Phylogenetic analysis of New Zealand earthworms (Oligochaeta: Megascolecidae) reveals ancient clades and cryptic taxonomic diversity

Buckley, Thomas R.; James, S. W.; Allwood, Julia S.; Bartlam, Scott; Howitt, Robyn; Prada, Diana

doi:10.1016/j.ympev.2010.09.024

Cited by 72 publications

(68 citation statements)

References 77 publications

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“…Therefore, our work confirms previous suggestions that many New Zealand native earthworm species are yet to be described (Boyer et al 2011;Buckley et al 2011). Many New Zealand native earthworms have restricted distributions.…”

Section: Specimen Identificationsupporting

confidence: 92%

“…Sequences were manually edited using FinchTV 1.40 (Geospiza), and compared to existing sequences in the Genbank database as well as sequences from Buckley et al (2011) and. The sequences generated here and their best match in the existing databases were exported into MEGA6 (Tamura et al 2013) and Geneious® 6.1.8 (Biomatters) for alignment using MUSCLE (Edgar 2004).…”

Section: Delineation Of Molecular Taxonomic Units (Motus)mentioning

confidence: 99%

“…Neighbour joining tree for COI (left) and 16S (right) based on 36 earthworm specimens collected as part of this study (names in bold), along with their closest matches according to a MegaBLAST search against the Genbank library and sequences from and Buckley et al (2011). The enchytraeid species Friderica magna was used as an outgroup.…”

Section: Specimen Identificationmentioning

confidence: 99%

“…Despite an extensive geographical coverage of New Zealand, Lee's work was restricted to areas that were relatively easily accessible at that time. As a result, recent studies have unearthed a number of putative undescribed native species particularly in remote locations where no previous searches had been conducted (Boyer et al 2011;Buckley et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification and distribution of native and exotic earthworms in New Zealand human-modified soils

Kim¹,

Dickinson²,

Bowie³

et al. 2017

NZ J Ecol

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Important knowledge gaps remain with regards to the ecology and the systematics of New Zealand's native earthworms. With many putative new species yet to be described, often specimens cannot be named, which makes species inventory, monitoring and community comparisons difficult. Our work aimed to identify new putative taxa of New Zealand native species of earthworms, and describe their distribution in selected human-modified ecosystems. A total of 24 earthworm taxa (13 native and 11 exotic) were identified using a DNA barcoding approach focusing on 16S rDNA and COI (cytochrome oxidase subunit 1). The combination of morphological and molecular analyses were complementary in elucidating species identity. However, of the 13 native taxa, eight could not be named and are likely to be undescribed species from the genera Octochaetus, Maoridrilus and Deinodrilus. Most native species appeared to have a restricted geographic distribution linked to soil conditions, in particular pH and organic matter.

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Section: Specimen Identificationsupporting

confidence: 92%

Section: Delineation Of Molecular Taxonomic Units (Motus)mentioning

confidence: 99%

Section: Specimen Identificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular identification and distribution of native and exotic earthworms in New Zealand human-modified soils

Kim¹,

Dickinson²,

Bowie³

et al. 2017

NZ J Ecol

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“…Although this view was not generally supported (e.g. Blakemore 2005Blakemore , 2013) the accumulating molecular data suggest that the classical Octochaetidae is highly polyphyletic and the meroic states of the nephridial system were acquired independently in the different lineages (Buckley et al 2011, James andDavidson 2012).…”

Section: Disscussionmentioning

confidence: 99%

The second species of the endemic Malagasy earthworm genus Howascolex Michaelsen, 1901; Howascolex farafangana sp. n. (Clitellata, Megadrili)

Csuzdi¹,

Razafindrakoto²,

Hong³

2016

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A recent survey of earthworms carried out in South East Madagascar resulted in collecting two species belonging to the endemic Malagasy earthworm genus Howascolex Michaelsen, 1901. One of the species proved to be identical to the generotype Howascolex madagascariensis Michaelsen, 1901. The other species Howascolex farafangana sp. n. represents the second species of the genus. The genus Howascolex was originally classified in the subfamily Octochaetinae Michaelsen, 1900. However, its close morphological similarity with the Malagasy acanthodriline species implies a homoplasious origin of the meroic excretory system of Howascolex and its inclusion in the family Acanthodrilidae Claus, 1880.

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Estimating the biodiversity of terrestrial invertebrates on a forested island using DNA barcodes and metabarcoding data

Dopheide

Tooman

Grosser

et al. 2019

Ecological Applications

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Invertebrates are a major component of terrestrial ecosystems, however, estimating their biodiversity is challenging. We compiled an inventory of invertebrate biodiversity along an elevation gradient on the temperate forested island of Hauturu, New Zealand, by DNA barcoding of specimens obtained from leaf litter samples and pitfall traps. We compared the barcodes and biodiversity estimates from this data set with those from a parallel DNA metabarcoding analysis of soil from the same locations, and with pre‐existing sequences in reference databases, before exploring the use of combined data sets as a basis for estimating total invertebrate biodiversity. We obtained 1,282 28S and 1,610 COI barcodes from a total of 1,947 invertebrate specimens, which were clustered into 247 (28S) and 366 (COI) OTUs, of which ≤ 10% were represented in GenBank. Coleoptera were most abundant (730 sequenced specimens), followed by Hymenoptera, Diptera, Lepidoptera, and Amphipoda. The most abundant OTU from both the 28S (153 sequences) and COI (140 sequences) data sets was an undescribed beetle from the family Salpingidae. Based on the occurrences of COI OTUs along the elevation gradient, we estimated there are ~1,000 arthropod species (excluding mites) on Hauturu, including 770 insects, of which 344 are beetles. A DNA metabarcoding analysis of soil DNA from the same sites resulted in the identification of similar numbers of OTUs in most invertebrate groups compared with the DNA barcoding, but less than 10% of the DNA barcoding COI OTUs were also detected by the metabarcoding analysis of soil DNA. A mark–recapture analysis based on the overlap between these data sets estimated the presence of approximately 6,800 arthropod species (excluding mites) on the island, including ~3,900 insects. Estimates of New Zealand‐wide biodiversity for selected arthropod groups based on matching of the COI DNA barcodes with pre‐existing reference sequences suggested over 13,200 insect species are present, including 4,000 Coleoptera, 2,200 Diptera, and 2,700 Hymenoptera species, and 1,000 arachnid species (excluding mites). These results confirm that metabarcoding analyses of soil DNA tends to recover different components of terrestrial invertebrate biodiversity compared to traditional invertebrate sampling, but the combined methods provide a novel basis for estimating invertebrate biodiversity.

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Phylogenetic analysis of New Zealand earthworms (Oligochaeta: Megascolecidae) reveals ancient clades and cryptic taxonomic diversity

Cited by 72 publications

References 77 publications

Molecular identification and distribution of native and exotic earthworms in New Zealand human-modified soils

Molecular identification and distribution of native and exotic earthworms in New Zealand human-modified soils

The second species of the endemic Malagasy earthworm genus Howascolex Michaelsen, 1901; Howascolex farafangana sp. n. (Clitellata, Megadrili)

Estimating the biodiversity of terrestrial invertebrates on a forested island using DNA barcodes and metabarcoding data

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