Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice

Abstract

A chronic high-fat diet (HFD) produces obesity, leading to pathological consequences in the liver and skeletal muscle. The fat in the liver leads to accumulation of a large number of intrahepatic lipid droplets (LD), which are susceptible to oxidation. Obesity also affects skeletal muscle, increasing LD and producing insulin signaling impairment. Physalis peruviana L. (PP) (Solanaceae) is rich in peruvioses and has high antioxidant activity. We assessed the ability of PP to enhance insulin-dependent glucose uptake in skeletal muscle and the capacity to prevent both inflammation and lipoperoxidation in the liver of diet-induced obese mice. Male C57BL/6J mice were divided into groups and fed for eight weeks: control diet (C; 10% fat, 20% protein, 70% carbohydrates); C + PP (300 mg/kg/day); HFD (60% fat, 20% protein, 20% carbohydrates); and HFD + PP. Results suggest that PP reduces the intracellular lipoperoxidation level and the size of LD in both isolated hepatocytes and skeletal muscle fibers. PP also promotes insulin-dependent skeletal muscle glucose uptake. In conclusion, daily consumption of 300 mg/kg of fresh pulp of PP could be a novel strategy to prevent the hepatic lipoperoxidation and insulin resistance induced by obesity.

Plasma Metabolome Profiling by High-Performance Chemical Isotope-Labelling LC-MS after Acute and Medium-Term Intervention with Golden Berry Fruit (Physalis peruviana L.), Confirming Its Impact on Insulin-Associated Signaling Pathways

“…Our findings and mechanistic hypothesis based on our metabolomic approach are supported by a recent report from Pino-de-la Fuente et al Indeed, the authors recorded positive effects of Physalis peruviana in vivo, i.e., insulin resistance and inflammation improvement in the muscles and liver in a mouse model of diet-induced obesity. These data point in the same direction as ours at the molecular and physiological levels in relation to the compositions of golden berry fruit, as suggested by the authors [52].…”

EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet

“…The effect of these products on different metabolic parameters classically affected in obesity was also analyzed. The HFD model induced an obese phenotype as has been reported before [34,35]. Physiological measurements show that the different fish oil products have a discrete impact on mouse physiology.…”

“…Our results support that the administration of EPA and DHA concentrate from fish oil using RSM is capable of reducing the obesity-associated hyperinsulinemia. Although the three kinds of EPA/DHA supplementation did not improve the lipid profile, neither lipidic hepatocyte content nor oxidized LD content are classically altered in HFD-fed mice [11,34]. The statistical result of Figure 4b shows that the highest oxidized group was B, while groups C and D showed a lower level of oxidized lipids in hepatocytes.…”

“…Hepatocytes were purified by density gradient centrifugation in Ficoll (Sigma, St. Louis, MO, USA); after centrifugation at 2660× g for 90 s the hepatocyte pellet was washed 3 times with PBS. This preparation was used to perform immunofluorescence and lipoperoxidation labeling analysis with 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid (BODIPY ® 581/591 C11) (Invitrogen, Carlsbad, CA, USA) as reported previously [34].…”

Chemical constituents and evidence-based pharmacological properties of Physalis peruviana L.: An overview

“…Recently, in obese mice, induced by a regular diet rich in fats, oral and daily administration of pulp extract of (the dose of 300 mg/ kg bw) improved insulin resistance in skeletal muscles by reducing both serum insulin and blood sugar levels. Also, the plant extract protected the liver against oxidative stress and improved the inflammatory state (81). The hydroalcoholic extract of the leaves of P. peruviana and its fractions (hexane, ethyl acetate, and ethyl acetate residue) showed antidiabetic activity in streptozotocin-induced diabetic rats after 28 days of treatment.…”