Physicochemical characteristics and antibacterial effects of silver nanoparticles produced using the aqueous extract of Ilex paraguariensis

Silveira, Ariane Pandolfo; Bonatto, Cínthia Caetano; Lopes, C.A.P.; Rivera, Luis Miguel Ramirez; Silva, Luciano

doi:10.1016/j.matchemphys.2018.05.068

Cited by 18 publications

(9 citation statements)

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“…TEM results suggested that the size of the AgNPs increased with an increased amount of GM peels powder. This result is consistent with the previously reported work [40]. From the observation, low quantity of GM extract cannot prevent aggregation of the NPs, it was only sufficient for the reduction of Ag precursor.…”

Section: Discussionsupporting

confidence: 93%

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Bio-Mediated Synthesis and Characterisation of Silver Nanocarrier, and Its Potent Anticancer Action

et al. 2019

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Discovery of a potent drug nanocarrier is crucial for cancer therapy in which drugs often face challenges in penetrating efficiently into solid tumours. Here, biosynthesis of silver nanoparticles (AgNPs) using a waste material, Garcinia mangostana (GM) fruit peel extract is demonstrated. The best condition for AgNPs synthesis was with 0.5 g of peel extract, 7.5 mM silver nitrate at 45 °C, ~pH 4 for 16 h. The synthesized AgNPs were spherical and 32.7 ± 5.7 nm in size. To test its efficiency to be used as drug carrier, plant-based drug, protocatechuic acid (PCA) was used as a test drug. AgNPs loaded with PCA (AgPCA) resulted in 80% of inhibition at 15.6 µg/mL as compared to AgNPs which only killed 5% of HCT116 colorectal cells at same concentration. The IC50 of AgNPs and AgPCA for HCT116 were 40.2 and 10.7 µg/mL, respectively. At 15.6 µg/mL, AgPCA was not toxic to the tested colon normal cells, CCD112. Ag-based drug carrier could also potentially reduce the toxicity of loaded drug as the IC50 of PCA alone (148.1 µg/mL) was higher than IC50 of AgPCA (10.7 µg/mL) against HCT116. Further, 24-h treatment of 15.6 µg/mL AgPCA resulted in loss of membrane potential in the mitochondria of HCT116 cells and increased level of reaction oxygen species (ROS). These could be the cellular killing mechanisms of AgPCA. Collectively, our findings show the synergistic anticancer activity of AgNPs and PCA, and its potential to be used as a potent anticancer drug nanocarrier.

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Section: Discussionsupporting

confidence: 93%

“…The presence of C-H bond was detected at the region of 2914 cm −1 (GM) and 2929 cm −1 (AgNPs), suggesting that xanthone and other compounds in the peels extract were present [40]. The region of 1700 cm −1 shows the presence of C = O stretching for both GM and AgNPs [41].…”

Section: Resultsmentioning

confidence: 99%

Bio-Mediated Synthesis and Characterisation of Silver Nanocarrier, and Its Potent Anticancer Action

et al. 2019

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“…Furthermore, AgNPs release Ag + ions via oxidation increasing the generation of reactive oxidative species, which damage cell components and cause cell death (Dogru et al, 2017; Dong et al, 2019). Our results also showed that a greater concentration of AgNPs is needed to inhibit and kill gram‐positive strains compared to gram‐negative strains and are consistent with results of other authors (Alfuraydi et al, 2019; Silveira et al, 2018). This could be due to the fact that the cell wall of gram‐positive bacteria, which is composed of a rigid thick multilayer of peptidoglycan, prevents NPs from entering the cell.…”

Section: Discussionsupporting

confidence: 93%

Biologically synthesized silver nanoparticles, mediated by Bothriochloa laguroides , inhibit biofilm formation and eradicate mature biofilm of Yersinia enterocolitica and Staphylococcus aureus

Toranzo

Bustos

Ortega

et al. 2021

J of Applied Microbiology

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Aims To phytosynthesize silver nanoparticles (AgNPs) and determine their antibacterial and antibiofilm capacity against gram‐positive and gram‐negative bacterial strains. Methods and Results AgNPs were synthesized using Bothriochloa laguroides aqueous extract as reducing and stabilizing agent. After characterization, a phytochemical screening to the extract and the AgNPs was performed. Antibacterial activity, inhibition and eradication of biofilms against Staphylococcus aureus and Yersinia enterocolitica strains were tested. Spherical AgNPs with an average size of 8 nm were obtained. Tannins, flavonoids, carbohydrates, proanthocyanidins, anthocyanins and saponins were identified in aqueous extract; meanwhile, only carbohydrates were identified in AgNPs. The MIC and MBC were determined at pmol L−1 levels for all tested strains. Furthermore, AgNPs inhibited more than 90% of biofilms formation and eradicated more than 80% of mature biofilms at concentrations higher than MIC. Conclusions The AgNPs obtained in this study inhibited planktonic and sessile growth, and eradicated mature biofilms of pathogenic bacterial strains at very low concentrations. Significance and Impact of Study The current study showed the promising potential of AgNPs as antibiofilm agents opening the way for the future development of a new class of antibacterial products.

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“…Several studies successfully reported the biosynthesis of AgNPs using plant materials as reducing agents, such as Anacardium othonianum , Aloe vera , Emblica officinalis , Artemisia absinthium , Pelargonium sp. and Ilex paraguariensis as a result of the eco‐friendly and simple processes required for their synthesis.…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of silver nanoparticles using tomato leaf extract and their entrapment in chitosan nanoparticles to control bacterial wilt

et al. 2019

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BACKGROUND: Silver nanoparticles (AgNPs), particularly those entrapped in polymeric nanosystems, have arisen as options for managing plant bacterial diseases. Among the biopolymers useful for the entrapment of AgNPs, chitosan is promising because of its low cost, good biocompatibility, antimicrobial properties and biodegradability. The present study aimed: (i) to greenly-synthesize AgNPs using different concentrations of aqueous extract of tomato leaves followed by entrapment of AgNPs with chitosan (CH-AgNPs); (ii) to characterize the optical, structural and biological properties of the nanosystems produced; (iii) to evaluate the antimicrobial activities of AgNPs and nanomaterials; and (iv) to assess the effectiveness of AgNPs and nanomaterials for controlling tomato bacterial wilt caused by Ralstonia solanacearum. RESULTS: Spherical and oval AgNPs had incipient colloidal instability, although the concentration of the tomato leaf extractinfluenced both size (< 87 nm) and the polydispersity index. Nanomaterials (< 271 nm in size) were characterized by a highly stable matrix of chitosan containing polydisperse AgNPs. Free AgNPs and CH-AgNPs were stable for up to 30 days, with no significant alteration in physicochemical parameters. The AgNPs and nanomaterials had antibacterial activity and decreased bacterial growth at micromolar concentrations after 48 h. Morphological changes in R. solanacearum cells were observed after treatment with CH-AgNPs. The application of CH-AgNPs at 256 mol L −1 reduced the incidence of bacterial wilt in a partially resistant tomato genotype but not in the susceptible line. CONCLUSION: Greenly-synthesized chitosan-derived nanomaterials containing AgNPs produced with leaf extracts from their own species appear to comprise a promising and sustainable alternative in an integrated management approach aiming to reduce the yield losses caused by bacterial wilt. Synthesis of chitosan nanoparticlesAgNPs immersed in a polymeric matrix of chitosan were produced using the ionic gelation method with the addition of sodium J Sci Food Agric 2019; 99: 4248-4259 /jsfa FTIR FTIR measurements were carried out to identify the possible functional groups present in biomolecules from S. lycopersicum leaf extract and their role in the synthesis of AgNPs, as well as the role of J Sci Food Agric 2019; 99: 4248-4259 Figure 3. Transmission electron micrographs of AgNPs synthesized using 5 mg mL −1 tomato leaf extract (5AgNPs) (A) or 10 mg mL −1 tomato leaf extract (10AgNPs) (B), nanomaterial derived from 5AgNPs associated with chitosan (5CH-AgNPs) (C), nanomaterial derived from 10AgNPs associated with chitosan (10CH-AgNPs) (D), dry diameter assessed by TEM of AgNPs and chitosan-based nanomaterials (E) and hydrodynamic diameter dispersion of nanoparticles and nanomaterials (F). Scale bars = 20 nm. J Sci Food Agric 2019; 99: 4248-4259 CONCLUSIONSNanotechnology is a promising area with respect to the development of products for controlling bacterial plant pathogens. AgNPs alone and AgNPs-coated/entrapped with chitosa...

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Physicochemical characteristics and antibacterial effects of silver nanoparticles produced using the aqueous extract of Ilex paraguariensis

Cited by 18 publications

References 79 publications

Bio-Mediated Synthesis and Characterisation of Silver Nanocarrier, and Its Potent Anticancer Action

Bio-Mediated Synthesis and Characterisation of Silver Nanocarrier, and Its Potent Anticancer Action

Biologically synthesized silver nanoparticles, mediated by Bothriochloa laguroides , inhibit biofilm formation and eradicate mature biofilm of Yersinia enterocolitica and Staphylococcus aureus

Green synthesis of silver nanoparticles using tomato leaf extract and their entrapment in chitosan nanoparticles to control bacterial wilt

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