Physicochemical characteristics and cytotoxic effect of the methanolic extract of Croton heliotropiifolius Kunth (Euphorbiaceae)

Silva, JÃ©ssica de Andrade Gomes; Silva, Gibbelly Cavalcante da; Silva, Marília Grasielly de Farias; Silva, Vanessa Farias da; Aguiar, Jaciana dos Santos; Silva, Teresinha GonÃ§alves da; Leite, Sã nia Pereira

doi:10.5897/ajpp2017.4798

Cited by 9 publications

(1 citation statement)

References 15 publications

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“…In comparison, phosphate buffer (pH 7.4) presented an inhibition rate of 20.30 and 12.95% for RAW 264.7 and L929, respectively. According to Gomes Silva et al (2017), inhibition rates of 1-20% indicate an absence of inhibitory activity. Therefore, the biosurfactant has no cytotoxic potential regarding the strains studied.…”

Section: Cytotoxic Potential Of Biosurfactantmentioning

confidence: 99%

Potential Food Application of a Biosurfactant Produced by Saccharomyces cerevisiae URM 6670

Ribeiro

Guerra

Sarubbo

2020

Front. Bioeng. Biotechnol.

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Biosurfactants have aroused considerable interest due to the possibility of acquiring useful products that are tolerant to processing techniques used in industries. Some yeasts synthesize biosurfactants that offer antioxidant activity and thermal resistance and have no risk of toxicity or pathogenicity, demonstrating potential use in food formulations. The aim of the present study was to assess the use of a biosurfactant produced by Saccharomyces cerevisiae URM 6670 to replace egg yolk in a cookie formulation. The yeast was grown in a medium containing 1% waste soybean oil and 1% corn steep liquor. The biosurfactant was isolated using a novel method and was structurally characterized using FT-IR, NMR, and GC/FID. Thermal stability was determined using thermogravimetry (TG)/differential scanning calorimetry (DSC) and antioxidant activity was investigated using three methods. Cytotoxicity tests were performed using the MTT assay with mouse fibroblast and macrophage lines. In the final step, the biosurfactant was incorporated into the formulation of a cookie dough replacing egg yolk. The physical properties and texture profile were analyzed before and after baking. The surface and interfacial tensions of the culture medium after the production process were 26.64 ± 0.06 and 9.12 ± 0.04 mN/m, respectively, and the biosurfactant concentration was 5.84 ± 0.17 g/L after isolation. In the structural characterization by NMR and FT-IR, the biosurfactant from S. cerevisiae exhibited a glycolipid structure, with the fatty acid profile revealing a high percentage of linoleic acid (50.58%). The thermal analysis demonstrated stability at the industrial application temperature, with the negligible loss of mass at temperatures of up to 200 • C. The biosurfactant was non-toxic to the fibroblast and macrophage cell lines, with cell inhibition less than 15%. The incorporation of the biosurfactant into the cookie dough did not alter the physical or physicochemical properties of the product after baking. In the analysis of the texture profile before baking, the substitution of egg yolk with the biosurfactant did not alter the properties of firmness, cohesiveness, or elasticity compared to the standard formulation. Therefore, the biosurfactant produced by S. cerevisiae URM 6670 has potential applications in the food industry as a replacement for egg yolk.

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Section: Cytotoxic Potential Of Biosurfactantmentioning

confidence: 99%

Potential Food Application of a Biosurfactant Produced by Saccharomyces cerevisiae URM 6670

Ribeiro

Guerra

Sarubbo

2020

Front. Bioeng. Biotechnol.

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In vitro evaluation of cytotoxic potential of essential oil extracted from leaves of Croton heliotropiifolius Kunth in human tumor cells

Cavalcanti,

Magalhães,

Rocha

et al. 2023

Journal of Toxicology and Environmental Health, Part A

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Effects of lipopeptide biosurfactants on clinical strains of Malassezia furfur growth and biofilm formation

et al. 2021

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Lipopeptide biosurfactants (LBs) are biological molecules with low toxicity that have aroused growing interest in the pharmaceutical industry. Their chemical structure confers antimicrobial and antibiofilm properties against different species. Despite their potential, few studies have demonstrated their capability against Malassezia spp., commensal yeasts which can cause dermatitis and serious infections. Thus, the aim of this study was to evaluate the antifungal activity of biosurfactants produced by new strains of Bacillus subtilis TIM10 and B. vallismortis TIM68 against M. furfur and their potential for removal and inhibition of yeast biofilms. Biosurfactants were classified as lipopeptides by FTIR, and their composition was characterised by ESI-Q-TOF/MS, showing ions for iturin, fengycin, and surfactin, with a greater abundance of surfactin. Through the broth microdilution method, both biosurfactants inhibited the growth of clinical M. furfur strains. Biosurfactant TIM10 showed greater capacity for growth inhibition, with no statistical difference compared to those obtained by the commercial antifungal fluconazole for M. furfur 153DR5 and 154DR8 strains. At minimal inhibitory concentrations (MIC-2), TIM10 and TIM68 were able to inhibit biofilm formation, especially TIM10, with an inhibition rate of approximately 90%. In addition, both biosurfactants were able to remove pre-formed biofilm. Both biosurfactants showed no toxicity against murine fibroblasts, even at concentrations above MIC-2. Our results show the effectiveness of LBs in controlling the growth and biofilm formation of M. furfur clinical strains and highlight the potential of these agents to compose new formulations for the treatment of these fungi.

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Physicochemical characteristics and cytotoxic effect of the methanolic extract of Croton heliotropiifolius Kunth (Euphorbiaceae)

Abstract: Croton heliotropiifolius

Cited by 9 publications

References 15 publications

Potential Food Application of a Biosurfactant Produced by Saccharomyces cerevisiae URM 6670

Potential Food Application of a Biosurfactant Produced by Saccharomyces cerevisiae URM 6670

In vitro evaluation of cytotoxic potential of essential oil extracted from leaves of Croton heliotropiifolius Kunth in human tumor cells

Effects of lipopeptide biosurfactants on clinical strains of Malassezia furfur growth and biofilm formation

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