Physical and chemical characterization insulin-loaded chitosan-TPP nanoparticles

Azevedo, J. R.; Sizilio, R. H.; Brito, M. B.; Costa, Andréa Machado; Serafini, Mairim Russo; Araújo, A. A. S.; Santos, Márcio Roberto Viana dos; Lira, Ana Amélia Moreira; Nunes, Rogéria de Souza

doi:10.1007/s10973-011-1429-5

Cited by 68 publications

(32 citation statements)

References 25 publications

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“…1). This technique has been widely used to prepare CNs as drug delivery systems for a variety of drugs, including either hydrophobic drugs or hydrophilic protein drugs [22,24]. Because of the strong hydrophobicity of MIF, it is hard to load MIF into blank CNs after the ionic gelation process.…”

Section: Resultsmentioning

confidence: 99%

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

Zhang¹,

Wu²,

Li³

et al. 2016

Beilstein J. Nanotechnol.

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In addition to its well-known abortifacient effect, mifepristone (MIF) has been used as an anticancer drug for various cancers in many studies with an in-depth understanding of the mechanism of action. However, application of MIF is limited by its poor water solubility and low oral bioavailability. In this work, we developed a drug delivery system based on chitosan nanoparticles (CNs) to improve its bioavailability and anticancer activity. The MIF-loaded chitosan nanoparticles (MCNs) were prepared by convenient ionic gelation techniques between chitosan (Cs) and tripolyphosphate (TPP). The preparation conditions, including Cs concentration, TPP concentration, Cs/MIF mass ratio, and pH value of the TPP solution, were optimized to gain better encapsulation efficiency (EE) and drug loading capacity (DL). MCNs prepared with the optimum conditions resulted in spherical particles with an average size of 200 nm. FTIR and XRD spectra verified that MIF was successfully encapsulated in CNs. The EE and DL of MCNs determined by HPLC were 86.6% and 43.3%, respectively. The in vitro release kinetics demonstrated that MIF was released from CNs in a sustained-release manner. Compared with free MIF, MCNs demonstrated increased anticancer activity in several cancer cell lines. Pharmacokinetic studies in male rats that were orally administered MCNs showed a 3.2-fold increase in the area under the curve from 0 to 24 h compared with free MIF. These results demonstrated that MCNs could be developed as a potential delivery system for MIF to improve its anticancer activity and bioavailability.

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Section: Resultsmentioning

confidence: 99%

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

Zhang¹,

Wu²,

Li³

et al. 2016

Beilstein J. Nanotechnol.

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“…The imine -C= N groups (possible formed during GA crosslinking of chitosan or as result of -CHO reaction of periodate oxidized cotton with chitosan) have characteristic bands around 1640 cm −1 [35]-1680 cm −1 [10]; they are difficult to be evidenced due their possible overlapping with the amide characteristic band (1650 cm −1 ). Spectrum 4 (sample VT-3) presents additional bands around 1100-1200 cm −1 , which can be attributed to P = O stretching [36,18]; the broadening of the band at 1554 cm −1 can be attributed to the electrostatic interactions between amine groups of the chitosan and carboxylic groups of the support [37,38]. The band at 1161 cm −1 can be attributed to the interactions between amine chitosan and phosphate groups, as reported for the chitosan-chondroitinsulfate interpolymeric complex [39].…”

Section: Resultsmentioning

confidence: 99%

Bioactive cotton fabrics containing chitosan and biologically active substances extracted from plants

Mocanu¹,

Nichifor²,

Mihai³

et al. 2013

Materials Science and Engineering: C

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“…The system was purged with nitrogen gas at the rate of 100 ml/min to maintain inert atmosphere. All samples were run in triplicate 4 .…”

Section: Methods: Drug-polymer Compatibility Study By Dsc Thermal Anamentioning

confidence: 99%

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2018

IJPSR

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The overall objective of this research is to improve the oral bioavailability of insulin through encapsulation in nanoparticles formulated by "ionotropic pre-gelation followed by polyelectrolyte complexation technique". The preparation variables such as initial drug concentration, polymer: polymer ratios, crosslinker concentration, stirring speed, stirring time, pH of drug / polymer mixture were investigated to study the effect of variables on nanoparticles size and drug entrapment efficiency. The optimum formula of insulin-loaded nanoparticles was tested for insulin release in three different pH media. The pharmacological activity of insulin-loaded nanoparticles was evaluated following oral dosage in diabetic rats and then study whether insulinloaded nanoparticles would induce hypoglycemic effect after oral administration to diabetic rats. The optimum formula of nanoparticles improved insulin release characteristics. Thus, the polymer matrix provided protection for insulin in acidic gastric medium and allowed prolonged insulin release in alkaline intestinal medium. In-vivo results indicated that nanoparticles kept insulin bioactivity and its hypoglycemic effect after oral administration of insulinloaded nanoparticles to diabetic rat model. It was found that natural biodegradable nanoparticles are a promising device for oral insulin delivery.

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Physical and chemical characterization insulin-loaded chitosan-TPP nanoparticles

Cited by 68 publications

References 25 publications

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

Bioactive cotton fabrics containing chitosan and biologically active substances extracted from plants

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