Phylogeny of Zika Virus in Western Hemisphere, 2015

Lanciotti, Robert S.; Lambert, Amy J.; Holodniy, Mark; Saavedra, Sonia; Signor, Leticia del Carmen Castillo

doi:10.3201/eid2205.160065

Cited by 323 publications

(304 citation statements)

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“…ZIKV clusters phylogenetically into African and Asian genotypes, and the virus implicated in the American outbreak of ZIKV clusters within the Asian lineage (9). Recent reports have shown that Aedes aegypti mosquitoes are highly competent vectors for American isolates of ZIKV (10).…”

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confidence: 99%

“…Therefore, we developed and tested an infectious clone of a ZIKV strain (PRVABC59) recently isolated by the CDC from a human infected in Puerto Rico in 2015 (9) and demonstrated its growth characteristics in cell culture, A. aegypti mosquitoes, and a mouse model of pathogenesis. Virus rescued from this clone is similar in RNA sequence, in vitro replication, mouse pathogenesis, and mosquito transmission to the parental ZIKV isolate.…”

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confidence: 99%

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Development and Characterization of Recombinant Virus Generated from a New World Zika Virus Infectious Clone

Weger-Lucarelli

Duggal

Bullard-Feibelman

et al. 2017

J Virol

101

112

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Zika virus (ZIKV; family Flaviviridae, genus Flavivirus) is a rapidly expanding global pathogen that has been associated with severe clinical manifestations, including devastating neurological disease in infants. There are currently no molecular clones of a New World ZIKV available that lack significant attenuation, hindering progress toward understanding determinants of transmission and pathogenesis. Here we report the development and characterization of a novel ZIKV reverse genetics system based on a 2015 isolate from Puerto Rico (PRVABC59). We generated a two-plasmid infectious clone system from which infectious virus was rescued that replicates in human and mosquito cells with growth kinetics representative of wildtype ZIKV. Infectious clone-derived virus initiated infection and transmission rates in Aedes aegypti mosquitoes comparable to those of the primary isolate and displayed similar pathogenesis in AG129 mice. This infectious clone system provides a valuable resource to the research community to explore ZIKV molecular biology, vaccine development, antiviral development, diagnostics, vector competence, and disease pathogenesis.IMPORTANCE ZIKV is a rapidly spreading mosquito-borne pathogen that has been linked to Guillain-Barré syndrome in adults and congenital microcephaly in developing fetuses and infants. ZIKV can also be sexually transmitted. The viral molecular determinants of any of these phenotypes are not well understood. There is no reverse genetics system available for the current epidemic virus that will allow researchers to study ZIKV immunity, develop novel vaccines, or develop antiviral drugs. Here we provide a novel infectious clone system generated from a recent ZIKV isolated from a patient infected in Puerto Rico. This infectious clone produces virus with in vitro and in vivo characteristics similar to those of the primary isolate, providing a critical tool to study ZIKV infection and disease.

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confidence: 99%

mentioning

confidence: 99%

Development and Characterization of Recombinant Virus Generated from a New World Zika Virus Infectious Clone

Weger-Lucarelli

Duggal

Bullard-Feibelman

et al. 2017

J Virol

101

112

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“…Amerika kıtasından elde edilen suşların değerlendirildiği bir çalışmada ise bu suşlarla Fransız Polinezyası'ndan elde edilen suşlar arasında % 99'un üzerinde nükleotid benzerliği saptanmıştır. Bu suşlar Asya genotipi içinde "Batı Yarımküre grubu"nu oluştur-maktadır (47) .…”

Section: Vi̇roloji̇unclassified

Novel Global Threat: Zika Virus Infections

Nemli¹,

Demırdal²

2016

Ankem Derg

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“…It is a positive-sense, single-stranded RNA virus of approximately 11 kb in genome length. ZIKV is a member of the Spondweni serocomplex and has evolved into three separate genotypes (West African, East African and Asian) based on the phylogenetic relationships (Haddow et al, 2012;Lanciotti et al, 2016). Since the first discovery of ZIKV in Uganda in 1947, only 14 human cases of Zika virus disease have been reported and all were restricted to Africa and Southeast Asia, such as Uganda, Nigeria, Senegal, and Indonesia (Moore et al, 1975;Olson and Ksiazek, 1981;Duffy et al, 2009;Enserink, 2015 (Duffy et al, 2009).…”

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confidence: 99%

“…Furthermore, ZIKV have been prevailing in Southeast Asian for a long time and may be experiencing a geographic expansion event within Asian lineage (Haddow et al, 2012;Lanciotti et al, 2016). It is likely that ZIKV infection cases could be greatly underestimated or misdiagnosed in Asia, due to the symptoms clinically similar to dengue fever and many other tropical infectious diseases (Haddow et al, 2012).…”

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confidence: 99%