Photoinactivation of Influenza Virus Fusion and Infectivity by Rose Bengal

Lenard, John; Vanderoef, Roger

doi:10.1111/j.1751-1097.1993.tb04926.x

Cited by 42 publications

(25 citation statements)

References 20 publications

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“…Na,K-ATPase was inactivated by light in the presence of RB. 23,24) Acetylcholineesterase and some microorganims, such as Escherichia coli, Staphylococcus aureus, and influenza virus, are inactivated, 17,18,22,25) therefore, it is possible that xanthene dyes inactivate drug-metabolizing enzymes. 26) In the previous study, we clarified that RB inhibited the activity of UGT1A6 and CYP2A6 by mixed-type inhibitory mechanism.…”

Section: Introductionmentioning

confidence: 99%

Inhibition Mechanism of UDP-Glucuronosyltransferase 1A6 by Xanthene Food Dyes

Uesugi

Furumiya

Mizutani

2006

JOURNAL OF HEALTH SCIENCE

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We have reported that erythrosine (ET), a xanthene dye, inhibited uridine 5-diphosphate (UDP)-glucuronosyltransferase 1A6 (UGT1A6). In order to clarify the structure-inhibition relationships of these xanthene dyes, the inhibitory effect of xanthene dye on human UGT1A6 activity was investigated, such as acid red (AR), ET, phloxine (PL), and rose bengal (RB). ET, PL, and RB strongly inhibited human UGT1A6 activity, with IC 50 values = 0.05, 0.04, and 0.015 mM, respectively. Meanwhile, AR had almost no effect (IC 50 value = 1.7 mM). ET, PL and RB have four halogen atoms on their xanthene backbone, unlike AR. Meanwhile, some contrast media with high halogens on those aromatic compounds, such as ioxaglic acid, iodixanol, meglumine iotalamate, and diatriazole sodium, did not inhibit human UGT1A6 activity. These results suggest that halogens enhance the inhibitory effect of xanthene dyes. In this study, it was proved that xanthene dyes had an inhibitory effect on human UGT1A6 activity by the combination of xanthene structure and halogens on its. Part of this inhibition by xanthene dyes depends the reaction of 1 O 2 originated on xanthene dyes by light irradiation, because the inhibition was prevented by 1 O 2 quenchers, such as NaN 3 and histidine, and in the dark.

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Section: Introductionmentioning

confidence: 99%

Inhibition Mechanism of UDP-Glucuronosyltransferase 1A6 by Xanthene Food Dyes

Uesugi

Furumiya

Mizutani

2006

JOURNAL OF HEALTH SCIENCE

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“…In our study, the rose bengal was immobilized by covalent binding to the fibers of the wipes, thus not allowing for enrichment of the photosensitizer in the viral envelope. The prompt inactivation of the influenza viruses on IPS wipes suggests the relevance of singlet oxygen in the pathogen inactivation, as was shown before for other enveloped viruses (58,59) and for bacteria such as Escherichia coli (60). Yet, we could not show that the efficient inactivation of influenza virus was due to released singlet oxygen or to other mechanisms (e.g., antiviral effect of the coating), as complete viral decay on IPS wipes was immediate (t ϭ 0) and therefore also no infectious influenza viruses could be detected on wipes stored in the dark.…”

Section: Discussionmentioning

confidence: 75%

“…Unlike noroviruses and adenoviruses, influenza viruses have a lipoprotein envelope, which makes them less tolerant to disinfectants than nonenveloped viruses, as has been shown for several disinfectants (5,30,57). In a study on the virucidal activity of rose bengal on influenza viruses, it was proposed that singlet oxygen had a direct effect on the fusion protein of the virus after enrichment of the photosensitizer rose bengal in the viral envelope (58). In our study, the rose bengal was immobilized by covalent binding to the fibers of the wipes, thus not allowing for enrichment of the photosensitizer in the viral envelope.…”

Section: Discussionmentioning

confidence: 99%

Wipes Coated with a Singlet-Oxygen-Producing Photosensitizer Are Effective against Human Influenza Virus but Not against Norovirus

Verhaelen

Bouwknegt

Rutjes

et al. 2014

Appl Environ Microbiol

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bTransmission of enteric and respiratory viruses, including human norovirus (hNoV) and human influenza virus, may involve surfaces. In food preparation and health care settings, surfaces are cleaned with wipes; however, wiping may not efficiently reduce contamination or may even spread viruses, increasing a potential public health risk. The virucidal properties of wipes with a singlet-oxygen-generating immobilized photosensitizer (IPS) coating were compared to those of similar but uncoated wipes (non-IPS) and of commonly used viscose wipes. Wipes were spiked with hNoV GI.4 and GII.4, murine norovirus 1 (MNV-1), human adenovirus type 5 (hAdV-5), and influenza virus H1N1 to study viral persistence. We also determined residual and transferred virus proportions on steel carriers after successively wiping a contaminated and an uncontaminated steel carrier. On IPS wipes only, influenza viruses were promptly inactivated with a 5-log 10 reduction. D values of infectious MNV-1 and hAdV-5 were 8.7 and 7.0 h on IPS wipes, 11.6 and 9.3 h on non-IPS wipes, and 10.2 and 8.2 h on viscose wipes, respectively. Independently of the type of wipe, dry cleaning removed, or drastically reduced, initial spot contamination of hNoV on surfaces. All wipes transferred hNoV to an uncontaminated carrier; however, the risk of continued transmission by reuse of wipes after 6 and 24 h was limited for all viruses. We conclude that cleaning wet spots with dry wipes efficiently reduced spot contamination on surfaces but that cross-contamination with noroviruses by wiping may result in an increased public health risk at high initial virus loads. For influenza virus, IPS wipes present an efficient one-step procedure for cleaning and disinfecting contaminated surfaces.

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“…17,18 After phototreatment, both viruses showed cross-linking of viral membrane proteins, which are involved in this fusion. Although the use of uroporphyrin for virus inactivation has never been reported, the photodynamic effects of porphyrins, including uroporphyrin, on isolated cells, cell membranes, subcellular organelles and on proteins of cytosol and plasma have been investigated extensively.…”

Section: Discussionmentioning

confidence: 98%