Phthalates affect the in vitro expansion of human hematopoietic stem cell

Gutiérrez-García, Ana K.; Flores-Kelly, José M; Ortiz-Rodríguez, Tomás; Kalixto-Sánchez, Marco A.; León‐Rodríguez, Antonio De

doi:10.1007/s10616-019-00300-x

Cited by 18 publications

(9 citation statements)

References 59 publications

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“…MEHP in a high concentration (1,000 µmol/L or 164,110 ng/mL) decreased the expression of genes related to mesoderm and the primary germ cells, induced cytotoxicity, and reduced cell proliferation and viability (Shi et al, 2013). Studies examining the effects on in vitro expansion of human hematopoietic cells from umbilical cord blood found that four phthalates-DBP, benzyl butyl phthalate (BBP), DEP, and DEHP decreased the cell expansion with DBP being the most cytotoxic (Gutierrez-Garcia et al, 2019).…”

Section: Effect Of Phthalates On Different Tissues Embryonic Stem Celmentioning

confidence: 99%

“…Phthalates can act as endocrine disrupting chemicals (EDCs) by exerting strong antiandrogenic (Doyle et al, 2013;Martino-Andrade et al, 2016) and weak estrogenic (Lee et al, 2012;Huang P. C. et al, 2014) effects. Though, EDC exposure can be harmful at any stage of human life, the developing human fetus in a phase of rapid proliferative growth in utero may be particularly vulnerable (Gutierrez-Garcia et al, 2019). The in utero environment or the preconception time period has been regarded as the most vulnerable period of growth and development to environmental insults (Chapin et al, 2004).…”

Section: Effect Of Phthalates As Endocrine Disrupting Chemicals On Emmentioning

confidence: 99%

“…The persistence of phthalates in the environment makes them an emerging public health concern, as they have potential effects on reproduction, development, obesity, and other public health problems. In animals, phthalates are lipid soluble enabling easy storage in adipose tissue for long periods (Gutierrez-Garcia et al, 2019). In this review, we document several studies that suggest that phthalate exposure in humans, at real-world levels, might have some toxicological risk, with a focus on epigenetic changes associated with phthalate exposures.…”

Section: Introductionmentioning

confidence: 99%

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Phthalate Exposure and Long-Term Epigenomic Consequences: A Review

et al. 2020

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Phthalates are esters of phthalic acid which are used in cosmetics and other daily personal care products. They are also used in polyvinyl chloride (PVC) plastics to increase durability and plasticity. Phthalates are not present in plastics by covalent bonds and thus can easily leach into the environment and enter the human body by dermal absorption, ingestion, or inhalation. Several in vitro and in vivo studies suggest that phthalates can act as endocrine disruptors and cause moderate reproductive and developmental toxicities. Furthermore, phthalates can pass through the placental barrier and affect the developing fetus. Thus, phthalates have ubiquitous presence in food and environment with potential adverse health effects in humans. This review focusses on studies conducted in the field of toxicogenomics of phthalates and discusses possible transgenerational and multigenerational effects caused by phthalate exposure during any point of the life-cycle.

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Section: Effect Of Phthalates On Different Tissues Embryonic Stem Celmentioning

confidence: 99%

Section: Effect Of Phthalates As Endocrine Disrupting Chemicals On Emmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phthalate Exposure and Long-Term Epigenomic Consequences: A Review

et al. 2020

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“…However, further investigations are required to determine if DEHP exposure in uences other physiological processes. According to a recent study, DEHP affects the function of human haematopoietic stem and progenitor cells (HSPCs) isolated from human umbilical cord blood [5]. DEHP also impairs the migration and colony-forming capacity of human CD34 + HSPCs [6].…”

Section: Introductionmentioning

confidence: 99%

Di(2-ethylhexyl)phthalate Impairs Erythropoiesis via Inducing Klotho Expression and Not via Bioenergetic Reprogramming

Tsai¹,

Fang²,

Chen³

et al. 2020

Preprint

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Background: Di(2-ethylhexyl)phthalate (DEHP) is one of the phthalates most widely used to manufacture various plastic products. However, the potential effects of DEHP on erythropoiesis have not been investigated comprehensively. Here, we aimed to investigate whether DEHP modulate the function of haematopoietic stem and progenitor cells (HSPCs) to influence erythropoiesis, and to explore the associated mechanisms.Results: In the present study, human cell lines with a capacity to differentiate into erythroid cells and murine bone marrow cells were treated with DEHP. DEHP not only impaired HSPC function but also suppressed erythroid differentiation in a dose-dependent manner. In addition, DEHP removal restored HSPC activity. To explore how DEHP interferes with erythroid differentiation, we focused on energy metabolism and Klotho expression. DEHP suppressed erythroid differentiation via upregulation of Klotho expression in erythroblasts and not via cellular bioenergetic modulation.Conclusions: Here, we have demonstrated a novel pathophysiological role of DEHP in erythroid differentiation. DEHP impaired erythroid differentiation by inducing Klotho expression and not by reprogramming cellular bioenergetic profiles during differentiation.

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“…We focus on the effects of environmental stressors that impact these operations. We also examine cell viability to analyze toxicity and provide a fuller picture of a toxicant’s effect, by correlating cell number to cell behavior[6,7].…”

Section: Introductionmentioning

confidence: 99%

Effects of environmental stressors on stem cells

Worley

Parker

2019

WJSC

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Environmental toxicants are ubiquitous, and many are known to cause harmful health effects. However, much of what we know or think we know concerning the targets and long-term effects of exposure to environmental stressors is sadly lacking. Toxicant exposure may have health effects that are currently mischaracterized or at least mechanistically incompletely understood. While much of the recent excitement about stem cells (SCs) focuses on their potential as therapeutic agents, they also offer a valuable resource to give us insight into the mechanisms and risks of toxicant effects. Not only as a response to the increasing ethical pressure to reduce animal testing, SC studies allow us valuable insight into the true effects of human exposure to environmental stressors under controlled conditions. We present a review of the history of publications on the effects of environmental stressors on SCs, followed by a consolidation of the literature over the past five years on a subset of key environmental stressors of importance to human health and their effects on both embryonic and tissue SCs. The review will make constructive suggestions as to areas of toxicant research where further studies are needed, as well as making indications of the potential utility for advancing knowledge and directing research on environmental toxicology.

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Phthalates affect the in vitro expansion of human hematopoietic stem cell

Cited by 18 publications

References 59 publications

Phthalate Exposure and Long-Term Epigenomic Consequences: A Review

Phthalate Exposure and Long-Term Epigenomic Consequences: A Review

Di(2-ethylhexyl)phthalate Impairs Erythropoiesis via Inducing Klotho Expression and Not via Bioenergetic Reprogramming

Effects of environmental stressors on stem cells

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