Photoluminescence as a Complementary Tool for UV-VIS Spectroscopy to Highlight the Photodegradation of Drugs: A Case Study on Melatonin

Daescu, Monica; Toulbe, N'ghaya; Baibarac, M.; Mogos, Alin; Lőrinczi, Á.; Logofatu, C.

doi:10.3390/molecules25173820

Cited by 7 publications

(3 citation statements)

References 25 publications

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“…Mel release behavior tests were carried out three times with a UV-Spectrophotometer (Shimadzu, JAPAN) at a wavelength of 220 nm at 37°C and standard solutions were prepared at 0.5, 1, 2, 5, and 10 mg/L, respectively. 30 To maintain the sink conditions, PBS was replaced with the same volume.…”

Section: In Vitro Experimentsmentioning

confidence: 99%

Development of melatonin-embedded PLGA-PEG6000 nanofiber biomaterial, and investigation of the effects on abdominal adhesion formation

Gökçe,

Aykora,

Danışman

et al. 2023

Journal of Bioactive and Compatible Polymers

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Abdominal adhesions are still among the most common postsurgical peritoneal inflammation-related complications. Adhesion-related disorders are still highly costly and prevalent due to advances in surgical techniques, treatment methods, and various drugs. The present study aimed to investigate the effects of Poly ( D,L-lactide-co-glycolide) (PLGA)- polyethylene glycol (PEG6000) Nanofiber + Melatonin on the abdominal adhesion model in rats. For this purpose, PLGA-PEG6000 Nanofiber + Melatonin matrix was fabricated and implanted in an experimental abdominal adhesion model in rats. Our study consisted of an in vitro and an in vivo part. The degradation and release profile of the matrix and Melatonin (Mel) embedded matrix was performed in vitro. In vivo, the procedure was carried out with 18 Wistar male rats. Rats were divided into three groups as follows: Sham, Matrix, and Mel + Matrix, respectively. Consequent to degradation and release profiling in vitro, an experimental adhesion model was created and fabricated pure matrix (2 × 2 cm2), and matrix (2 × 2 cm2, 0.25 mg melatonin/per matrix embedded) was applied to injury area in related groups. Intra-abdominal adhesion scores were determined on post-op 21st day, under general anesthesia. Following, cecum, peritoneal tissue, and adhesive bands were harvested. Macroscopic analysis (severity of adhesion formation), Hematoxlyn&Eosin and Masson’s Trichrome staining (for the examination of the levels of infiltration of inflammatory cells, fibrosis, and neovascularization) were performed for the evaluation of the effects of Mel embedded and pure matrix Our results indicated that PLGA-PEG6000 Nanofiber + Melatonin matrix was degraded completely in rats abdominal cavity and significantly reduced adhesion formation compared to other groups macroscopically ( p < 0.05). On the other hand, the histopathological analysis indicated that the fabricated matrix reduced inflammatory cell infiltration, fibrosis, and neovascularization levels.

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Section: In Vitro Experimentsmentioning

confidence: 99%

Development of melatonin-embedded PLGA-PEG6000 nanofiber biomaterial, and investigation of the effects on abdominal adhesion formation

Gökçe,

Aykora,

Danışman

et al. 2023

Journal of Bioactive and Compatible Polymers

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“…Arguments for the formation of reaction compounds were often reported by Raman scattering and IR spectroscopy [ 35 , 36 , 37 , 38 ]. In order to exemplify this progress, the following issues were reported in previous studies by our team [ 35 , 36 , 37 , 38 ]: (1) the influence of oxygen from air on the photodegradation process of azathioprine in the powder and tablet state, respectively [ 38 ]; (2) the influence of the phosphate buffer (PB) solution with pH equal to 5.4 on photodegradation of folic acid [ 37 ]; and (3) the photodegradation induced by the hydrolysis reaction of melatonin [ 36 ] and acetaminophen [ 35 ]. In all these studies, PL was found to be a faster optical method in highlighting the photodegradation processes of the above drugs in comparison with the UV-VIS spectroscopy [ 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

The Influence of UV Light on Photodegradation of Acetylsalicylic Acid

Daescu

Iota

Serbschi³

et al. 2021

IJMS

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Photodegradation of the aqueous solutions of acetylsalicylic acid, in the absence (ASA) and the presence of excipients (ASE), is demonstrated by the photoluminescence (PL). A shift of the PL bands from 342 and 338 nm to 358 and 361–397 nm for ASA and ASE in solid state and as aqueous solutions was reported. By exposure of the solution of ASA 0.3 M to UV light, a decrease in the PL band intensity was highlighted. This behavior was revealed for ASA in the presence of phosphate buffer (PB) having the pH equal to 6.4, 7, and 8 or by the interaction with NaOH 0.3 M. A different behavior was reported in the case of ASE. In the presence of PB, an increase in the intensity of the PL band of ASE simultaneously with a change of the ratio between the intensities of the bands at 361–364 and 394–397 nm was highlighted. The differences between PL spectra of ASA and ASE have their origin in the presence of salicylic acid (SAL). The interaction of ASE with NaOH induces a shift of the PL band at 405–407 nm. Arguments for the reaction of ASA with NaOH are shown by Raman scattering and FTIR spectroscopy.

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“…Generally speaking, for the drugs photodegradation monitoring, the optical methods often used were UV–VIS spectroscopy, XPS spectroscopy and photoluminescence (PL) 21 – 24 . Recently, using these three methods it has been demonstrated that: (1) azathioprine photodegrades in the presence of oxygen from air 21 ; (2) the photodegradation process of folic acid in phosphate buffer (PB) with pH equal to 5.4 is enhanced 22 ; and (3) melatonin and acetaminophen, respectively, also are susceptible for photodegradation in the presence of alkaline medium 23 , 24 .…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic studies on photodegradation of atorvastatin calcium

Oprica

Iota

Daescu

et al. 2021

Sci Rep

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In this work, the photodegradation process of atorvastatin calcium (ATC) is reported as depending on: (1) the presence and the absence of excipients in the solid state; (2) the chemical interaction of ATC with phosphate buffer (PB) having pH equal to 7 and 8; and (3) hydrolysis reaction of ATC in the presence of aqueous solution of NaOH. The novelty of this work consists in the monitoring of the ATC photodegradation by photoluminescence (PL). The exposure of ATC in solid state to UV light induces the photo-oxygenation reactions in the presence of water vapors and oxygen from air. According to the X-ray photoelectron spectroscopic studies, we demonstrate that the photo-oxygenation reaction leads to photodegradation compounds having a high share of C=O bonds compared to ATC before exposure to UV light. Both in the presence of PB and NaOH, the photodegradation process of ATC is highlighted by a significant decrease in the intensity of the PL and photoluminescence excitation (PLE) spectra. According to PLE spectra, the exposure of ATC in the presence of NaOH to UV light leads to the appearance of a new band in the spectral range 340–370 nm, this belonging to the photodegradation products. Arguments concerning the chemical compounds, that resulted in this last case, are shown by Raman scattering and FTIR spectroscopy.

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Photoluminescence as a Complementary Tool for UV-VIS Spectroscopy to Highlight the Photodegradation of Drugs: A Case Study on Melatonin

Cited by 7 publications

References 25 publications

Development of melatonin-embedded PLGA-PEG6000 nanofiber biomaterial, and investigation of the effects on abdominal adhesion formation

Development of melatonin-embedded PLGA-PEG6000 nanofiber biomaterial, and investigation of the effects on abdominal adhesion formation

The Influence of UV Light on Photodegradation of Acetylsalicylic Acid

Spectroscopic studies on photodegradation of atorvastatin calcium

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