Photobiomodulation preserves behaviour and midbrain dopaminergic cells from MPTP toxicity: evidence from two mouse strains

Moro, Cécile; Torres, Napoléon; Massri, Nabil El; Ragazzoni, David; Johnstone, Daniel M.; Stone, Jonathan; Mitrofanis, John; Benabid, Alim‐Louis

doi:10.1186/1471-2202-14-40

Cited by 60 publications

(99 citation statements)

References 31 publications

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“…Our experimental paradigm of the simultaneous administration of parkinsonian insult and treatment was similar to that in previous animal models of Parkinson disease. 23,25,33,37,38,43 This paradigm is unlike the clinical reality in which there is cell loss prior to therapeutic intervention; however, in our experimental study we hoped to determine the maximum effect of any NIr neuroprotection provided via the LED-optical fiber implant.…”

Section: Neuroprotection Of the Snc By Nir After Mptp Treatmentmentioning

confidence: 99%

“…This ensured that each device emitted a similar output from the optical fiber, which was 0.16 mW (at 20 mA, maximum electronic power of the battery for this device). We preferred to use an LED instead of a laser device in these and most of the other experiments for several reasons: 1) it was more easily attached to the optical fiber, 2) the external WARP device used in previous studies 25,32,38 was an LED (670 nm) and thus allowed us to make a direct comparison, and 3) we found that the LED emitted no heat across the implant and cranium and was satisfactory for use over longer periods (6 days).…”

Section: Feasibility Of the Device: Delivering Nir Into The Mouse Brainmentioning

confidence: 99%

“…23,25,37,38 At 15 minutes and 6 hours after each injection, the LED was turned on in the mice in some of the experimental groups. In the Saline-pNIr and MPTP-pNIr groups, the LED was turned on for a 90-second pulse (same duration as in previous studies using the external WARP-LED apparatus), 25,32,37,38 whereas in the Saline-cNIr and MPTP-cNIr groups, the LED was turned on and left on until the end of the experimental period (6 days). Following the experimental period, the mice were anesthetized, and their brains (midbrain sections including the SNc) were processed for TH immunocytochemistry as described above.…”

Section: Neuroprotection Of the Snc By Nir After Mptp Treatmentmentioning

confidence: 99%

“…37 Further, NIr treatment recovers various parameters of locomotive behavior, for example, mobility and velocity, in MPTP-treated mice. 25,44 Moreover, in transgenic mice expressing the A53T human a-synuclein mutation, NIr treatment delays disease progression and reduces the severity of the disease phenotype. 34 In addition to these in vivo studies are many in vitro studies that have shown that NIr protects rat striatal and cortical cells, 22,46 as well as human neuroblastoma cells engineered to overexpress a-synuclein, 34 against the toxic effects of MPTP.…”

mentioning

confidence: 99%

“…To this end, we here describe the use of a novel LED-optical fiber device made to deliver NIr intracranially, within the mouse brain. Specifically, we wanted to determine several issues related to this device: 1) feasibility, that is, whether the device was viable in the living brain ; 2) NIr signal attenuation within the living brain, that is, whether light levels changed at various distances from the source; 3) toxicity, that is, whether tissue necrosis occurred at the implant site; and 4) neuroprotection, that is, whether intracranially applied NIr, as with extracranially applied NIr, 25,32,38 was neuroprotective to SNc dopaminergic cells after MPTP treatment.…”

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confidence: 99%

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Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice

Moro

Massri²,

Torres

et al. 2014

JNS

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Object Previous experimental studies have documented the neuroprotection of damaged or diseased cells after applying, from outside the brain, near-infrared light (NIr) to the brain by using external light-emitting diodes (LEDs) or laser devices. In the present study, the authors describe an effective and reliable surgical method of applying to the brain, from inside the brain, NIr to the brain. They developed a novel internal surgical device that delivers the NIr to brain regions very close to target damaged or diseased cells. They suggest that this device will be useful in applying NIr within the large human brain, particularly if the target cells have a very deep location. Methods An optical fiber linked to an LED or laser device was surgically implanted into the lateral ventricle of BALB/c mice or Sprague-Dawley rats. The authors explored the feasibility of the internal device, measured the NIr signal through living tissue, looked for evidence of toxicity at doses higher than those required for neuroprotection, and confirmed the neuroprotective effect of NIr on dopaminergic cells in the substantia nigra pars compacta (SNc) in an acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of Parkinson disease in mice. Results The device was stable in freely moving animals, and the NIr filled the cranial cavity. Measurements showed that the NIr intensity declined as distance from the source increased across the brain (65% per mm) but was detectable up to 10 mm away. At neuroprotective (0.16 mW) and much higher (67 mW) intensities, the NIr caused no observable behavioral deficits, nor was there evidence of tissue necrosis at the fiber tip, where radiation was most intense. Finally, the intracranially delivered NIr protected SNc cells against MPTP insult; there were consistently more dopaminergic cells in MPTP-treated mice irradiated with NIr than in those that were not irradiated. Conclusions In summary, the authors showed that NIr can be applied intracranially, does not have toxic side effects, and is neuroprotective.

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Section: Neuroprotection Of the Snc By Nir After Mptp Treatmentmentioning

confidence: 99%

Section: Feasibility Of the Device: Delivering Nir Into The Mouse Brainmentioning

confidence: 99%

Section: Neuroprotection Of the Snc By Nir After Mptp Treatmentmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice

Moro

Massri²,

Torres

et al. 2014

JNS

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Near‐infrared light is neuroprotective in a monkey model of Parkinson disease

Darlot

Moro

Massri

et al. 2015

Annals of Neurology

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Objective: To examine whether near-infrared light (NIr) treatment reduces clinical signs and/or offers neuroprotection in a subacute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) monkey model of Parkinson disease. Methods: We implanted an optical fiber device that delivered NIr (670nm) to the midbrain of macaque monkeys, close to the substantia nigra of both sides. MPTP injections (1.5-2.1mg/kg) were made over a 5-to 7-day period, during which time the NIr device was turned on. This was then followed by a 3-week survival period. Monkeys were evaluated clinically (eg, posture, bradykinesia) and behaviorally (open field test), and their brains were processed for immunohistochemistry and stereology. Results: All monkeys in the MPTP group developed severe clinical and behavioral impairment (mean clinical scores 5 21-34; n 5 11). By contrast, the MPTP-NIr group developed much less clinical and behavioral impairment (n 5 9); some monkeys developed moderate clinical signs (mean scores 5 11-15; n 5 3), whereas the majority-quite remarkably-developed few clinical signs (mean scores 5 1-6; n 5 6). The monkeys that developed moderate clinical signs had hematic fluid in their optical fibers at postmortem, presumably limiting NIr exposure and overall clinical improvement. NIr was not toxic to brain tissue and offered neuroprotection to dopaminergic cells and their terminations against MPTP insult, particularly in animals that developed few clinical signs. Interpretation: Our findings indicate NIr to be an effective therapeutic agent in a primate model of the disease and create the template for translation into clinical trials.

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Recent advances in photobiomodulation therapy for brain diseases

Dong,

Ren,

Zhang

et al. 2024

Interdisciplinary Medicine

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Light therapy techniques, such as photobiomodulation therapy (PBMT), photodynamic therapy (PDT), and laser photoablation, have gained widespread attention and become indispensable physiotherapy methods in clinical practice. PBMT involves the application of low‐level laser/LED to modulate the function of nerve cells, relieve neuroinflammation, promote neurogenesis and vascular growth. Recent studies have shown that PBMT holds promise as a complementary or alternative treatment of Alzheimer's disease (AD), traumatic brain injury (TBI), major depressive disorder (MDD), etc. However, the therapeutic effect of PBMT is influenced by various factors, such as the patients' condition, brain structure and function, illumination parameters, etc. Therefore, the optimized parameters, personalized therapeutic schedules, and precise evaluation of the therapeutic effect are crucial to the treatment success. In this review, we identified the recent experimental and clinical successes, existing obstacles, and future opportunities for PBMT in the treatment of the brain diseases. As a non‐invasive, side‐effect‐free, and highly accessible technique, PBMT brings a glimmer of light for the treatment of neuropsychiatric disorders and the neuro‐rejuvenation of human brains.

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Photobiomodulation preserves behaviour and midbrain dopaminergic cells from MPTP toxicity: evidence from two mouse strains

Cited by 60 publications

References 31 publications

Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice

Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice

Near‐infrared light is neuroprotective in a monkey model of Parkinson disease

Recent advances in photobiomodulation therapy for brain diseases

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