Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K

Rahman, Raja Noor Zaliha Abd.; Geok, Lee Poh; Basri, Mahiran; Salleh, Abu Bakar

doi:10.1016/j.biortech.2004.06.012

Cited by 132 publications

(81 citation statements)

References 20 publications

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“…Maximum production of extra cellular protease after 120 hours of incubation by Streptomyces sp. 594, which was not growth associated, was reported by Azeredo et al [25]. Our results were in concurrence with them.…”

Section: Discussionsupporting

confidence: 93%

“…S. albolongus preferred medium with neutral pH for maximum enzyme production, but maximum protease activities S. aburaviensis was obtained in medium having initial p H 8.0. Production of microbial proteases in neutral and alkaline medium p H was also reported by other authors [25][26][27]. Temperature is an important environmental factor for growth and enzyme production.…”

Section: Discussionsupporting

confidence: 70%

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Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

N¹

2013

Enz Eng

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Proteolytic activity of two isolates of actinomycetes, Streptomyces albolongus and Streptomyces aburaviences was investigated on the basis of their ability to hydrolyze skimmed milk casein, egg albumin and gelatin. Both isolates were found to have potential for extracellular proteases production. Effects of culture conditions for the production of extracellular protease from S. albolongus and S. aburaviensis were determined. Highest protease yield from S. albolongus was obtained after 5 days of incubation with an initial p H 7, at static state when inoculated in medium composed of 1% glucose, 2% beef extract, 0.2% yeast extract, 0.1% KH 2 PO 4 , 0.3% K 2 HPO 4 , and trace MgSO 4 .7H 2 O. Optimum incubation conditions for S. abureviences were 4 days, with an initial medium p H 8 at shaking condition (100 rpm). S. aburaviences preferred 1.5% lactose and 1.5% tryptone as a carbon and nitrogen source. Both the isolates showed maximum protease yield at 37°C. The result of the present study might be helpful for largescale production of extracellular protease from these Streptomyces spp.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 70%

Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

N¹

2013

Enz Eng

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“…Caseinolytic activity was measured by the photometric method of Rahman et al (20). One unit (U) of protease activity is equivalent to 0.5 µg of tyrosine liberated by 1.0 ml of enzyme solution under the assay conditions.…”

Section: Enzyme Assaymentioning

confidence: 99%

Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization

Sangeetha

Geetha

Arulpandi³

2010

Braz. J. Microbiol.

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This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes.The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and solvents. The molecular weight of both the enzymes was determined as 40 kDa on SDS-PAGE. The concomitant production of protease and lipase and the purification of both the enzymes in a single mixture have industrial significance, as many industrial processes use both protease and lipase together.

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“…Trabalhando com rizóbios, Oliveira et al (2006b) concluíram que a atividade amilolítica de alguns isolados estão sujeitas às variações de pH do meio sólido, com as maiores atividades ocorrendo em pH 6,5. Apesar da importância da temperatura na produção de enzimas microbianas (Rahman et al 2005), dados sobre o efeito dessa variável na atividade enzimática de rizóbios em meio sólido são raros, ou mesmo inexistentes na literatura. O presente estudo objetivou avaliar semiquantitativamente, em meio sólido, a influência de diferentes pH e temperatura sobre a atividade amilolítica de rizóbios nativos da Amazônia Central.…”

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“…Quanto à temperatura, as maiores atividades foram registradas a 25 e 35 ºC (Tabela 1). Segundo Rahman et al (2005), ao provocar alterações nas propriedades físicas da membrana celular, a temperatura afeta, também, a síntese de enzimas extracelulares. De uma forma geral, esses resultados sugerem a natureza mesofílica das amilases produzidas pelos isolados.…”

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Influência do pH e temperatura sobre a atividade amilolítica de rizóbios isolados de solos da Amazônia

2010

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RESUMOPoucas são as informações referentes ao perfil enzimático de bactérias rizobiais. Em meio de cultura solidificado, foi conduzido um experimento em fatorial 7 x 3 x 3 para avaliar o efeito do pH (5,0; 6,5 e 8,0) e da temperatura (25, 35 e 42 o C) sobre a atividade amilolítica de sete isolados de rizóbio. As maiores atividades amilolíticas foram observadas em ambientes ácidos, com algumas bactérias também produzindo níveis significativos em pH 8,0. Entre as interações significativas (P<0,01), os isolados INPA R-110 e R-822 apresentaram máximas atividades em pH 5,0 e 25 o C, com o R-822 também sendo um bom produtor de amilase nas temperaturas de 35 e 42 o C. Em termos gerais, os isolados INPA R-110 e R-822 foram os melhores produtores de amilases, com atividades enzimáticas maiores do que 2,0. PALAVRAS-CHAVE:Amilases extracelulares, meio sólido, pH, temperatura Influence of pH and temperature on amylolytic activity of rhizobia isolated from Amazonian soils ABSTRACTInformation is scarce regarding the enzymatic profiles of rhizobia bacteria. On solid medium, a 7 x 3 x 3 factorial experiment was conducted to evaluate the effect of pH (5.0, 6.5 and 8.0) and temperature (25, 35 and 42 o C) on amylase activities of seven rhizobia strains. The highest enzymatic activities were found in acid environments, with some bacteria also producing significant levels at pH 8.0. Among the significant interactions (P<0.01), INPA strains R-110 and R-822 exhibited maximum activities at pH 5.0 and 25 o C, with the R-822 being also a good amylase producer at 35 and 42 o C. In general terms, INPA strains R-110 and R-822 were the best enzyme producers, with amylolytic enzymatic activities higher than 2.0.

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Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K

Cited by 132 publications

References 20 publications

Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization

Influência do pH e temperatura sobre a atividade amilolítica de rizóbios isolados de solos da Amazônia

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