Physical, structural, thermal and morphological characteristics of zeinquercetagetin composite colloidal nanoparticles

Sun, Cuixia; Liu, Fuguo; Yang, Jie; Yang, Wei; Yuan, Fang; Gao, Yanxiang

doi:10.1016/j.indcrop.2015.09.028

Cited by 43 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When the concentration of a bioactive compound increases in zein nanoparticles, the particle size increases. For instance, particle size of procyanidins has been reported in the range of 392 to 447 nm (Zou, Li, Percival, Bonard, & Gu, ), for retinol from 300.93 to 318.73 (Park, Park, & Kim, ), and for quercetin from 88.9 to 164 nm (Sun et al., ).…”

Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Quercetin‐Loaded Zein Nanoparticles by Electrospraying and Study of In Vitro Bioavailability

Rodríguez-Félix

Del‐Toro‐Sánchez

Cinco-Moroyoqui

et al. 2019

Journal of Food Science

View full text Add to dashboard Cite

Quercetin is a hydrophobic flavonoid with high antioxidant activity. However, for biological applications, the bioavailability of quercetin is low due to physiological barriers. For this reason, an alternative is the protection of quercetin in matrices of biopolymers as zein. The objective of this work was to prepare and characterize quercetin‐loaded zein nanoparticles by electrospraying and its study of in vitro bioavailability. The physicochemical parameters such as viscosity, density, and electrical conductivity of zein solutions showed a dependence of the ethanol concentration. In addition, rheological parameters demonstrated that solutions of zein in aqueous ethanol present Newtonian behavior, rebounding in the formation of nanoparticles by electrospraying, providing spherical, homogeneous, and compact morphologies, mainly at a concentration of 80% (v/v) of ethanol and of 5% (w/v) of zein. The size and shape of quercetin‐loaded zein nanoparticles were studied by transmission electron microscopy (TEM), observing that it was entrapped, distributed throughout the nanoparticle of zein. Analysis by Fourier transform‐infrared (FT‐IR) of zein nanoparticles loaded with quercetin revealed interactions via hydrogen bonds. The efficacy of zein nanoparticles to entrap quercetin was particularly high for all quercetin concentration evaluated in this work (87.9 ± 1.5% to 93.0 ± 2.6%). The in vitro gastrointestinal release of trapped quercetin after 240 min was 79.1%, while that for free quercetin was 99.2%. The in vitro bioavailability was higher for trapped quercetin (5.9%) compared to free quercetin (1.9%), than of gastrointestinal digestion. It is concluded, that the electrospraying technique made possible the obtention of quercitin‐loaded zein nanoparticles increasing their bioavailability. Practical Application This type of nanosystems can be used in the food and pharmaceutical industry. Quercetin‐loaded zein nanoparticles for its improvement compared to free quercetin can be used to decrease the prevalence of chronic degenerative diseases by increasing of the bioavailability of quercetin in the bloodstream. Other application can be as an antioxidant system in functional foods or oils to increase shelf life.

show abstract

Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Quercetin‐Loaded Zein Nanoparticles by Electrospraying and Study of In Vitro Bioavailability

Rodríguez-Félix

Del‐Toro‐Sánchez

Cinco-Moroyoqui

et al. 2019

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…When the mass ratio of zein to lecithin was 1:2, the α-helix and β-sheet contents were 45% and 16%. Sun et al [20] reported that the α-helix content decreased and β-sheet content increased with an increase of quercetagetin concentration, which revealed there existed the interaction between quercetagetin and zein.…”

Section: Resultsmentioning

confidence: 99%

“…Nephelometry experiments were performed in a HACH 2100N laboratory turbidimeter (Loveland, USA), and the turbidity of zein and lecithin composite colloidal dispersions was evaluated as described by Sun et al [20]. The optical apparatus was equipped with a tungsten-filament lamp with three detectors: a 90° scattered-light detector, a forward-scatter light detector, and a transmitted light detector.…”

Section: Methodsmentioning

confidence: 99%

The Interaction between Zein and Lecithin in Ethanol-Water Solution and Characterization of Zein–Lecithin Composite Colloidal Nanoparticles

Dai¹,

Sun²,

Wang³

et al. 2016

PLoS ONE

Self Cite

110

View full text Add to dashboard Cite

Lecithin, a naturally small molecular surfactant, which is widely used in the food industry, can delay aging, enhance memory, prevent and treat diabetes. The interaction between zein and soy lecithin with different mass ratios (20:1, 10:1, 5:1, 3:1, 2:1, 1:1 and 1:2) in ethanol-water solution and characterisation of zein and lecithin composite colloidal nanoparticles prepared by antisolvent co-precipitation method were investigated. The mean size of zein-lecithin composite colloidal nanoparticles was firstly increased with the rise of lecithin concentration and then siginificantly decreased. The nanoparticles at the zein to lecithin mass ratio of 5:1 had the largest particle size (263 nm), indicating that zein and lecithin formed composite colloidal nanoparticles, which might aggregate due to the enhanced interaction at a higher proportion of lecithin. Continuing to increase lecithin concentration, the zein-lecithin nanoparticles possibly formed a reverse micelle-like or a vesicle-like structure with zein in the core, which prevented the formation of nanoparticle aggregates and decreased the size of composite nanoparticles. The presence of lecithin significantly reduced the ζ-potential of zein-lecithin composite colloidal nanoparticles. The interaction between zein and lecithin enhanced the intensity of the fluorescence emission of zein in ethanol-water solution. The secondary structure of zein was also changed by the addition of lecithin. Differential scanning calorimetry thermograms revealed that the thermal stability of zein-lecithin nanoparticles was enhanced with the rise of lecithin level. The composite nanoparticles were relatively stable to elevated ionic strengths. Possible interaction mechanism between zein and lecithin was proposed. These findings would help further understand the theory of the interaction between the alcohol soluble protein and the natural small molecular surfactant. The composite colloidal nanoparticles formed in this study can broaden the application of zein and be suitable for incorporating water-insoluble bioactive components in functional food and beverage products.

show abstract

“…Fluorescence quenching is able to provide unique information about the interactions between drug molecules and proteins because of the sensitivity of intrinsic fluorescence to changes in the microenvironment around the protein. 23 Since zein shows intrinsic fluorescence attributable to amino acid residues containing aromatic moieties (phenylalanine, TRP, and TYR), fluorescence quenching might allow insights into the molecular interactions between FD and zein.…”

Section: Spectroscopic Analysis Uv Spectroscopymentioning

confidence: 99%

Complexes of Felodipine Nanoparticles With Zein Prepared Using a Dual Shift Technique

Ren

Xiong

et al. 2018

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

To improve the dissolution of felodipine, felodipine-zein complexes were prepared using a dual shift technique, with zein as both stabilizer and carrier. The complexes were characterized by particle size, zeta potential, morphology, crystalline properties, and release behavior. The complexes could be prepared in high yield and showed good redispersibility. The mean diameters of the felodipine particles in complexes were 150-300 nm, with negative zeta potentials of -30 to -25 mV after rehydration, and the particle sizes of the complexes were in the range 10-80 μm. The size of the felodipine nanoparticles incorporated into zein increased gradually with increasing drug content. Powder X-ray diffraction and differential scanning calorimetry indicated that felodipine in the complexes was markedly less crystalline than the pure drug. Both the rate and extent of dissolution of the complexes were significantly greater than those of the active pharmaceutical ingredient or physical mixtures. Spectroscopic analyses indicated that intermolecular interactions, especially hydrophobic interactions, are the major driving forces for the formation of the felodipine nanoparticles and contribute to the stabilization effect. This study provides a promising strategy for enhancing the dissolution rate of drugs using simplified preparation processes and showcases the design of zein-based oral delivery systems for bioactive components.

show abstract

Physical, structural, thermal and morphological characteristics of zeinquercetagetin composite colloidal nanoparticles

Cited by 43 publications

References 36 publications

Preparation and Characterization of Quercetin‐Loaded Zein Nanoparticles by Electrospraying and Study of In Vitro Bioavailability

Preparation and Characterization of Quercetin‐Loaded Zein Nanoparticles by Electrospraying and Study of In Vitro Bioavailability

The Interaction between Zein and Lecithin in Ethanol-Water Solution and Characterization of Zein–Lecithin Composite Colloidal Nanoparticles

Complexes of Felodipine Nanoparticles With Zein Prepared Using a Dual Shift Technique

Contact Info

Product

Resources

About