206,220,224]. Fig.…”

Section: Photochemical and Photothermal Clearingsupporting

confidence: 81%

“…Reversible membrane injury consists in the creation of new pores or enlargement of the already existing ones, which facilitates efficient exchange between the cell content and the environment. In fat cells the presence of pores increases the lipolysis, as a result of which the intercellular space becomes filled with the cell content and decay products (triglycerides, fatty acids, water and glycerol) [37, [220][221][222][223]. The appearance of such immersion fluid in the intercellular space facilitates the process of optical clearing of the adipose tissue [37].…”

Section: Photochemical and Photothermal Clearingmentioning

confidence: 99%

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Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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Abstract.A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical properties, particularly, the reduction of light scattering in tissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes. © 2015 Samara State Aerospace University (SSAU).Keywords: tissue, optical clearing, optical diagnostics, imaging. 1, 404-413 (1997). 5. C. L. Smithpeter, A. K. Dunn, A. J. Welch, and R. Richards-Kortum, "Penetration depth limits of in vivo confocal reflectance imaging," Appl. Opt. 37, 2749Opt. 37, -2754Opt. 37, (1998. 6. V. V. Tuchin, L. V. Wang, and D. A. Zimnyakov, Optical Polarization in Biomedical Applications, SpringerVerlag, New York, NY, USA (2006). 7. R. Drezek, A. Dunn, and R. Richards-Kortum, "Light scattering from cells: finite-difference time-domain simulations and goniometric measurements," Appl. Opt. 38(16), 3651-3661 (1999). 8. K. Sokolov, R. Drezek, K. Gossagee, and R. Richards-Kortum, "Reflectance spectroscopy with polarized light:is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999). 9. D. W. Leonard, and K. M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," Biophysical J. 72, 1382-1387 (1997). 10. A. G. Borovoi, E. I. Naats, and U. G. Oppel, "Scattering of light by a red blood cell," J. Biomed. Opt. 3, 364-372 (1998). 11. A. N. Yaroslavsky, A. V. Priezzhev, J. Rodriguez, I. V. Yaroslavsky, and H. Battarbee, "Optics of blood,"Chap. 2 in Handbook of Optical Biomedical Diagnostics, V. V. Tuchin, Ed., pp. 169-216, PM107 SPIE Press, Bellingham, WA, USA (2002). 12. G. Mazarevica, T. Freivalds, and A. Jurka, "Properties of erythrocyte light refraction in diabetic patients," J.Biomed. Opt. 7, 244-247 (2002). 13. M. Friebel, and M. Meinke, "Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration," Appl. Opt. 45(12), 2838-2842 (2006). Appl. Opt. 35(19), 3413-3420 (1996). 34. D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, "Imaging dermal blood flow through the intact rat skin with an optical clearing method," J. Biomed. Opt. 15, 026008 (2010 241. K. König, G. Flemming, and R. Hibst, "Laser-induced autofluorescence spectroscopy of dental caries lesion," Cell. Mol. Biol. 44, 1293-1300. 242. E. G. Borisova, T. T. Uzunov, and L. A. Avramov, "Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy," Lasers Surg. Med. 34, 249-253 (2004 -20(12), 1512-1516 (1984). 245. K. Konig, H. Schneckenburger, and R. Hibst, "Time-gated in vivo autofluorescence imaging of dental caries,"Cell. Mol. Biol. 45, 233-239 (1999). 246. E. Borisova, P. Tr...

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“…The result was also the cell lipolysis. The hypothesis of pore formation is in good agreement with the experimental results [37, 206,220,224]. Fig.…”

Section: Photochemical and Photothermal Clearingsupporting

confidence: 81%

Section: Photochemical and Photothermal Clearingmentioning

confidence: 99%

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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“…When cooling the samples with the temperature T L 36 °C (open symbols), the slope of the dependences becomes smoother than that during heating. This may indicate that a partial degradation of the fatty tissue, for example, due to its lipolysis [39,40], apparently occurs at any temperature exceeding the temperature of a living organism.…”

Section: Porcine Fatty Tissuementioning

confidence: 99%

In-vitro study of the temperature dependence of the optoacoustic conversion efficiency in biological tissues

Nikitin¹,

Khokhlova²,

Pelivanov³

2012

Quantum Electron.

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The applicability of the optoacoustic (OA) method for monitoring temperature during thermal impact on biological tissues is studied experimentally. Tissues under study were chicken breast (as a model of muscle), porcine lard (as a model of fatty tissue) and porcine liver (as a model of richly perfused tissue). The temperature dependences of the amplitude of the OA signals excited in biologi cal tissues were measured in-vitro in the temperature range of 20 -80 °C under the narrow laser beam conditions. Measurements were performed in two regimes: during heating and cooling. Simi larities and differences in the behaviour of the dependences in dif ferent temperature ranges associated with different structural com position of the samples were obtained. The accuracy of temperature reconstruction from experimental data for the investigated tissue types was evaluated. It is shown that during tissue coagulation its temperature can be determined with an accuracy of about 1 °C.

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“…The latter can act as gateways for free fat acids (FFAs) leakage outside the cell, because the molecular size of FFAs does not exceed 1À2 nm in diameter. 20 Cell lipolysis can be observed as optical clearing of photodynamically/photothermally modi¯ed fat tissue samples. 20 Due to light-induced cell membrane porosity, the intracellular content of the cell (FFAs) percolates through the arising temporal pores into the interstitial space.…”

Section: à18mentioning

confidence: 99%

“…20 Cell lipolysis can be observed as optical clearing of photodynamically/photothermally modi¯ed fat tissue samples. 20 Due to light-induced cell membrane porosity, the intracellular content of the cell (FFAs) percolates through the arising temporal pores into the interstitial space. As a consequence, the refractive index of the interstitial°uid (initially equal to n i ¼ 1:36) 21 becomes closer to the refractive index inside the adipocytes (fat refractive index, n a ¼ 1:44) 22 and due to the refractive index matching e®ect the optical medium becomes optically more homogeneous and more transparent to light.…”

Section: à18mentioning

confidence: 99%

OPTICAL COHERENCE TOMOGRAPHY OF ADIPOSE TISSUE AT PHOTODYNAMIC/PHOTOTHERMAL TREATMENT IN VITRO

Yanina

Trunina

Tuchin

2013

J. Innov. Opt. Health Sci.

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Temporal changes in structure and refractive-index distribution of adipose tissue at photodynamic/photothermal treatment were studied with OCT in vitro. EthanolÀwater solutions of indocyanine green (ICG) and brilliant green (BG) were used for fat tissue staining. CW laser diode (808 nm) and LED light source (442 and 597 nm) were used for irradiation of stained tissue slices. The data received supporting the hypothesis that photodynamic/photothermal treatment, induces fat cell lipolysis during a certain period of time after light exposure.

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Photoaction upon adipose tissue cells in vitro

Cited by 12 publications

References 23 publications

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

In-vitro study of the temperature dependence of the optoacoustic conversion efficiency in biological tissues

OPTICAL COHERENCE TOMOGRAPHY OF ADIPOSE TISSUE AT PHOTODYNAMIC/PHOTOTHERMAL TREATMENT IN VITRO

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