Phylogenetic Relationships and Intraspecific Variation of D-Genome Aegilops L. as Revealed by RAPD Analysis

Горюнова, С. В.; Кочиева, Е. З.; Chikida, N. N.; Pukhal’skii, V. A.

doi:10.1023/b:ruge.0000029154.79168.6f

Cited by 27 publications

(15 citation statements)

References 10 publications

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“…Ae. speltoides (S) has group 6 sequences but none of group 5, in correspondence with it being the most divergent of the species of section Sitopsis [46-53]. Note that Eig [37] put Ae.…”

Section: Discussionmentioning

confidence: 99%

Expansion of the gamma-gliadin gene family in Aegilops and Triticum

et al. 2012

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BackgroundThe gamma-gliadins are considered to be the oldest of the gliadin family of storage proteins in Aegilops/Triticum. However, the expansion of this multigene family has not been studied in an evolutionary perspective.ResultsWe have cloned 59 gamma-gliadin genes from Aegilops and Triticum species (Aegilops caudata L., Aegilops comosa Sm. in Sibth. & Sm., Aegilops mutica Boiss., Aegilops speltoides Tausch, Aegilops tauschii Coss., Aegilops umbellulata Zhuk., Aegilops uniaristata Vis., and Triticum monococcum L.) representing eight different genomes: Am, B/S, C, D, M, N, T and U. Overall, 15% of the sequences contained internal stop codons resulting in pseudogenes, but this percentage was variable among genomes, up to over 50% in Ae. umbellulata. The most common length of the deduced protein, including the signal peptide, was 302 amino acids, but the length varied from 215 to 362 amino acids, both obtained from Ae. speltoides. Most genes encoded proteins with eight cysteines. However, all Aegilops species had genes that encoded a gamma-gliadin protein of 302 amino acids with an additional cysteine. These conserved nine-cysteine gamma-gliadins may perform a specific function, possibly as chain terminators in gluten network formation in protein bodies during endosperm development. A phylogenetic analysis of gamma-gliadins derived from Aegilops and Triticum species and the related genera Lophopyrum, Crithopsis, and Dasypyrum showed six groups of genes. Most Aegilops species contained gamma-gliadin genes from several of these groups, which also included sequences from the genera Lophopyrum, Crithopsis, and Dasypyrum. Hordein and secalin sequences formed separate groups.ConclusionsWe present a model for the evolution of the gamma-gliadins from which we deduce that the most recent common ancestor (MRCA) of Aegilops/Triticum-Dasypyrum-Lophopyrum-Crithopsis already had four groups of gamma-gliadin sequences, presumably the result of two rounds of duplication of the locus.

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“…Ae. speltoides (S) has group 6 sequences but none of group 5, in correspondence with it being the most divergent of the species of section Sitopsis [46-53]. Note that Eig [37] put Ae.…”

Section: Discussionmentioning

confidence: 99%

Expansion of the gamma-gliadin gene family in Aegilops and Triticum

et al. 2012

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“…cylindrica is known as a source of useful traits such as salinity tolerance (Farooq et al 1992), Hessian fly resistance (El Bouhssini et al 1998) and cold tolerance (Iriki et al 2001). Genetic diversity in this species was evaluated using allozymic diversity (Watanabe et al 1994), RAPD markers (Okuno et al 1998;Goryunova et al 2004), a combination of RAPD and AFLP markers (Pester et al 2003) and polymorphism of DNA sequences (Caldwell et al 2004). The distribution of Ae.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the D-genome in T. aestivum and Aegilops species using SSR markers

Naghavi

Aghaei

Taleei

et al. 2008

Genet Resour Crop Evol

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Simple sequence repeats (SSRs), highly dispersed nucleotide sequences in genomes, were used for germplasm analysis and estimation of the genetic relationship of the D-genome among 52 accessions of T. aestivum (AABBDD), Ae. tauschii (D t D t ), Ae. cylindrica (CCD c D c ) and Ae. crassa (MMD cr1 D cr1 ), collected from 13 different sites in Iran. A set of 21 microsatellite primers, from various locations on the seven D-genome chromosomes, revealed a high level of polymorphism. A total of 273 alleles were detected across all four species and the number of alleles per each microsatellite marker varied from 3 to 27. The highest genetic diversity occurred in Ae. tauschii followed by Ae. crassa, and the genetic distance was the smallest between Ae. tauschii and Ae. cylindrica. Data obtained in this study supports the view that genetic variability in the D-genome of hexaploid wheat is less than in Ae. tauschii. The highest number of unique alleles was observed within Ae. crassa accessions, indicating this species as a great potential source of novel genes for bread wheat improvement. Knowledge of genetic diversity in Aegilops species provides different levels of information which is important in the management of germplasm resources.

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“…recta, and that A. ventricosa presumably derives from natural hybridization between A. tauschii L. and A. uniaristata (Tsunewaki et al 1991). Further credence for this hypothesis was based on cytogenetic studies, on observations of an improved meiotic pairing in hybrids (Zhang et al 1992) and on phylogenetic relationships revealed by RAPD analysis and by single copy nuclear genes (Goriunova et al 2004;Petersen et al 2006). …”

Section: Discussionmentioning

confidence: 95%

Diversity analysis of Aegilops species from Morocco using RAPD markers

Belkadi

Assali

Filali-Maltouf

et al. 2010

Genet Resour Crop Evol

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The diversity of 51 representative populations of the 5 Aegilops species from Moroccan collection was analyzed using 22 RAPD primers. We investigated the associations among these 5 Aegilops species (A. geniculata Roth (UUMM), A. triuncialis L. (UUCC), A. ventricosa Tausch (DDNN), A. peregrina (Hackel) Maire et Weiller (UUMM) and A. neglecta Req. ex Bert. subsp. recta (Zhuk.) K.Hammer (UUMMNN)); some diploid species considered as their ancestors; accessions of some neighboring countries and also accessions of Triticums. A total of 650 polymorphic RAPD fragments were amplified. A dendrogram was constructed using the un-weighed pair group method arithmetic average (UPGMA) and Jaccard's similarity coefficient. The UPGMA clustering showed a regrouping to the level species with high level of the structuration of the diversity at A. geniculata. We confirm as reported by other authors, the proximity of N genome to U genome and C genome to M genome and also the difference between the genomes M and N. Thus, the phylogeny between the species and the different genomes were retracted.

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Phylogenetic Relationships and Intraspecific Variation of D-Genome Aegilops L. as Revealed by RAPD Analysis

Cited by 27 publications

References 10 publications

Expansion of the gamma-gliadin gene family in Aegilops and Triticum

Expansion of the gamma-gliadin gene family in Aegilops and Triticum

Genetic diversity of the D-genome in T. aestivum and Aegilops species using SSR markers

Diversity analysis of Aegilops species from Morocco using RAPD markers

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