Phylogenetic classification and clinical aspects of a new putative Deltapapillomavirus associated with skin lesions in cattle

Abstract

ABSTRACT. Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle. Thirteen types of BPVs have already been described and classified into 3 distinct genera. Divergences in the nucleotide sequence of the L1 gene are used to identify new viral types through the employment of PCR assays with degenerated primers. In the present study, a method for identifying BPVs based on PCR-RFLP and DNA sequencing allowed the identification of a new putative Deltapapillomavirus, designated JN/3SP (JQ280500.1). The analysis of the L1 gene showed that this Clinical aspects of a new putative BPV strain was most closely related to the BPVs -1, -2, -13 , and OaPV1 (71-73% genetic similarity). In this study, we describe the detection of this new putative Deltapapillomavirus type and verify its phylogenetic position within the genus.

Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects

“…We have studied the herd from which samples were obtained for the present study for the last 3 years, and have observed similar results to those reported in the literature (Araldi et al, 2013;Melo et al, 2014). A high incidence of cutaneous papillomatosis was observed in the herd studied, mainly in younger bovines of 2-3 years of age.…”

Molecular identification of bovine papillomaviruses in dairy and beef cattle: first description of Xi- and Epsilonpapillomavirus in Turkey

“…In the epidermis, mild to severe acanthosis, hyperkeratosis, prominent parakeratosis, mild to moderate basophilic cytoplasmic swelling, vacuolar degeneration of keratinocytes, and many koilocytes (including keratohyalin granules) were present. Moreover, similar histopathologic features in BPV infection have been previously reported in cattle from Brazil (15,23,24) and from Italy (17,25), and in water buffaloes and cattle from Turkey (19)(20)(21) and from India (26).…”

“…Although the identification of BPV types and epidemiological and clinical studies have provided new insights for the prevention and therapeutic procedures of papillomavirus infections (15), little is known about the presence of BPV in Turkey, except Deltapapillomavirus (BPV-1 and BPV-2) (19)(20)(21). The objective of the present study was to screen for the presence of different BPV types in cattle with papillomatosis as well as in healthyappearing cattle.…”

“…Polymerase chain reaction (PCR) remains an important tool for diagnostic purposes, particularly in determining asymptomatic carriers within the population, in clinical research, and in field investigations about viral infections (14). The L1 gene of papillomavirus has been used to detect BPVs by PCR using the degenerated FAP59/64 primer pair, which is the most conserved region (11,15). Nevertheless, the use of degenerated FAP59/64 primers together with type-specific primers is recommended (16,17), since the degenerated FAP59/64 primers have a low sensitivity for detecting new papillomavirus types and are unsuccessful for detecting some BPV types such as BPV-4 and BPV-9 (17).…”

Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection

“…The amplicons obtained were sequenced and virus typing showed the presence of BPV-2 in sample 1 (99% identity), BPV-11 in sample 2 (99% identity) and a new putative virus type in sample 3 (Figure 1). This new putative virus type was identified as BPV-JN/3SP (GenBank JQ280500.1) and was already described by Melo et al (2014). However, it was not possible to detect BPV with the FAP59/FAP64 primers in blood samples.…”