Physical exercise increases mitochondrial function and reduces oxidative damage in skeletal muscle

Silva, Luciano Acordi da; Pinho, Cleber A.; Scarabelot, Karoline Sousa; Fraga, Daiane B.; Volpato, Ana Maria; Boeck, Carina Rodrigues; Souza, Cláudio Teodoro de; Streck, Emílio L.; Pinho, Ricardo Aurino

doi:10.1007/s00421-008-0971-8

Cited by 59 publications

(62 citation statements)

References 49 publications

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“…Resultados similares são apontados por outros autores (21) , no que diz respeito a mudanças enzimáticas no funcionamento mitocondrial após o treinamento. Dados do nosso grupo mostram que a atividade da SDH e dos quatro complexos da CTE são elevados após oito semanas de treinamento em músculo esquelético (22) . Contudo, os resultados do presente estudo demonstram que o fígado responde de maneira similar.…”

Section: Discussionunclassified

Comparação do treinamento físico de quatro e oito semanas sobre atividade da cadeia transportadora de elétrons e marcadores de estresse oxidativo em fígado de camundongos

Silva

Ronsani

Souza

et al. 2010

Rev Bras Med Esporte

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RESUMOO presente estudo investigou o efeito de quatro e oito semanas de treinamento físico sobre a atividade dos complexos da cadeia transportadora de elétrons (CTE) e os marcadores de estresse oxidativo em fígado de camundongos. Vinte e um camundongos (CF1, 30-35g) foram distribuídos nos seguintes grupos: não treinado (NT); treinado quatro semanas (T4); treinado oito semanas (T8). Quarenta e oito horas após a última sessão de treinamento os animais foram mortos por decapitação e o fígado foi retirado e estocado em -70ºC para posterior análise. Atividade da succinato desidrogenase (SDH), dos complexos I,II,III e IV da CTE, carbonilação de proteína, conteúdo total de tióis e a atividade da superóxido dismutase foram mensurados. Os resultados demonstram que apenas oito semanas de treinamento aumentam a atividade da SDH, dos quatro complexos da CTE, da superóxido dismutase, e o conteúdo total de tióis em relação ao grupo não treinado. Houve ainda diminuição na carbonilação de proteína no respectivo grupo em relação ao NT. Em conclusão, são necessárias oito semanas de treinamento para que ocorram aumento no funcionamento mitocondrial e melhora nos marcadores de estresse oxidativo em fígado de camundongos. Palavras-chave: treinamento físico, cadeia transportadora de elétrons, estresse oxidativo ABSTRACTThe present study investigated mitochondrial adaptations and oxidative stress markers after four and eight weeks of running training in liver of mice. Twenty-one male mice (CF1, 30-35g) were distributed into the following groups (n=7): untrained (UT); trained -four weeks (T4); trained -eight weeks (T8). Forty-eight hours after the last training session the animals were killed by decapitation and livers were removed and stored at -70ºC. Succinate dehydrogenase (SDH), complexes I, II, II-III and IV, protein carbonyls (PC), total thiol content and superoxide dismutase activity were measured. The results show that endurance training (8-wk) increases the SDH activity and complexes (I, II, III, IV), superoxide dismutase and total thiol content in liver when compared to untrained animals. Decrease in protein carbonylation in the respective group in relation to UT was also observed. It could be concluded that eight weeks of running training are necessary for mitochondrial respiratory chain enzyme activities increase and improvement in oxidative stress markers in liver of mice.Keywords: physical training, electron transport chain, oxidative stress INTRODUÇÃOEstudos têm reportado que o exercício físico aumenta a produção das espécies reativas de oxigênio (ERO) e, consequentemente, estresse oxidativo em diversos órgãos e tecidos (1,2) . Tem sido sugerido que a elevação do consumo de oxigênio induzida pelos exercícios aumenta a atividade mitocondrial, gerando altas concentrações de ERO (3) . O estresse oxidativo (EO) altera a integridade de constituintes celulares e compromete todo o funcionamento celular (1) . Células hepáticas precisam de energia para realizar diversas funções. A alta taxa metabólica do fígado (200kcal/kg/tecido...

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Section: Discussionunclassified

Comparação do treinamento físico de quatro e oito semanas sobre atividade da cadeia transportadora de elétrons e marcadores de estresse oxidativo em fígado de camundongos

Silva

Ronsani

Souza

et al. 2010

Rev Bras Med Esporte

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“…Mechanisms responsible for beneficial effects of chronic aerobic exercise are traininginduced up-regulation of SOD [11] and CAT [12], reduced mitochondrial reactive oxygen species [13], and reduced expression of NADPH oxidase [14]. It has been proposed that chronic high-intensity interval training (HIIT) (≈85% VO 2max ) may elicit greater health benefits than traditional chronic aerobic exercise.…”

Section: Introductionmentioning

confidence: 99%

High intensity interval training favourably affects antioxidant and inflammation mRNA expression in early-stage chronic kidney disease

Tucker

Briskey

Scanlan

et al. 2015

Free Radical Biology and Medicine

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Increased levels of oxidative stress and inflammation have been linked to the progression of chronic kidney disease. To reduce oxidative stress and inflammation related to chronic kidney disease, chronic aerobic exercise is often recommended. Data suggests high intensity interval training may be more beneficial than traditional aerobic exercise. However, appraisals of differing modes of exercise, along with explanations of mechanisms responsible for observed effects, are lacking. This study assessed effects of eight weeks of high intensity interval training (85% VO 2 max), versus low intensity exercise (45-50% VO 2 max) and sedentary behaviour, in an animal model of early-stage chronic kidney disease. We examined kidney-specific mRNA expression of genes related to endogenous antioxidant enzyme activity (glutathione peroxidase 1; Gpx1, superoxide dismutase 1; Sod1, and catalase; Cat) and inflammation (kidney injury molecule 1; Kim1 and tumour necrosis factor receptor super family 1b; Tnfrsf1b), as well as plasma F2-isoprostanes, a marker of lipid peroxidation.Compared to sedentary behaviour, high intensity interval training resulted in increased mRNA expression of Sod1 (p=0.01) and Cat (p<0.001). Compared to low intensity exercise, high intensity interval training resulted in increased mRNA expression of Cat (p<0.001) and Tnfrsf1b (p=0.047). In this study, high intensity interval training was superior to sedentary behaviour and low intensity exercise as high intensity interval training beneficially influenced expression of genes related to endogenous antioxidant enzyme activity and inflammation.

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“…Excessive production of these species and consequent oxidative damages have been associated with decreased performance, fatigue, muscle damage, and "overtraining" 23,24 . Although strenuous exercise promotes increased production of ROS and oxidative damage 6,4 , studies have shown that endurance training increases the antioxidant defense system, as well as muscle oxidative capacity 24,25 . However, these positive effects of training may not be sufficient to reduce oxidative damage caused by intense exercise of long duration.…”

Section: Discussionmentioning

confidence: 99%

Impacto do triatlon ironman sobre os parametros de estresse oxidative. DOI:10.5007/1980-0037.2012v14n2p174

Scheffer¹,

Pinho²,

Hoff

et al. 2012

Rev. Bras. Cineantropom. Desempenho Hum.

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-Several studies have investigated the biochemical response changes that take place in Ironman triathletes, but there are few data on oxidative stress changes. The objective of this study was to investigate oxidative stress parameters in triathletes after an Ironman event. The sample consisted of eighteen male triathletes, with a mean age of 34.5 ± 2.15 years, weight 69.3 ± 1.9 kg, and height 1.71 ± 0.18 m. The Ironman triathlon consists of a 3.8-km swim, a 180-km bicycle ride, and a 42.2-km (marathon) run. Before the competition and immediately after its conclusion, 10-mL blood samples were collected, centrifuged and frozen at -80ºC for subsequent analysis. Total antioxidant capacity, lipid peroxidation, protein carbonylation, and total thiol content were measured. The results showed a significant increase in all markers after the event (p<0.05) in relation to the pre--event period, which conclusively shows that the Ironman triathlon induces significant changes oxidative stress markers in athletes and that antioxidant supplementation would be important to reverse these effects.

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Physical exercise increases mitochondrial function and reduces oxidative damage in skeletal muscle

Cited by 59 publications

References 49 publications

Comparação do treinamento físico de quatro e oito semanas sobre atividade da cadeia transportadora de elétrons e marcadores de estresse oxidativo em fígado de camundongos

Comparação do treinamento físico de quatro e oito semanas sobre atividade da cadeia transportadora de elétrons e marcadores de estresse oxidativo em fígado de camundongos

High intensity interval training favourably affects antioxidant and inflammation mRNA expression in early-stage chronic kidney disease

Impacto do triatlon ironman sobre os parametros de estresse oxidative. DOI:10.5007/1980-0037.2012v14n2p174

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