Phylogeography and classification of Dusty Miller (Spyridium parvifolium; Rhamnaceae): a morphologically variable shrub from south-east Australia

Abstract

Spyridium parvifolium is a widespread and morphologically variable shrub from south-eastern Australia. Several varieties have been recognised, and there is disagreement on the accepted taxonomy between Australian states. This study investigated the phylogeography of the species and assessed genetic distinctiveness of its morphological variants. Nuclear ribosomal DNA and complete chloroplast genomes from seventy-two samples of S. parvifolium and seven samples from closely related species were sequenced and analysed using both Bayesian and maximum likelihood phylogenetic methods. The results showed incongruence in the placement of several associated taxa (S. cinereum, S. obcordatum and S. daltonii), plausibly due to long branch attraction, introgression or incomplete lineage sorting. Spyridium parvifolium was resolved as paraphyletic in both phylogenies, with accessions from west of the Murray Darling Depression divergent from those east of the Depression. We found evidence of isolation within S. parvifolium on the inland side of the Great Dividing Range and recent gene flow across Bass Strait. The variants of S. parvifolium were not supported as genetically distinct, and with the prevalence of several variants at single sites and morphological intergrades between variants, we conclude that the taxon is a single, morphologically variable species and that no infraspecific classification is warranted.

Phylogenetic Analysis of Rare and Endangered Tulipa Species (Liliaceae) of Kazakhstan Based on Universal Barcoding Markers

“…For nuclear DNA alignment, Markov chain Monte Carlo analyses were conducted with four chains for 10 M generations, sampling every 1000 steps, with a burnin of 25% and an unlinked Dirichlet distribution. For the chloroplast DNA alignment, the analysis was run for 2.5 M generations, sampling every 500 steps [ 59 ]. The output files were viewed in Tracer to check for convergence [ 60 ].…”