Phototherapy and optical waveguides for the treatment of infection

Wang, Dingbowen; Kuzma, Michelle; Tan, Xinyu; He, Tong‐Chuan; Dong, Chuan; Liu, Zhiwen; Yang, Jian

doi:10.1016/j.addr.2021.114036

Cited by 41 publications

(25 citation statements)

References 681 publications

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“…The limited penetration of aBL in the human tissues, coupled with the invasiveness of V. vulnificus , may render the treatment of more established V. vulnificus infections difficult. Therefore, the use of optical clearing agents and/or microneedle arrays for the interstitial administration of aBL may be required to ensure improved exposure of the invading bacteria to aBL (Kim et al, 2016 ; Wang et al, 2021 ; Tuchin et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Blue Light for Prevention and Treatment of Highly Invasive Vibrio vulnificus Burn Infection in Mice

et al. 2022

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Vibrio vulnificus is an invasive marine bacterium that causes a variety of serious infectious diseases. With the increasing multidrug-resistant variants, treatment of V. vulnificus infections is becoming more difficult. In this study, we explored antimicrobial blue light (aBL; 405 nm wavelength) for the treatment of V. vulnificus infections. We first assessed the efficacy of aBL against five strains of V. vulnificus in vitro. Next, we identified and quantified intracellular porphyrins in V. vulnificus to provide mechanistic insights. Additionally, we measured intracellular reactive oxygen species (ROS) production and bacterial membrane permeabilization following aBL exposures. Lastly, we conducted a preclinical study to investigate the efficacy and safety of aBL for the prevention and treatment of burn infections caused by V. vulnificus in mice. We found that aBL effectively killed V. vulnificus in vitro in both planktonic and biofilm states, with up to a 5.17- and 4.57-log10 CFU reduction being achieved, respectively, following an aBL exposure of 216 J/cm2. Protoporphyrin IX and coproporphyrins were predominant in all the strains. Additionally, intracellular ROS was significantly increased following aBL exposures (P < 0.01), and there was evidence of aBL-induced permeabilization of the bacterial membrane (P < 0.0001). In the preclinical studies, we found that female mice treated with aBL 30 min after bacterial inoculation showed a survival rate of 81% following 7 days of observation, while only 28% survival was observed in untreated female mice (P < 0.001). At 6 h post-inoculation, an 86% survival was achieved in aBL-treated female mice (P = 0.0002). For male mice, 86 and 63% survival rates were achieved when aBL treatment was given 30 min and 6 h after bacterial inoculation, respectively, compared to 32% survival in the untreated mice (P = 0.0004 and P = 0.04). aBL did not reduce cellular proliferation or induce apoptosis. We found five cytokines were significantly upregulated in the males after aBL treatment, including MCSF (P < 0.001), MCP-5 (P < 0.01), TNF RII (P < 0.01), CXCL1 (P < 0.01), and TIMP-1 (P < 0.05), and one in the females (TIMP-1; P < 0.05), suggesting that aBL may induce certain inflammatory processes. In conclusion, aBL may potentially be applied to prevent and treat V. vulnificus infections.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Blue Light for Prevention and Treatment of Highly Invasive Vibrio vulnificus Burn Infection in Mice

et al. 2022

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“…[ 5 ] Currently, near‐infrared (NIR) light‐activatable strategies have attracted wide attention due to the advantages of spatiotemporal controllability, improved penetration depth (>3 mm) compared to visible light, minimal photodamage, and avoidance of bacterial resistance. [ 6 ] Especially, photothermal (PTT)‐derived multimodal synergistic antibacterial therapy is widely acceptable strategy. [ 7 ] It has been reported that diverse NIR‐responsive nanomaterials were integrated into the implant surface for the construction of photo‐triggered bactericidal surfaces.…”

Section: Introductionmentioning

confidence: 99%

Polydopamine‐Mediated Interfacial Functionalization of Implants for Accelerating Infected Bone Repair through Light‐Activatable Antibiosis and Carbon Monoxide Gas Regulated Macrophage Polarization

Yuan

et al. 2022

Adv Funct Materials

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Current treatments for implant-associated infection remain unsatisfactory due to secondary infection and excessive inflammation, which impairs osseointegration. Herein, an interfacial functionalization strategy is proposed by the integration of a carbon monoxide gas (CO) nanogenerator on titanium implants, followed by covalently grafting arginine-glycine-aspartic acid (RGD) polypeptide. Under near-infrared light (NIR) irradiation, the designed surface displays great light-activatable antibiosis through CO-potentiated mild photothermal therapy. Interestingly, the functionalized surface exerts a CO-mediated anti-inflammatory effect by activating the expression of heme oxygenase (HO-1), and inducing the down-regulation of p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NFκB) (p50/p65). More importantly, the combination of CO delivery and RGD immobilization drives the polarization of lipopolysaccharide (LPS)-stimulated M1-phenotype macrophages towards anti-inflammatory M2-pheno type through a potentialJanus kinase 1/signal transducer and activator of transcription 6 (JAK1/STAT6) pathway, thereby remodeling the damaged microenvironment into a proregenerative microenvironment. In a rat model of implant-associated infection, the designed surface effectively eliminates the residual bacteria, alleviates the accompanying inflammation and mediates macrophagemediated immunomodulation, resulting in good osteogenesis. Together, these findings are a first report on the therapeutic potential of CO signal in the cascade of immunomodulation-osteogenic differentiation. The functionalized implant may serve as a promising candidate in implant replacement surgeries.

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“…For instance, the light employed for photothermal agents always shows weak tissue penetration. It also always possesses side effects on normal tissues and low efficiencies [ 102 , 103 ]. In order to overcome these shortcomings, a supramolecular self-assembly strategy has been employed to improve the therapeutic performance of PTT in two ways, including improving the photothermal conversion efficiency and regulating the interaction between PTT systems and bacteria.…”

Section: Supramolecular Self-assembly For Improvement Of Pttmentioning

confidence: 99%

Combatting Antibiotic Resistance Using Supramolecular Assemblies

Guo

Yuan-yuan

et al. 2022

Pharmaceuticals

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Antibiotic resistance has posed a great threat to human health. The emergence of antibiotic resistance has always outpaced the development of new antibiotics, and the investment in the development of new antibiotics is diminishing. Supramolecular self-assembly of the conventional antibacterial agents has been proved to be a promising and versatile strategy to tackle the serious problem of antibiotic resistance. In this review, the recent development of antibacterial agents based on supramolecular self-assembly strategies will be introduced.

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Phototherapy and optical waveguides for the treatment of infection

Cited by 41 publications

References 681 publications

Antimicrobial Blue Light for Prevention and Treatment of Highly Invasive Vibrio vulnificus Burn Infection in Mice

Antimicrobial Blue Light for Prevention and Treatment of Highly Invasive Vibrio vulnificus Burn Infection in Mice

Polydopamine‐Mediated Interfacial Functionalization of Implants for Accelerating Infected Bone Repair through Light‐Activatable Antibiosis and Carbon Monoxide Gas Regulated Macrophage Polarization

Combatting Antibiotic Resistance Using Supramolecular Assemblies

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