Phycocyanin is an Antioxidant Protector of Human Erythrocytes Against Lysis by Peroxyl Radicals

Romay, Cheyla; González, Ricardo

doi:10.1211/0022357001774093

Cited by 110 publications

(72 citation statements)

References 9 publications

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“…SP partially prevented these alterations induced by GS and this prevention could be associated with the ability of SP to partially ameliorate tubular necrosis. It has been established that phycocyanin not only scavenges peroxyl, hydroxyl (Vadiraja et al, 1998), peroxynitrite (Bhat and Madyastha, 2000), but also acts as a potent antioxidant and inhibits lipid peroxidation (Romay and Gonzalez, 2000). Mice pretreated with SP, significantly attenuated the cyclophosphamide and mitomycin-C-induced decrease in SOD, catalase, glutathione and glutathione dependent enzymes in the liver (Premkumar et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Spirulina platensis protects against gentamicin‐induced nephrotoxicity in rats

Karadeniz

Yıldırım

Şimşek

et al. 2008

Phytotherapy Research

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The present study aimed to investigate the protective effect of Spirulina platensis (SP) on gentamicin sulphate (GS)-induced changes in the levels of lipid peroxidation and endogenous antioxidants in the kidney of rats. Sprague-Dawley rats were treated in separate groups as follows for 7 consecutive days: control (C), gentamicin sulphate (100 mg/kg i.p.) (GS), Spirulina platensis (1000 mg/kg orally) (SP) and Spirulina platensis (1000 mg/kg orally) plus gentamicin sulphate (100 mg/kg i.p.) (SP + GS). The degree of protection was evaluated by determining the effects of Spirulina platensis on malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPX) and nitric oxide (NO), and plasma creatinine and urea levels were estimated in kidney homogenates to evaluate antioxidant activity, and the kidney was histologically examined as well. Spirulina platensis elicited significant nephroprotective activity by decreasing lipid peroxidation (MDA) and elevated the levels of GSH, SOD, GPX, NO, creatinine and urea. Furthermore, these biochemical observations were supplemented by histological examination of the rat kidneys. In conclusion, the present study indicates a very important role of reactive oxygen species (ROS) and the relation to renal dysfunction and point to the therapeutic potential of Spirulina platensis in gentamicin sulphate induced nephrotoxicity.

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Section: Discussionmentioning

confidence: 99%

Spirulina platensis protects against gentamicin‐induced nephrotoxicity in rats

Karadeniz

Yıldırım

Şimşek

et al. 2008

Phytotherapy Research

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“…Several studies have shown that Spirulina has potent antioxidant activity (Premkumar et al, 2001;Upasani and Balaraman, 2003;Khan et al, 2005), and is reported to possess hydroxyl and peroxyl radical scavenging activity both in vitro and in vivo by phycocyanin (Bhat and Madyastha, 2000). It has been established that phycocyanin not only scavenges peroxyl, hydroxyl (Vadiraja et al, 1998), peroxynitrite (Bhat and Madhyastha, 2001) and superoxide radicals (Romay et al, 1998), but also acts as a potent antioxidant and inhibits lipid peroxidation (Romay et al, 1998;Gonzalez et al, 1999;Romay and Gonzalez, 2000;Remirez et al, 2002). Mice pretreated with Spirulina, significantly attenuated the cyclophosphamide and mitomycin-C-induced decrease in SOD, catalase, glutathione and glutathionedependent enzymes in the liver (Premkumar et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Spirulina attenuates cyclosporine‐induced nephrotoxicity in rats

Khan¹,

Shobha²,

Mohan³

et al. 2006

J of Applied Toxicology

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Cyclosporine (CsA) causes a dose-related decrease in renal function in experimental animals and humans. The generation of reactive oxygen species (ROS) has been implicated in CsA-induced nephrotoxicity. It was previously shown that Spirulina, a blue-green algae, with antioxidant properties effectively attenuated the doxorubicin-induced cardiotoxicity in mice and cisplatin-induced nephrotoxicity in rat. The present study investigated the nephroprotective role of Spirulina against CsA-induced nephrotoxicity in rats. Spirulina (500 mg kg(-1) b.w.) was administered orally for 3 days before and 14 days concurrently with CsA (50 mg kg-1 b.w.). Rats treated with CsA showed nephrotoxicity as evidenced from a significant elevation in plasma urea, creatinine, urinary N-acetyl-beta-D-glucosaminidase (beta-NAG) and a decrease in creatinine and lithium clearance. Pretreatment with Spirulina protected the rats from CsA-induced nephrotoxicity. The CsA-induced rise in plasma urea and creatinine and the decrease in creatinine and lithium clearance were attenuated by Spirulina. There was a significant increase in plasma and kidney tissue MDA with CsA. Spirulina prevented the rise in plasma and kidney tissue MDA. Histopathology of the kidney from CsA-treated rats showed severe isometric vacuolization and widening of the interstitium. However, pretreatment with Spirulina prevented such changes, and the kidney morphology was comparable to that of the control. Spirulina treatment did not alter the blood CsA levels. These results suggest that Spirulina has a protective effect against nephrotoxicity induced by CsA. This study further supports the crucial role of the antioxidant nature of Spirulina in protecting against CsA-induced oxidative stress.

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“…Phycocyanin is one of the important natural blue colorants finding food application in manufacturing of bubble gums, milky products, jelly, ice creams and beverages. Phycocyanin is also known for its antioxidant, anti-inflammatory and hepatoprotective effects (Romay and Gonzalez 2000;Reddy et al 2000;Vadiraja and Madyastha 2000;Romay et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Single step aqueous two-phase extraction for downstream processing of C-phycocyanin from Spirulina platensis

et al. 2014

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C-phycocyanin, a natural food colorant, is gaining importance worldwide due to its several medical and pharmaceutical applications. In the present study, aqueous two-phase extraction was shown to be an attractive alternative for the downstream processing of C-phycocyanin from Spirulina platensis. By employing differential partitioning, Cphycocyanin selectively partitioned to the polymer rich (top) phase in concentrated form and contaminant proteins to the salt rich (bottom) phase. This resulted in an increase in the product purity (without losing much of the yield) in a single step without the need of multiple processing steps. Effect of process parameters such as molecular weight, tie line length, phase volume ratio, concentration of phase components on the partitioning behavior of C-phycocyanin was studied. The results were explained based on relative free volume of the phase systems. C-phycocyanin with a purity of 4.32 and yield of about 79 % was obtained at the standardized conditions.

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Phycocyanin is an Antioxidant Protector of Human Erythrocytes Against Lysis by Peroxyl Radicals

Cited by 110 publications

References 9 publications

Spirulina platensis protects against gentamicin‐induced nephrotoxicity in rats

Spirulina platensis protects against gentamicin‐induced nephrotoxicity in rats

Spirulina attenuates cyclosporine‐induced nephrotoxicity in rats

Single step aqueous two-phase extraction for downstream processing of C-phycocyanin from Spirulina platensis

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