Photophysical properties and fluorescence lifetime imaging of exfoliated near-infrared fluorescent silicate nanosheets

Selvaggio, Gabriele; Weitzel, Milan; Oleksiievets, Nazar; Oswald, Tabea A.; Nißler, Robert; Mey, Ingo; Karius, Volker; Enderlein, Jörg; Tsukanov, Roman; Kruss, Sebastian

doi:10.1039/d1na00238d

Cited by 21 publications

(47 citation statements)

References 60 publications

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“…An example for remote imaging are NIR fluorescent nanosheets, which were remotely detected in a customized portable setup [171, 188] . Due to the high brightness and emission closer to the visible range (910 nm), a low‐cost CMOS camera (Complementary Metal Oxide Semiconductor, Si based), which typically has a low quantum efficiency of around 5 % in the NIR region, could be used here.…”

Section: Swcnt‐based Sensorsmentioning

confidence: 99%

Biosensing with Fluorescent Carbon Nanotubes

et al. 2022

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Biosensors are powerful tools for modern basic research and biomedical diagnostics. Their development requires substantial input from the chemical sciences. Sensors or probes with an optical readout, such as fluorescence, offer rapid, minimally invasive sensing of analytes with high spatial and temporal resolution. The near‐infrared (NIR) region is beneficial because of the reduced background and scattering of biological samples (tissue transparency window) in this range. In this context, single‐walled carbon nanotubes (SWCNTs) have emerged as versatile NIR fluorescent building blocks for biosensors. Here, we provide an overview of advances in SWCNT‐based NIR fluorescent molecular sensors. We focus on chemical design strategies for diverse analytes and summarize insights into the photophysics and molecular recognition. Furthermore, different application areas are discussed—from chemical imaging of cellular systems and diagnostics to in vivo applications and perspectives for the future.

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Section: Swcnt‐based Sensorsmentioning

confidence: 99%

Biosensing with Fluorescent Carbon Nanotubes

et al. 2022

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“…neurotransmitters 15,16 ), their established separation 10,17 and functionalization routes 8,18 , as well as the possibility to perform defect chemistry [19][20][21] . 2D materials such as exfoliated silicate nanosheets (NS), on the other side, stand out for their high fluorescence brightness and remarkable photostability, which make them very promising labelling agents for multiple bioimaging scenarios 22,23 . Organic fluorophores tend to have shorter emission wavelengths and limited solubility in water but they also display faster excretion kinetics and can be chemically tailored, which makes them versatile materials for bioimaging 6 .…”

Section: Introductionmentioning

confidence: 99%

NIR-emitting benzene-fused oligo-BODIPYs for bioimaging

Selvaggio¹,

Nißler²,

Nietmann³

et al. 2022

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“…This calcium copper tetrasilicate displays a layered crystal structure which allows to prepare nanosheets (NS) via simple stirring in hot water [22][23][24][25][26][27] . Most interestingly, it has been shown that the bright, stable and long-lived nearinfrared (NIR) fluorescence of the bulk material (λem ≈ 910-930 nm) [25][26][27][28][29][30] is preserved in exfoliated NS down to just few tenths of nm in size 31,32 . The biocompatible nature of this class of layered silicates has been demonstrated by cell viability assays and in vivo studies 31 .…”

Section: Introductionmentioning

confidence: 99%

“…The biocompatible nature of this class of layered silicates has been demonstrated by cell viability assays and in vivo studies 31 . Among them, an increasing number of publications has proven the high potential of EB-NS in the biomedical field as multi-functional platform for bioimaging 31,32 , sensing 33 , photothermal therapy (PTT) 34,35 , tissue engineering 36,37 and more. Especially as novel NIR fluorophore, EB-NS appears of extreme interest.…”

Section: Introductionmentioning

confidence: 99%

“…For these reasons, the interest in novel NIR materials is very high. Although the potential of EB-NS as NIR fluorophore has already been recognized 31,32 , the next important step in this direction is tailoring of its surface chemistry. So far, the few publications which have described functionalization procedures on EB-NS have focused on lipophilic coatings.…”

Section: Introductionmentioning

confidence: 99%

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Covalently Functionalized Egyptian Blue Nanosheets for Near-Infrared Bioimaging

Selvaggio

Herrmann

Hill

et al. 2022

Preprint

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Fluorophores emitting in the near-infrared (NIR) wavelength region present optimal characteristics for photonics and especially bioimaging. Unfortunately, only few NIR fluorescent materials are known and even fewer are biocompatible. For this reason, the scientific interest in designing novel NIR fluorophores is very high. Egyptian Blue (CaCuSi4O10, EB) is a NIR fluorescent layered silicate that can be exfoliated into fluorescent nanosheets (EB-NS). So far, its surface chemistry has not been tailored but this is crucial for colloidal stability and biological targeting. Here, we demonstrate covalent surface functionalization of EB nanosheets (EBfunc) via Si-H activation using hydrosilanes with variable functionalities. EB-NS were first grafted with the visible fluorescent pyrene (Pyr) moieties to prove conjugation by colocalization of the Vis/NIR fluorescence on the (single) EB-NS level. The same procedure was performed and validated with carboxyl group (COOH)-containing hydrosilanes. These groups can serve as generic handle for further (bio)functionalization of the EB-NS surface. Finally, folic acid (FA) was conjugated to these COOH-functionalized EB-NS to target folic acid receptor-expressing cancer cells. These results highlight the potential of this surface chemistry approach to modify EB-NS and enable targeted NIR imaging for biomedical applications.

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Photophysical properties and fluorescence lifetime imaging of exfoliated near-infrared fluorescent silicate nanosheets

Abstract: The layered silicates Egyptian Blue (CaCuSi4O10, EB), Han Blue (BaCuSi4O10, HB) and Han Purple (BaCuSi2O6, HP) emit as bulk material bright and stable fluorescence in the near-infrared (NIR), which is...

Cited by 21 publications

References 60 publications

Biosensing with Fluorescent Carbon Nanotubes

Biosensing with Fluorescent Carbon Nanotubes

NIR-emitting benzene-fused oligo-BODIPYs for bioimaging

Covalently Functionalized Egyptian Blue Nanosheets for Near-Infrared Bioimaging

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