Photosynthetic Performance of Emiliania Huxleyi in a Fluctuating Light Climate Simulating Vertical Mixing Montepellier, France, 20–25 August 1995

Kromkamp, Jacco C.; Peene, J.; Flameling, Inez A.

doi:10.1007/978-94-009-0173-5_1096

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…However, algae, especially chl c containing microalgae, show a lower optimal Fv/Fm (Ting & Owens 1992, Ibelings et al 1994, Flameling & Kromkamp 1995, Kromkamp et al 1995b; see Biichel & Wilhelm 1993 for a review on algal fluorescence). We therefore conclude from the F,JFm ratio that the PSII reaction centres were fully functional and that nutrient limitation by N, P or Si did not occur.…”

Section: Discussionmentioning

confidence: 99%

Determination of microphytobenthos PSII quantum efficiency and photosynthetic activity by means of variable chlorophyll fluorescence

Kromkamp¹,

Barranguet²,

Peene³

1998

Mar. Ecol. Prog. Ser.

205

184

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A pulse amplitude modulated fluorometer (PAM) was used to investigate photosynthetic activity of m~crophytobenthos on an Intertidal mudflat Spectral ~rradiance measurements Indicate that 7500 of the signal detectable by the PAM onginates In the upper 150 pm of the s e d~m e n t From the photosynthetic electron transport rate (ETR) measurements, it was concluded that the PAM could be used to observe changes in photosynthetic parameters during the day or the season Photoacclimation to lower irrad~ance was ind~cated by changes in the maximum ETR and the saturating photon irradiance parameter I, When cores were exposed to a high photon irradiance for several hours, vertical migration could be followed using reflectance spectra The data also showed that the benthic algae did not seem to expenence photoinhib~tion or COZ limitation To explain this, ~t is hypothes~sed that there is a continuous vertical migration in the top layer of the s e d~m e n t , whele algae can a v o~d photoinhibition due to prolonged periods of high irrad~ance and lack of CO2 by m~grating downwards whlle others migrate upwards KEY WORDS Microphytobenthos Chlorophyll fluorescence Photosynthesis Vert~cal I-rugratlon C-limitation

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Section: Discussionmentioning

confidence: 99%

Determination of microphytobenthos PSII quantum efficiency and photosynthetic activity by means of variable chlorophyll fluorescence

Kromkamp¹,

Barranguet²,

Peene³

1998

Mar. Ecol. Prog. Ser.

205

184

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“…The major drawback is that they measure the rate of PSII photosynthetic electron transport (ETR) instead of C-fixation, which is implicitly assumed to be the benchmark against which all other techniques are compared. However, a good relationship between ETR and C-fixation or oxygen evolution has been observed in both higher plants (Genty et al, 1989) and microalgal culture studies (Kromkamp et al, 1995;Gilbert et al, 2000;Sugget et al 2003), and in FRRFbased field studies Suggett et al, 2001Suggett et al, , 2006Moore et al, 2003;Raateoja et al, 2004a;Smyth et al, 2004;Corno et al, 2006;Melrose et al, 2006).…”

Section: Introductionmentioning

confidence: 90%

Estimating phytoplankton primary production in Lake IJsselmeer (The Netherlands) using variable fluorescence (PAM-FRRF) and C-uptake techniques

Kromkamp

Dijkman

Peene

et al. 2008

European Journal of Phycology

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In this paper we compare measured rates of C-fixation obtained using in situ deployment of a fast repetition rate fluorometer (FRRF) applying two different calculation protocols and a waterPAM fluorometer. The measurements were carried out in the large, shallow Lake IJsselmeer (The Netherlands). Turbidity appears to be the most important factor in explaining photosynthetic parameters. Non-photochemical quenching (NPQ) in the antenna was observed, and the degree of NPQ was positively correlated with the irradiance and mixing conditions. However, NPQ did not affect the functional cross-section. Conversion of photosynthetic electron transport rates (ETR) into rates of carbon fixation requires knowledge of two or three parameters (depending on the protocol and instrument used) to calculate C-fixation from ETR: the electron yield (È e , i.e. the reciprocal of the quantum requirement of PSII), the photosynthetic quotient (PQ) and the number of PSII (n PSII ). Using normally assumed values for these three parameters resulted in overestimation of the measured rate of C-fixation but, in all cases, ETR was linearly related to it. The degree of overestimation was rather constant, despite considerable changes in phytoplankton composition. Spectral correction of fluorescence data caused a reduction of 30% or an increase of 44% in the estimated C-fixation, depending how C-fixation was estimated. We describe a method that allows estimation of the È e /PQ ratio based on a comparison of the quantum efficiencies for C-fixation and PSII and show that, with a single and realistic value for this ratio, primary production could be accurately predicted at different times of the year.

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“…High rates of nitrogenase activity during the morning, followed by a decrease in rates during the afternoon, have been observed on several occasions in both pelagic and benthic systems (Vanderhoef et al, 1975 ;Carpenter et al, 1978). A midday drop is also often seen in photosynthesis, and this has been attributed to a decrease in quantum efficiency (Kromkamp et al, 1995). The physiological basis for such a decrease in quantum efficiency is not yet known but, as a decrease in quantum efficiency is equivalent to a decrease in photon flux density, this phenomenon can either stimulate or inhibit N # fixation depending upon whether the prevailing photon flux density is suboptimal or superoptimal for N # fixation (Fig.…”

Section: mentioning

confidence: 99%

Nitrogen fixation by Baltic cyanobacteria is adapted to the prevailing photon flux density

et al. 2000

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N # fixation, measured as acetylene reduction, was studied in laboratory cultures and in natural assemblages (both as a mixed population and as individually picked colonies) of the heterocystous cyanobacteria Aphanizomenon sp. and Nodularia spp. from the Baltic Sea. During a diurnal cycle of alternating light and darkness, these organisms reduced acetylene predominantly during the period of illumination, although considerable activity was also observed during the dark period. In both laboratory cultures and natural populations N # fixation was saturated below a photon flux density of 600 µm −# s −" . In cyanobacterial blooms in the Baltic Sea, nitrogenase activity was mostly confined to the surface layers. Samples collected from greater depths did not possess the same capacity for acetylene reduction as samples from the surface itself, even when incubated at the photon flux density prevailing in surface waters. This suggests that, with respect to N # fixation, Baltic cyanobacteria are adapted to the intensity of illumination that they are currently experiencing.

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Photosynthetic Performance of Emiliania Huxleyi in a Fluctuating Light Climate Simulating Vertical Mixing Montepellier, France, 20–25 August 1995

Cited by 3 publications

References 2 publications

Determination of microphytobenthos PSII quantum efficiency and photosynthetic activity by means of variable chlorophyll fluorescence

Determination of microphytobenthos PSII quantum efficiency and photosynthetic activity by means of variable chlorophyll fluorescence

Estimating phytoplankton primary production in Lake IJsselmeer (The Netherlands) using variable fluorescence (PAM-FRRF) and C-uptake techniques

Nitrogen fixation by Baltic cyanobacteria is adapted to the prevailing photon flux density

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