Physicochemical Characterization, Standardization and In vitro Determination of Radical Scavenging Activity of Zereshk-e- Saghir, A Traditional Preparation, and Its Ingredients

Sarhadynejad, Zarrin; Pardakhty, Abbas; Mandegary, Ali; Afsharypuor, Suleiman; Sharififar, Fariba

doi:10.5530/jyp.2017.9.44

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…The free radical scavenging activity of compounds 1 and 2 was measured based on DPPH scavenging activity. [38] The reaction solution consisted of 50 μL of different concentrations of compounds 1 and 2 (0-500g/mL) and 150 μL of DPPH solution in methanol (0.15 mM). After shaking the mixtures for 60 s, they were incubated for 30 min at room temperature while shielded from light.…”

Section: Dpph Assaymentioning

confidence: 99%

Evaluation of Cytotoxic and Antioxidant Effect of Guaianolid Sesquiterpenes from Ferula hindukushensis

Mehrabani,

Iranshahi,

Mehrabani

et al. 2023

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Ferula species have been recognized as crucial medicinal plants that are known for producing a variety of sesquiterpenes, such as sesquiterpene lactones and sesquiterpene coumarins. However, guaianolide sesquiterpenes, which are sesquiterpene lactones that contain an alpha‐methylene‐gamma‐lactone moiety, are rare in Ferula spp. Ferula hindukushensis, an endemic species found in Afghanistan, Pakistan, and Iran, belongs to the Ferula genus. In this study, the normal column chromatography technique was employed to fractionate the dichloromethane extract of F. hindukushensis. The structures of two guaianolide sesquiterpenes were identified using various nuclear magnetic resonance (NMR) spectra, such as 13C NMR, 1H NMR, and heteronuclear multiple bond correlation (HMBC), and were compared with the literature interpretation to confirm their structural characteristics. Moreover, the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay was employed to examine the cytotoxicity of guaianolide compounds on various cell lines, including MCF‐7, 3T3, HT‐29, MCF‐10, PC12, and A549. The antioxidant effects of these compounds were evaluated using ferric reducing antioxidant power (FRAP) and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assays. The findings indicated that the antioxidant activity of the compounds was lower than that of vitamin C at the same concentration. The cytotoxic effects of the compounds varied depending on the cell types. Nevertheless, except for HT‐29, a concentration lower than 125 μg/mL was found to be non‐toxic for all cell lines.

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Section: Dpph Assaymentioning

confidence: 99%

Evaluation of Cytotoxic and Antioxidant Effect of Guaianolid Sesquiterpenes from Ferula hindukushensis

Mehrabani,

Iranshahi,

Mehrabani

et al. 2023

ChemistrySelect

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“…Due to increased global interest in plant research with a traditional origin and their therapeutic potential, using scientific approaches is extremely needed to evaluate their properties, safety, therapeutic effects, and their validity (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

Comparing the Effect of Dill Seed Vaginal Cream, with a Persian Traditional Base, and 1% Clotrimazole Vaginal Cream on Vulvovaginal Candidiasis: A Double-Blind, Randomized Clinical Trial

Sarhadinejad¹,

Tajadini²,

Tansaz³

et al. 2018

Iran Red Crescent Med J

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Background: Vulvovaginal candidiasis (VVC) is a very common debility in gynecology clinics. Despite access to several antifungal agents, VVC is still a challenge; therefore, presenting a novel antifungal agent, especially with a traditional origin, is of interest and demand. Objectives:The current study aimed at comparing the efficacy of Anethum graveolens L. (dill) vaginal cream with that of 1% Clotrimazole vaginal cream to treat VVC. Methods:A prospective, single-center, randomized, double-blind clinical trial was performed. In the current study, married females aged 18 -65 years, with probable vulvovaginal candidiasis were enrolled for primary evaluation. After VVC confirmation by the sniff test, the patients were allocated into three groups with regard to blocked randomization, and inclusion and exclusion criteria. The first group (n = 59) was treated with 1% Clotrimazole vaginal cream; the second (n = 60) with a combination of dill seed essential oil and dried aqueous extract vaginal cream (TEE); and the last (n = 56) with dill seed essential oil vaginal cream (EO) for seven nights. After 10 days, the therapeutic effects were assessed.Results: After the intervention, itching decreased to 30.5%, 3.3%, and 12.5% in the Clotrimazole, TEE, and EO groups, respectively; the sniff test also decreased to 33.9%, 13.3%, and 12.5%, respectively. Although a statistically significant difference, according to itching and sniff test, was observed among the three groups (P < 0.001, P = 0.005), there was no significant difference between TEE and EO (P = 0.06). Conclusions:The current study showed that the herbal preparation could be used as an alternative antifungal agent for vulvovaginal Candidiasis.

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“…8 Therefore, this study also investigates the chemical profile using TLC and LC-MS method. The extractable matter, TLC 9 and LC-MS 10 fingerprint can be used as a reference for standardization of quality of herbal medicines. 9,10 …”

Section: Introductionmentioning

confidence: 99%

“…The extractable matter, TLC 9 and LC-MS 10 fingerprint can be used as a reference for standardization of quality of herbal medicines. 9,10 …”

Section: Introductionmentioning

confidence: 99%

Influence of Extraction Parameters on the Yield, Phytochemical, TLC-Densitometric Quantification of Quercetin, and LC-MS Profile, and how to Standardize Different Batches for Long Term from Ageratum conyoides L. Leaves

Tambunan

Bahtiar²,

Tjandrawinata³

2017

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Physicochemical Characterization, Standardization and In vitro Determination of Radical Scavenging Activity of Zereshk-e- Saghir, A Traditional Preparation, and Its Ingredients

Cited by 7 publications

References 14 publications

Evaluation of Cytotoxic and Antioxidant Effect of Guaianolid Sesquiterpenes from Ferula hindukushensis

Evaluation of Cytotoxic and Antioxidant Effect of Guaianolid Sesquiterpenes from Ferula hindukushensis

Comparing the Effect of Dill Seed Vaginal Cream, with a Persian Traditional Base, and 1% Clotrimazole Vaginal Cream on Vulvovaginal Candidiasis: A Double-Blind, Randomized Clinical Trial

Influence of Extraction Parameters on the Yield, Phytochemical, TLC-Densitometric Quantification of Quercetin, and LC-MS Profile, and how to Standardize Different Batches for Long Term from Ageratum conyoides L. Leaves

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