Photochemical internalization provides time- and space-controlled endolysosomal escape of therapeutic molecules

Selbo, Pål Kristian; Weyergang, Anette; Høgset, Anders; Norum, Ole Jacob; Berstad, Maria Brandal; Vikdal, Marie; Berg, Kristian

doi:10.1016/j.jconrel.2010.06.008

Cited by 252 publications

(235 citation statements)

References 98 publications

Supporting

Mentioning

223

Contrasting

Unclassified

Order By: Relevance

“…MHT alone most probably facilitates enhanced drug efficacy by increasing cellular uptake via endocytosis. Both PCI and MHT have been shown to promote endosomal escape and the release of drug into the cytosol [16,19]. In addition, both drugs used in this study are incorporated into DNA causing damage which often results in cell death unless the damage is repaired.…”

Section: Combined Pci Mediated Chemotherapy and External Induced Mhtmentioning

confidence: 99%

“…PCI, based on the principles of PDT, is a technique that improves the utilization of macromolecules in cancer therapy in a site-specific manner [15][16][17][18]. The concept is based on the use of specially designed photosensitizers, with two sulfonate groups on adjacent phenyl rings such as TPPS2a AlPcS2a and TPCS2a, that localize preferentially in the membranes of endocytic vesicles.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synergistic chemotherapy by combined moderate hyperthermia and photochemical internalization

Christie

Molina

Gonzales

et al. 2016

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Combination therapies of photochemical internalization (PCI) and moderate hyperthermia (MHT) were investigated in an in vitro system consisting of human and rat glioma spheroids. PCI using the amphiphilic photosensitizer, AlPcS2a and two anti cancer agents BLM or 5-FU were used. Spheroids were irradiated with λ = 670 nm laser light in an incubator at temperatures ranging from 37 to 44°C. For each temperature investigated, spheroids were divided into 4 groups: control, drug-only, photodynamic therapy (PDT), and PCI. PDT and PCI spheroids were exposed to radiant exposures ranging from 0.3 to 2.5 J cm −2 using an irradiance of 5 mW cm −2 . Toxicity was evaluated from spheroid growth kinetics. The combination of PCI and MHT resulted in significant increases in BLM efficacy at 44°C for both cell line derived spheroids compared to controls at 37°C over the range of radiant exposures examined. 5-FU PCI was ineffective for the human cell line at both 37 and 44°C. L. West, "Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance," Proc. Natl. Acad. Sci. U.S.A. 100(23), 13549-13554 (2003). 31. X. Huang, P. K. Jain, I. H. El-Sayed, and M. A. El-Sayed, "Determination of the minimum temperature required for selective photothermal destruction of cancer cells with the use of immunotargeted gold nanoparticles," Photochem. Photobiol. 82(2), 412-417 (2006). 32.

show abstract

Section: Combined Pci Mediated Chemotherapy and External Induced Mhtmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synergistic chemotherapy by combined moderate hyperthermia and photochemical internalization

Christie

Molina

Gonzales

et al. 2016

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…Photosensitizers that are employed in the PCI technology are usually amphiphilic compounds which can bind to and localize in the plasma membrane. After being taken up by the cells through endocytosis, the photosensitizers are confined to the endosomal membranes and remain inactive until triggered by light with specific wavelengths matching their absorption spectra (Selbo, Weyergang et al 2010). Once activated, they induce the formation of highly reactive oxygen species, mainly singlet oxygen, leading to the rupture of endosomes and lysosomes membrane.…”

Section: Photochemical Internalization (Pci)mentioning

confidence: 99%

“…PCI principally targets cellular endocytosis that may affect the distribution of molecules that are taken up by the cells via endosomal pathway. It was later employed as a tool to improve the cellular delivery of a large variety of bioactive macromolecules and nucleic acids including pDNA, siRNA and oligonucleotides (Selbo, Weyergang et al 2010). Examples of photosensitizers that are used in non-viral nucleic acid delivery including disulfonated meso-tetraphenylporphine (TPPS2a) (Prasmickaite, Høgset et al 2000;Kloeckner, Prasmickaite et al 2004;Maurice-Duelli, Ndoye et al 2004;Ndoye, Merlin et al 2004;Ndoye, Dolivet et al 2006;Oliveira, Fretz et al 2007;Boe, Longva et al 2008;Raemdonck, Naeye et al 2009;Bøe, Saebøe-Larssen et al 2010), disulfonated aluminium phthalocyanine (AlPcS2a) (Berg, Prasmickaite et al 2003;Hellum, Høgset et al 2003;Ndoye, Dolivet et al 2006;Yip, Weyergang et al 2007), Zincphthalocyanine (Zn-Pc) dendrimer (Nishiyama, Iriyama et al 2005;Arnida, Nishiyama et al 2006) and 5,10,15-tri(4-acetamidophenyl)-20-mono(4-carboxyl-phenyl)porphyrin (TAMCPP) conjugated to G4 PAMAM dendrimer (Shieh, Peng et al 2008).…”

Section: Photochemical Internalization (Pci)mentioning

confidence: 99%

Endosomal Escape Pathways for Non-Viral Nucleic Acid Delivery Systems

Liang¹,

Lam²

2012

Molecular Regulation of Endocytosis

122

View full text Add to dashboard Cite

“…Berg et al developed photochemical internalization (PCI), a novel method to release drugs in endocytic vesicles to enhance the therapeutic effects ( Figure 6) [34]. PCI is now a well-established method and has been applied to enhance the delivery various therapeutic molecules [35][36][37]. While PCIuses random oxidation of endo/lysosomal membranes to release the contents from the vesicles, chemically controlled approachescan also release drugs using photodynamic release.…”

Section: Photodynamic Releasementioning

confidence: 99%

Emerging Strategies for Controlling Drug Release by Using Visible/Near IR Light

Bio¹,

You²

2013

Med Chem

View full text Add to dashboard Cite

Effective drug delivery systems require controlled drug release at the target cancer cell. While strategies for targeting tumors have been extensively studied, a better understanding of the necessary technology for controlling the spatiotemporal release of a drug is still needed. It has been established that the use of light can be a unique tool for controlling drug release. While UV light can be used for the release of biologically active, caged (deactivated) compounds, clinical application is restricted because of its limited ability to penetrate tissues as well as its cytotoxicity. Recently, the use of both tissue-penetrable visible and near IR have shown promise to overcome these limitations. In this short review, we introduce new smart strategies to convert such low energy light to a tissue-penetrable stimulus for both actively and remotely controlling drug release.

show abstract

Photochemical internalization provides time- and space-controlled endolysosomal escape of therapeutic molecules

Cited by 252 publications

References 98 publications

Synergistic chemotherapy by combined moderate hyperthermia and photochemical internalization

Synergistic chemotherapy by combined moderate hyperthermia and photochemical internalization

Endosomal Escape Pathways for Non-Viral Nucleic Acid Delivery Systems

Emerging Strategies for Controlling Drug Release by Using Visible/Near IR Light

Contact Info

Product

Resources

About