Phyllanthus orbicularis Aqueous Extract: Cytotoxic, Genotoxic, and Antimutagenic Effects in the CHO Cell Line

Sánchez-Lamar, Ángel; Fiore, Mario; Cundari, Enrico; Ricordy, R.; Cozzi, Renata; Salvia, R. De

doi:10.1006/taap.1999.8814

Cited by 32 publications

(17 citation statements)

References 21 publications

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“…Therefore, femur bone marrow erythrocytes of S-D rats treated with P. niruri did not demonstrate genotoxicity. Similarly, another Phyllanthus species, P. orbicularis (aqueous extract), showed low cytotoxicity and non-genotoxicity (Sanchez-Lamar et al, 1999). To the best of our knowledge, this is the first study reported on the non-genotoxicity of P. niruri.…”

Section: Discussionmentioning

confidence: 83%

Genotoxicity, cytotoxicity and toxicological evaluation of whole plant extracts of the medicinal plant Phyllanthus niruri (Phyllanthaceae)

Asare¹,

Bugyei²,

Sittie³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Phyllanthus niruri is a medicinal plant (commonly known as stone breaker) found in the tropics and other parts of the world. It is known for its capacity to block the formation of calcium oxalate crystals and kidney stone formation in urolithiasis. This plant has been used to treat hyperglycemia, hypertension, pain, and mild cases of malaria. We examined the geno-, cyto-and overall toxicity of P. niruri whole plant ethanolic extract. The extract was administered as a single dose of 30 or 300 mg/kg to laboratory rats by gavage, accompanied by negative (0.9% saline) and positive (10 mg/mL N-ethyl-N-nitrosourea) controls that were injected intramuscularly 48 h after extract administration. The ratio of polychromatic (PCE)/normochromatic erythrocytes (NCE) from femur bone marrow was scored for genotoxicity. Cytotoxicity was determined using descending concentrations (0.2-0.0125 g/mL) of the extract incubated with peripheral blood mononuclear cells. Phyllanthus niruri geno-and cytotoxicity Lactate dehydrogenase release from damaged cells was determined and the CC 50 calculated. Subchronic administration of the extract at 30 or 300 mg/kg was done for 90 days to determine general toxicity. PCE:NCE (%) for the extract and negative control was 63, compared to 168 (positive control). The CC 50 was 26.3 mg/mL and hepato-renal toxicity after subchronic extract administration was nil. We conclude that ethanol extract of P. niruri is not cytotoxic or genotoxic, and is generally non-toxic on subchronic administration.

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Section: Discussionmentioning

confidence: 83%

Genotoxicity, cytotoxicity and toxicological evaluation of whole plant extracts of the medicinal plant Phyllanthus niruri (Phyllanthaceae)

Asare¹,

Bugyei²,

Sittie³

et al. 2012

Genet. Mol. Res.

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“…Besides these properties, we have reported that this plant extract also has antimutagenic properties. Thus, it protects against damage induced by hydrogen peroxide because reduction of both the number of chromosome aberrations and revertants in Chinese hamster ovary (CHO) cells and in the Salmonella assay, respectively, was found (Sánchez-Lamar et al, 1999;Ferrer et al, 2002).…”

Section: Introductionmentioning

confidence: 98%

Modulation of rat and human cytochromes P450 involved in PhIP and 4-ABP activation by an aqueous extract of Phyllanthus orbicularis

Ferrer

Cristòfol²,

Sánchez-Lamar

et al. 2004

Journal of Ethnopharmacology

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“…2 Phytotherapy has been considered as an alternative to alleviate side effects associated with synthetic drugs. 3 Bioactive compounds in plants can be developed into natural products that can be used as substitutes to synthetic drugs. Furthermore, they provide the foundation for the development of new drugs.…”

Section: Introductionmentioning

confidence: 99%

Ethanol Extracts of Selected Cyathea Species Decreased Cell Viability and Inhibited Growth in MCF 7 Cell Line Cultures

Janakiraman

Johnson

2016

Journal of Acupuncture and Meridian Studies

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Cancer is the cause of more than 6 million deaths worldwide every year. For centuries, medicinal plants have been used in the treatment of cancer. Chemotherapy, radiotherapy, surgery and acupuncture point stimulation are also used to treat cancer. The present study was intended to reveal the cytotoxic and anticancer potential of selected Cyathea species and to highlight their importance in the pharmaceutical industry for the development of cost-effective drugs. Cytotoxic studies using brine shrimp lethality bioassays and MCF 7 cell line cultures were carried out. Compared to petroleum ether, chloroform and acetone extracts, the ethanol extracts of selected Cyathea species were found to be more effective against brine shrimps. The ethanol extracts were further subjected to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assays. A decrease in cell viability and an increase in growth inhibition were observed for the MCF 7 cell line. The maximum percentage of cell inhibition was observed in Cyathea crinit, followed by Cyathea nilgirensis and Cyathea gigantea. The results of the present study suggest that Cyathea species are an effective source of cytotoxic compounds.

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Phyllanthus orbicularis Aqueous Extract: Cytotoxic, Genotoxic, and Antimutagenic Effects in the CHO Cell Line

Cited by 32 publications

References 21 publications

Genotoxicity, cytotoxicity and toxicological evaluation of whole plant extracts of the medicinal plant Phyllanthus niruri (Phyllanthaceae)

Genotoxicity, cytotoxicity and toxicological evaluation of whole plant extracts of the medicinal plant Phyllanthus niruri (Phyllanthaceae)

Modulation of rat and human cytochromes P450 involved in PhIP and 4-ABP activation by an aqueous extract of Phyllanthus orbicularis

Ethanol Extracts of Selected Cyathea Species Decreased Cell Viability and Inhibited Growth in MCF 7 Cell Line Cultures

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