Photoelectrochemical aptasensor for the sensitive and selective detection of kanamycin based on Au nanoparticle functionalized self-doped TiO₂nanotube arrays

Abstract

In this communication, a new photoelectrochemical aptasensor with Au nanoparticle functionalized self-doped TiO2 nanotube arrays (Au/SD-TiO2 NTs) as the core sensing unit and aptamers as the recognition unit was set up to accomplish the sensitive and selective detection of kanamycin with the lowest detection limit of 0.1 nM.

Photoelectrochemical Immunosensor for Detection of Carcinoembryonic Antigen Based on 2D TiO2 Nanosheets and Carboxylated Graphitic Carbon Nitride

“…Therefore, photoactive materials are crucial for the performance of the PEC sensors. Many photoactive materials are metal-contained semiconductors, such as TiO 2 , CdSe, CdTe, ZnO, ZnS, etc123456789. Among them, titanium dioxide is one of the most commonly employed materials because of its nontoxicity, low-cost and brilliant photochemical and chemical stability.…”

Photoelectrochemical aptasensing

“…Using similar mechanism, they further reported the PEC aptasensing of tetracycline and chloramphenicol, respectively [65,72]. In another work, kanamycin molecules were confined by the aptamers onto the Au NPs-TiO 2 NTs electrode for PEC oxidation [73]. In similar configurations, nevertheless, some other works have observed different phenomena and given different explanations.…”

“…For example, thiol-functionalized aptamer subunits were used for the aptamer-modified gold electrodes in the work reported by Willner's group [49]. In a recent work, mercapto linked probe (HS-aptamer) was used for its immobilization on an Au NPs/TiO 2 NTs electrode [73]. The interaction between Au and NH 2 -aptamer has also been reported [61].…”

“…It is worth noting that some interesting hybrid materials, e.g. Au NPs/selfdoped TiO 2 NTs [73] and N-doped carbon QDs/TiO 2 nanorods [81], have also been exploited for advanced PEC aptasensing.…”

Nanosheet‐Stacked Chiral Silica Transcribed from Metal Ion‐ and pH‐Tuned Supramolecular Crystalline Complexes of Polyamine‐D‐Glucarate

“…As it is well known, aptamers refer to single‐stranded DNA or RNA oligonucleotides that can selectively bind to a broad range of targets, including metal ions, small molecules, peptides, proteins, and even complex targets such as whole cells and materials surfaces 22, 23. Aptamers are selected using systematic evolution of ligands by exponential enrichment (SELEX) 24, 25. Owing to numerous advantages such as high stability (especially DNA aptamers), small size, ease of modification, high binding affinity, high specificity and wide target range, aptamers are suitable candidates as recognition elements for the construction of biosensors 22, 26, 23–25.…”