Physicochemical, Antioxidant and Antimicrobial Properties of Electrospun Poly(ε-caprolactone) Films Containing a Solid Dispersion of Sage (Salvia officinalis L.) Extract

Abstract

In this study, novel active films made of poly(ε-caprolactone) (PCL) containing a solid dispersion of sage extract (SE) were developed by means of the electrospinning technique and subsequent annealing treatment. Initially, the antioxidant and antimicrobial potential of SE was confirmed. Thereafter, the effect of SE incorporation at different loading contents (5%, 10%, and 20%) on the physicochemical and functional properties of the films was evaluated. The films were characterized in terms of morphology, transparency, water contact angle, thermal stability, tensile properties, water vapor, and aroma barrier performances, as well as antioxidant and antimicrobial activities. Thin, hydrophobic films with good contact transparency were produced by annealing of the ultrathin electrospun fibers. Interestingly, the effect of SE addition on tensile properties and thermal stability of the films was negligible. In general, the water vapor and aroma permeability of the PCL-based films increased by adding SE to the polymer. Nevertheless, a strong 2,2-diphenyl-1-picrylhydrazyl (DPPH·) free radical scavenging ability, and a strong activity against foodborne pathogens Staphylococcus aureus and Escherichia coli were achieved by SE incorporation into PCL matrix. Overall, the obtained results suggest great potential of the here-developed PCL-based films containing SE in active food packaging applications with the role of preventing oxidation processes and microbial growth.

Electrospun Fibers and Sorbents as a Possible Basis for Effective Composite Wound Dressings

“…Despite the fact that PCL and PVP are polymers with no antimicrobial properties, our results showed that pure PCL in the form of fibers show slight antimicrobial activity for S. aureus and E. coli ( Figure S5); similar results were previously reported by Lagarón and his group [96]. Some evidence can be found that PCL and PVP are not ignored by bacterial cells and the growth is altered, after certain time bacteria recover their viability.…”

“…Ag-Si/Al 2 O 3 possesses antiseptic qualities and biocompatibility for biological tissues in animal experiments [41]. They also showed a bactericidal effect against Escherichia coli [96] and excellent bactericidal and fungicidal properties [105,106]. As mentioned in the results, all sorbents evaluated in this work present an increase in antimicrobial potency over time, being the most evident effect for P. aeruginosa.…”

“…In another study, electrospun fibers of pure PCL showed certain antimicrobial activity against E. coli (ATCC25922) with 16.66% drop of activity after 24 h of contact and with S. aureus (ATCC6538) with 13.33% drop of activity after 24 h of contact [89]. Finally, PCL films also showed certain reduction of bacterial forming units of E. coli and S. aureus [96].…”

“…The improvement of antimicrobial activity for PCL/PVP 85:15 scaffold compared with pure PCL fibers could be associated with the prolonged release of PVP. As observed in tumor cells, PVP could interfere with SOD and other antioxidant enzymes of bacterial and fungus to produce growth inhibition [96,98]. The faster dissolution of pure PVP fibers could be the reason not to observe the antimicrobial activity.…”

Improving polyphenolic thermal stability ofAristotelia Chilensisfruit extract by encapsulation within electrospun cyclodextrin capsules

“…In both techniques, an electrical potential is applied between a droplet of a matrix solution held at the end of a capillary tube and grounded target and when the applied electric field overcomes the surface tension of the droplet, a charged jet of solution is ejected (López de Dicastillo et al, ). Several works have already shown the effectiveness of encapsulation of active compounds, such as vanillin, curcumin, natural extracts, and essential oils, into different electrospun matrixes such as polymers and proteins (Arrieta, López de Dicastillo, Garrido, Roa, & Galotto., ; Kayaci & Uyar, ; Pérez‐Masiá et al, ; Rieger & Schiffman, ; Salevic, Prieto, Cabedo, Nedovic, & Lagaron, ; Torkamani, Syahariza, Norziah, Wan, & Juliano, ; Wang et al, ). Thus, this work presents the selection of a powerful extract of maqui fruit (MQE) based on its polyphenolic, antioxidant, antimicrobial, and anti‐tyrosinase properties to be further successfully encapsulated into electrospun hydroxypropyl‐β‐cyclodextrin (HPβCD) capsules.…”