Photodynamic therapy of early squamous cell carcinoma with tetra(m-hydroxyphenyl)chlorin: optimal drug-light interval

Andrejevic‐Blant, Snezana; Hadjur, Christophe; Ballini, Jean‐Pierre; Wagnières, Georges; Fontolliet, C; Bergh, Hubert van den; Monnier, Ph.

doi:10.1038/bjc.1997.502

Cited by 46 publications

(33 citation statements)

References 26 publications

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“…At 24 h, strong fluorescence was seen in the cytoplasm of tumour cells, but not in the surrounding muscle cells. Results from studies using a Syrian hamster cheek pouch tumour model (Andrejevic-Blant et al, 1997) demonstrated high fluorescence levels in endothelial cells at 8 h after Foscan, with accumulation in squamous epithelial tissues from 2 to 4 days. Clinical studies in a group of patients treated with Foscan PDT for gynaecological cancers (Wierrani et al, 1997) or SCC of the upper airways (Andrejevic Blant et al, 2001) also demonstrated high fluorescence levels in endothelial and inflammatory cells during the first 20 h.…”

Section: Discussionmentioning

confidence: 99%

Foscan® uptake and tissue distribution in relation to photodynamic efficacy

et al. 2003

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Clinical photodynamic therapy (PDT) schedules are based on the assumption that optimum drug -light intervals are times at which there is a maximum differential between photosensitiser retention in the tumour and surrounding normal tissue. However, vascularmediated effects contribute to tumour destruction by PDT; therefore, plasma sensitiser levels and endothelial cell drug exposure could also be important determinants of PDT response. The purpose of this study was to investigate the influence of tumour, tissue and plasma concentrations of the photosensitiser Foscan s (meta-tetrahydroxyphenylchlorin, mTHPC) on PDT response. Groups of BalbC nude mice, bearing human mesothelioma xenografts (H-MESO1) were injected (i.v.) with a single dose of 14 C-labelled mTHPC, or with two doses, separated by 72 h. Drug levels in plasma, tumour and normal tissues were measured at 5 min to 120 h after drug administration. The PDT tumour and skin responses were evaluated by illuminating separate groups mice at intervals of 5 min to 120 h after injection of Foscan (nonlabelled). Drug levels in both tumour and skin increased during the first 24 h after a single injection, and remained almost constant for at least 120 h. The second injection produced a further, rapid increase in mTHPC levels in tumours and skin, with steady state being maintained from 20 min to 120 h. By contrast, PDT response of both tumours and skin were maximal for illumination at 1 -3 h after drug, with very little response when illumination was given 48 -120 h after drug. There was no significant correlation between tumour or skin drug level and PDT response. There was, however, a significant correlation between plasma drug levels and tumour or skin response, excluding an initial distribution time of 20 min. These studies demonstrate a pronounced disassociation between tumour drug levels and optimum drug -light intervals for PDT response with Foscan. We suggest that the PDT effect, in both tumours and normal tissues, is largely mediated via vascular damage and that the selectivity of PDT is not based on differential tumour drug uptake.

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Section: Discussionmentioning

confidence: 99%

Foscan® uptake and tissue distribution in relation to photodynamic efficacy

et al. 2003

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“…This result is in contrast to other PSs such as mTHPC, which provide a contrast in favour of the epithelial tissue at long DLI (450 h). It should be noted that this 50-h DLI is much longer than those considered in the present study (Andrejevic-Blant et al, 1997a.…”

Section: Discussionmentioning

confidence: 50%

“…In order to evaluate the depth and extent of the PDT treatment, we used a tissue damage scale graded from 0 to 4. The scaling is based on the lateral extension of the macroscopic necrosis, the formation of fibrin and the in-depth extension of the necrosis to the different mucosal layers after histological examination of the tissues sections, in a similar way to the previous studies with different PSs (Andrejevic-Blant et al, 1997a, Rosenbach-Belkin et al, 1998Zellweger et al, 2000). Histologically, the tissue damage scale is related with the number of layers of tissue that are necrosed as well as the lateral extension of the necrosis.…”

Section: Evaluation Of the Photodynamic Effectmentioning

confidence: 92%

“…The treatment of tumours requires a knowledge of the levels of response of the healthy tissue after the PDT in order to choose the conditions that are not highly necrotic for healthy tissue. Similar tissue damage scales were used in several studies of the PDT effects of other PSs such as Foscan s (Andrejevic-Blant et al, 1997a.…”

Section: Macroscopic and Histological Aspects Of The Tissue Reactionmentioning

confidence: 99%

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Selectivity of the photosensitiser Tookad® for photodynamic therapy evaluated in the Syrian golden hamster cheek pouch tumour model

et al. 2003

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The response to photodynamic therapy (PDT) with the photosensitiser (PS) Tookad s was measured in the Syrian hamster cheek pouch model on normal mucosae and chemically induced squamous cell carcinoma. This PS is a palladium-bacteriopheophorbide presenting absorption peaks at 538 and 762 nm. The light dose, drug dose and drug injection-light irradiation times (DLI), ranging between 100 and 300 J cm À2 , 1 -5 mg kg À1 and 10 -240 min respectively, were varied and the response to PDT was analysed by staging the macroscopic response and by the histological examination of the sections of the irradiated cheek pouch. A fast time decay of the tissular response with drug dose of 1 -5 mg kg À1 was observed for DLI ranging from 10 to 240 min and for light doses of 100 -300 J cm À2 delivered at a light dose rate of 150 mW cm À2 . A significantly higher level of tissular response was observed for squamous cell carcinoma compared to normal tissue. Nevertheless, the threshold level of the drug -light dose for a detectable response was not significantly different in the tumoral vs normal tissue. The highest response at the shortest DLIs and the absence of measurable response at DLI larger than 240 min at light dose of 300 J cm À2 and drug dose of 5 mg kg À1 reveals the predominantly vascular effect of Tookad s . This observation suggests that Tookad s could be effective in PDT of vascularised lesions.

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“…We consider that rapid and cancer-selective PS delivery by HVJ-E allowed selective accumulation of the PS even at a short incubation period [24] [25]. Based on this, we performed PDT using PE with high con- In this study, we also evaluated the selectivity of cell death induced by PDT using PE with the 10 min drug-light interval.…”

Section: Discussionmentioning

confidence: 99%

Highly Selective Photodynamic Therapy with a Short Drug-Light Interval Using a Cytotoxic Photosensitizer Porphyrus Envelope for Drug-Resistant Prostate Cancer Cells

Hong¹,

Inai²,

Honda³

et al. 2018

IJCM

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Background: Photodynamic therapy (PDT) is a less invasive cancer treatment using photochemical reactions induced by light irradiation to a photosensitizer (PS). Highly selective PDT with fast accumulation of the PS in target site might be a promising treatment option for drug-resistant prostate cancer facing high incidence rate of elderly men who have no effective treatment options and require a minimally invasive treatment. Hemagglutinating virus of Japan envelope (HVJ-E) allows selective and fast drug delivery to the drugresistant prostate cancer cells via rapid cell membrane fusion. PS named porphyrus envelope (PE) has been developed by insertion of lipidated protoporphyrin IX (PpIX lipid) into HVJ-E. In this study, we investigated the optimal conditions for PE preparation and laser irradiation for highly selective PDT using PE with a short drug-light interval. Materials and Methods: Human hormon refractory prostate cancer cell line PC-3 and human normal prostate epithelial cell line PNT2 were cultured. PpIX lipid uptake and cytotoxicity of PDT in the cells incubated with PE for 10 min were evaluated by measuring fluorescence intensity and by using a cell counting reagent 24 h after PDT, respectively. Results: PpIX lipid uptake and cytotoxicity of PDT were increased with PpIX lipid concentration. Cytotoxicity of PDT using PE was more than 9 times as strong as that with PpIX lipid and PpIX induced by 5-aminolevulinic acid. Much stronger cytotoxicity was induced in PC-3 cells than PNT2 cells with the ratio of cell death rate for cancer to normal cells up to 4.64 ± 0.09. Conclusions: Fast PS delivery with HVJ-E allows highly selective PDT with a short drug-light interval. Therefore, PDT using PE has a potential to shorten treatment period and reduce side effects of PDT.

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Photodynamic therapy of early squamous cell carcinoma with tetra(m-hydroxyphenyl)chlorin: optimal drug-light interval

Cited by 46 publications

References 26 publications

Foscan® uptake and tissue distribution in relation to photodynamic efficacy

Foscan® uptake and tissue distribution in relation to photodynamic efficacy

Selectivity of the photosensitiser Tookad® for photodynamic therapy evaluated in the Syrian golden hamster cheek pouch tumour model

Highly Selective Photodynamic Therapy with a Short Drug-Light Interval Using a Cytotoxic Photosensitizer Porphyrus Envelope for Drug-Resistant Prostate Cancer Cells

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