2002
DOI: 10.1039/b205471j
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Photodynamic parameters in the chick chorioallantoic membrane (CAM) bioassay for photosensitizers administered intraperitoneally (IP) into the chick embryo

Abstract: The chick chorioallantoic membrane (CAM) assay was used to determine the photodynamic response (PDR) of blood vessels to Photofrin, 5-aminolevulinic acid (ALA), benzoporphyrin derivative monoacid ring A (BPD-MA) and lutetium texaphyrin (Lutex). The photosensitizers were administered systemically via intraperitoneal injection into the chick embryo. Forward stepwise regression analysis of the PDR results enabled the individual contributions of seven experimental variables to be ranked: drug dose, light dose, flu… Show more

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Cited by 25 publications
(26 citation statements)
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“…Taking these issues into account and in an effort to maximize the benefits of both PDL and PDT approaches, we have designed a protocol for treatment of cutaneous vascular lesions [18] whereby we initiate therapy with PDT exposure, using yellow light (l ¼ 576 nm) absorbed by the photosensitizer, benzoporphyrin derivative monoacid ring A (BPD), causing initial vascular damage and presumably leaving PWS blood vessels more vulnerable to subsequent photothermal damage [19]. PDL irradiation is then used to heat selectively the pre-treated vessels compromised by PDT.…”
Section: Introductionmentioning
confidence: 99%
“…Taking these issues into account and in an effort to maximize the benefits of both PDL and PDT approaches, we have designed a protocol for treatment of cutaneous vascular lesions [18] whereby we initiate therapy with PDT exposure, using yellow light (l ¼ 576 nm) absorbed by the photosensitizer, benzoporphyrin derivative monoacid ring A (BPD), causing initial vascular damage and presumably leaving PWS blood vessels more vulnerable to subsequent photothermal damage [19]. PDL irradiation is then used to heat selectively the pre-treated vessels compromised by PDT.…”
Section: Introductionmentioning
confidence: 99%
“…22) Hemorrhage is included as a parameter for phototoxic damage, and designated as VRPϭϪ100 %. This type of damage was previously weighted as the highest score 12,22) as well. HY-PDT induced hemorrhagic damage was only observed in HD group.…”
Section: Fig 7 Vrp Represented By Box-plots Of Hc Hd and Ld Groupsmentioning
confidence: 99%
“…When a living model in anti-angiogenesis study was used, most publications did not mention the change of vessels prior to and after intervention such as the application of various bioactives. 12,21,22,26) In addition to the above mentioned study design, this present study took two major steps to minimize variations. Firstly, standardization of the image capturing procedure and its subsequent image analysis was implemented to reduce the variations in image capturing.…”
Section: Fig 7 Vrp Represented By Box-plots Of Hc Hd and Ld Groupsmentioning
confidence: 99%
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“…Ideal characteristics for the photosensitizer are low dark toxicity, high efficiency for singlet oxygen generation, high penetration by cell membranes and fast post-treatment clearance [23][24][25][26][27][28][29]. Other relevant characteristics are needed to a molecule become a clinical photosensitizer, such as a long life-time of the excited triplet state and high molar absorbance at the electromagnetic ''therapeutic window'' between 600 nm and 1000 nm, where the light show a measurable penetration into the biological tissues [30][31][32][33].…”
Section: Introductionmentioning
confidence: 99%