Phylogenomic Analysis Supports Two Possible Origins for Latin American Strains of Vibrio parahaemolyticus Associated with Acute Hepatopancreatic Necrosis Disease (AHPND)

González-Gómez, Jean Pierre; Soto-Rodríguez, Sonia A.; López-Cuevas, Osvaldo; Campo, Nohelia Castro‐del; Cháidez, Cristóbal; Gómez‐Gil, Bruno

doi:10.1007/s00284-020-02214-w

Cited by 15 publications

(11 citation statements)

References 48 publications

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“…In addition, some deletion in the PirAB region were also found in two of the Latin American strains. Interestingly, the study demonstrates that diagnosis of AHPND through PirAB toxin gene detection may be inadequate due to structural variability of these genes, as noticed in different isolates [ 86 ].…”

Section: Acute Hepatopancreatic Necrosis Disease (Ahpnd)—an Overviewmentioning

confidence: 99%

Acute Hepatopancreatic Necrosis Disease (AHPND): Virulence, Pathogenesis and Mitigation Strategies in Shrimp Aquaculture

Kumar

Roy

Behera

et al. 2021

Toxins

126

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Shrimp, as a high-protein animal food commodity, are one of the fastest growing food producing sectors in the world. It has emerged as a highly traded seafood product, currently exceeding 8 MT of high value. However, disease outbreaks, which are considered as the primary cause of production loss in shrimp farming, have moved to the forefront in recent years and brought socio-economic and environmental unsustainability to the shrimp aquaculture industry. Acute hepatopancreatic necrosis disease (AHPND), caused by Vibrio spp., is a relatively new farmed penaeid shrimp bacterial disease. The shrimp production in AHPND affected regions has dropped to ~60%, and the disease has caused a global loss of USD 43 billion to the shrimp farming industry. The conventional approaches, such as antibiotics and disinfectants, often applied for the mitigation or cure of AHPND, have had limited success. Additionally, their usage has been associated with alteration of host gut microbiota and immunity and development of antibiotic resistance in bacterial pathogens. For example, the Mexico AHPND-causing V. parahaemolyticus strain (13-306D/4 and 13-511/A1) were reported to carry tetB gene coding for tetracycline resistance gene, and V. campbellii from China was found to carry multiple antibiotic resistance genes. As a consequence, there is an urgent need to thoroughly understand the virulence mechanism of AHPND-causing Vibrio spp. and develop novel management strategies to control AHPND in shrimp aquaculture, that will be crucially important to ensure food security in the future and offer economic stability to farmers. In this review, the most important findings of AHPND are highlighted, discussed and put in perspective, and some directions for future research are presented.

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Section: Acute Hepatopancreatic Necrosis Disease (Ahpnd)—an Overviewmentioning

confidence: 99%

Acute Hepatopancreatic Necrosis Disease (AHPND): Virulence, Pathogenesis and Mitigation Strategies in Shrimp Aquaculture

Kumar

Roy

Behera

et al. 2021

Toxins

126

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“…Hence, confirmatory diagnosis of AHPND in shrimp should include, in addition to clinical signs, i.e., the histopathological lesions observed in the acute stage of the disease, molecular detection of pirA Vp and pirB Vp genes coupled with bioassays. Diagnosis of AHPND through only pirA Vp and pirB Vp genes detection may be inadequate due to instability of these genes, as observed in strains isolated from different geographical regions [25].…”

Section: Acute Hepatopancreatic Necrosis Disease In Penaeid Shrimpmentioning

confidence: 99%

“…AHPND is mainly caused by Vp that harbors a plasmid of ~70 kbp pVA1 containing the pirAVp and pirBVp genes, which encode a delta-endotoxin responsible for the typical lesions in shrimp Hp [1,10). The genomes of pVA1-harboring Vp revealed a large pan genome, high genetic diversity grouped into three main clades, and specific structural differences, in addition to the instability of the pirAB Vp region of the pVA1 plasmid [25]. The structural differences found in pVA1 are likely due to horizontal propagation of the plasmid to other Vibrio species [19], such as V. harveyi [4,18], V. campbellii and V. owensii [18], and V. punensis [17. The above processes might cause the appearance of new pathogenic AHPND strains and a major threat for the shrimp industry.…”

Section: Virulence Plasmid Pva1mentioning

confidence: 99%

PirAB from <em>Vibrio parahaemolyticus</em> Exhibits a Binary Toxin Action with Bactericidal Activity and Cytotoxicity in <em>Penaeus vannamei</em>

Soto-Rodríguez¹,

Lozano‐Olvera²,

Montfort³

et al. 2022

Preprint

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PirAB is a binary protein complex secreted by specific strains of Vibrio parahaemolyticus (Vp) that harbor the pVA1 virulence plasmid and express PirAVp and PirBVp toxins. PirABVp causes acute hepatopancreatic necrosis disease (AHPND), a newly emergent penaeid shrimp disease that can cause 70–100% mortality and has resulted in great economic losses since its appearance. The cy-totoxic effect of PirABVp on the epithelial cell of the shrimp hepatopancreas has been extensively reported. Our studies found that the PirBVp subunit has lectin activity and recognizes mucin-like O-glycosidic structures in the shrimp hepatopancreas. The PirAVp subunit may have a stabiliza-tion function of the binary complex. However, we also found that Vp AHPND changes the water microbiota community structure and causes a significant reduction in several bacteria, especially Neptuniibacter spp. We propose that the PirABvp toxin could exhibit a dual role: damage the shrimp hepatopancreas and kill surrounding bacteria.

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“…Así como las cepas de Sinaloa y Thailandia presentan elevada diversidad de polimorfismo en las cepas patógenas de V. parahaemolyticus, algunas con la máxima virulencia entre las 7-10 h con mortalidades del 50% y las menos virulentas con mortalidades a las 17 h en los retos experimentales (Joshi et al, 2014;Soto-Rodriguez et al, 2015). En nuestros resultados se diferencian de las cepas de Tailandia, China y el Noreste de México (Sinaloa y Sonora) con base en las secuencias de la región 16S ADNr, lo cual concuerda con reportes recientes que reconocen tres cladas filogenéticas basado en la alineación de secuencias nucleotídicas del plásmido pVA1 que hospedan (Latinoamericana, Asiática y Cosmopolita), diferenciándose en la ausencia del transposón Tn3 en la clada Asiática y además, algunas deleciones (pérdida de secuencias de ADN o nucleótidos) en la región pirAB encontrada en las cepas Latinoamericanas (González-Gómez et al, 2020).…”

Section: Discusionunclassified

Vibrio parahaemolyticus strains causing acute hepatopancreatic necrosis disease in farming shrimp of Sonora, Mexico and their antibiotic resistance

Galaviz‐Silva¹

2021

Hidrobiológica

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Phylogenomic Analysis Supports Two Possible Origins for Latin American Strains of Vibrio parahaemolyticus Associated with Acute Hepatopancreatic Necrosis Disease (AHPND)

Cited by 15 publications

References 48 publications

Acute Hepatopancreatic Necrosis Disease (AHPND): Virulence, Pathogenesis and Mitigation Strategies in Shrimp Aquaculture

Acute Hepatopancreatic Necrosis Disease (AHPND): Virulence, Pathogenesis and Mitigation Strategies in Shrimp Aquaculture

PirAB from <em>Vibrio parahaemolyticus</em> Exhibits a Binary Toxin Action with Bactericidal Activity and Cytotoxicity in <em>Penaeus vannamei</em>

Vibrio parahaemolyticus strains causing acute hepatopancreatic necrosis disease in farming shrimp of Sonora, Mexico and their antibiotic resistance

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