Physico-chemical Studies on the Interaction of Dendrimers with Lipid Bilayers. 1. Effect of Dendrimer Generation and Liposome Surface Charge

Roy, Biplab; Panda, Amiya Kumar; Parimi, Srinivas; Ametov, I. M.; Barnes, Timothy J.; Prestidge, Clive A.

doi:10.5650/jos.ess14081

Cited by 16 publications

(31 citation statements)

References 31 publications

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“…32 Initial size or turbidity increment and the attainment of maxima are due to the aggregation of liposomes assisted by dendrimers. 32 Dendrimers, being oppositely charged, adsorb on to the liposome surface. 27 The decrease in size and turbidity upon further addition of dendrimers was due to the formation of water-soluble dendrimer–liposome aggregates.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Earlier reports reveal that the activities follow the opposite trend G lower generation > G higher generation . 32 The lower the generation, the higher number of end groups become accessible for effective interactions. With increasing dendrimer generation, the end groups tend to back fold.…”

Section: Results and Discussionmentioning

confidence: 99%

“…With increasing dendrimer generation, the end groups tend to back fold. 32 Thus, for higher generation dendrimers, lesser number of end groups could effectively participate in the interaction process.…”

Section: Results and Discussionmentioning

confidence: 99%

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Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer–Liposome Aggregates

et al. 2018

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Interaction between negatively charged liposomes and cationic polyamidoamine dendrimers of different generations was investigated through size, zeta potential, turbidity, electron microscopy, atomic force microscopy, fluorescence spectroscopy, and calorimetric studies. Liposomes with the binary combination of 1,2-dipalmitoyl- sn -glycero-3-phosphatidylcholine (DPPC) + dihexadecyl phosphate, DPPC + 1,2-dimyristoyl- sn -glycero-3-phosphoglycerol, DPPC + 1,2-dipalmitoyl- sn -glycero-3-phosphate, and DPPC + 1,2-dipalmitoyl- sn -glycero-3-phosphoethanol were stable up to 60 days. The electrostatic nature of dendrimer–lipid bilayer interaction was evidenced through charge neutralization and subsequent reversal upon added dendrimer to liposome. Dendrimer–liposome interaction depended on its generation (5 > 4 > 3) in addition to the charge, head groups, and hydrocarbon chain length of lipids. Fluorescence anisotropy and differential scanning calorimetry studies suggest the fluidization of the bilayer, although the surface rigidity was enhanced by the added dendrimers. Thermodynamic parameters of the interaction processes were evaluated by isothermal titration and differential scanning calorimetric studies. The binding processes were exothermic in nature. The enthalpy of transition of the chain melting of lipids decreased systematically with increasing dendrimer concentration and generation. Dendrimer–liposome aggregates were nontoxic to healthy human blood cell, suggesting the potential of such aggregates as drug delivery systems.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

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Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer–Liposome Aggregates

et al. 2018

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“…We used two related biophysical techniques to study the binding behaviour of anionic PAMAM G4.5 and cationic PAMAM G5 to zwitterionic DPPC and anionic DPPG monolayers. To our knowledge, existing membrane studies of PAMAM dendrimers have been mainly focussing on cationic dendrimers [26,27,29] as due to their charge they have been proven good agents for drug [3,4] and gene delivery [7,8].…”

Section: Discussionmentioning

confidence: 99%

“…Biophysical and in vitro biological studies have been carried out to elucidate PAMAM-membrane interactions, primarily using cells [24,25], vesicles [26,27,28], and lipid bilayers [27,29,30,31]. For studies investigating the impact of lipid composition and solution properties on polymer binding to model membranes, lipid monolayers at the air/water interface are well-established physico-chemical models.…”

Section: Introductionmentioning

confidence: 99%

Biophysical studies in polymer therapeutics: the interactions of anionic and cationic PAMAM dendrimers with lipid monolayers

Wilde

Green

Sanders

et al. 2017

Journal of Drug Targeting

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Understanding how polymers interact with biological membranes is important for the development of polymer-based therapeutics and wider biomedical applications. Here, biophysical methods (surface pressure measurements, external reflection FTIR) have been used to investigate the interaction between PAMAM dendrimers (Generation 5 or 4.5) and anionic (DPPG) or zwitterionic (DPPC) model membranes. We observed a concentration-dependent binding behaviour of both PAMAM species to both model membranes; however, equivalent levels of penetration into DPPC monolayers required approximately 10-fold higher dendrimer concentrations than for penetration into DPPG monolayers. Overall, the anionic PAMAM G4.5 showed a slightly better penetration ability which could be caused by repulsive forces towards the lipid layers. In comparison, increasing concentration of cationic PAMAM G5 leads to saturation of adsorption at the anionic lipid surface before penetration into the lipid layer likely driven by electrostatic attraction. Our studies also showed that physiologically relevant concentrations of sodium chloride (144 mM) decreased PAMAM penetration into DPPG monolayers but did not significantly affect the dendrimer-DPPC interaction. These results provide an insight into the mechanism of interaction between charged dendritic polymers with a lipid interface and show that the nature of such interactions are affected by lipid headgroup, dendrimer charge and solution salinity.

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How Does Temperature Affect the Dynamics of SARS-CoV-2 M Proteins? Insights from Molecular Dynamics Simulations

2022

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Physico-chemical Studies on the Interaction of Dendrimers with Lipid Bilayers. 1. Effect of Dendrimer Generation and Liposome Surface Charge

Cited by 16 publications

References 31 publications

Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer–Liposome Aggregates

Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer–Liposome Aggregates

Biophysical studies in polymer therapeutics: the interactions of anionic and cationic PAMAM dendrimers with lipid monolayers

How Does Temperature Affect the Dynamics of SARS-CoV-2 M Proteins? Insights from Molecular Dynamics Simulations

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