Physicochemical, Pharmacologic, and in Vitro Cellular Effects of Loading Collagen Membranes with Zoledronic Acid

Liao, Jian; Meng, Yang; Zhai, Jun-Jiang; Wen, Cai; Ten, MinHua; Tian, Ai Hua; Wang, Yong; Liang, Xing

doi:10.11607/jomi.3030

Cited by 8 publications

(12 citation statements)

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“…Although collagen barrier membranes were proposed as carriers for zoledronate and growth factors due to their net effects, studies assessing the release kinetic are limited to antibiotics and zoledronate. [20][21][22] Our observations are in line with the burst release of zoledronate from 1 and 2 layered collagen barrier membranes and data from prolyl hydroxylase, vitamins, and growth factors that were adsorbed onto bone substitute materials. 20,23,24 However, the membranes release prolyl hydroxylase inhibitors within the first 6 hours which is faster than found with vitamins and growth factors on bone substitute materials.…”

Section: Discussionsupporting

confidence: 84%

“…[20][21][22] Our observations are in line with the burst release of zoledronate from 1 and 2 layered collagen barrier membranes and data from prolyl hydroxylase, vitamins, and growth factors that were adsorbed onto bone substitute materials. 20,23,24 However, the membranes release prolyl hydroxylase inhibitors within the first 6 hours which is faster than found with vitamins and growth factors on bone substitute materials. 23,24 However, this release kinetic is in line with the data from zoledronate from collagen barrier membranes where the highest release is found within the first 6 h. 20 That only one dose of prolyl hydroxylase inhibitors was assessed is a clear limitation of this study.…”

Section: Discussionsupporting

confidence: 84%

“…Studies with the double-layered BioGide Õ and the single-layered collagen barrier membrane BME-10X composed of bovine collagen I showed no difference regarding the release of bisphosphonates. 20 These data suggest that it is unlikely that monolayered collagen membranes show a different release kinetic.…”

Section: Discussionmentioning

confidence: 93%

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Release kinetics of prolyl hydroxylase inhibitors from collagen barrier membranes

2014

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Collagen barrier membranes are used in guided tissue regeneration to support healing. This strategy, however, relies on the healing capacity of the tissue. Pharmacological inhibitors of prolyl hydroxylases can support regeneration by enhancing angiogenesis and are therefore a promising tool for periodontology. Here we evaluate the release kinetics of the prolyl hydroxylase inhibitors dimethyloxalylglycine and L-mimosine from collagen barrier membranes. Dimethyloxalylglycine and L-mimosine were lyophilized onto the collagen barrier membranes. The morphology of the collagen barrier membranes was analysed using scanning electron microscopy. The release of prolyl hydroxylase inhibitors was assessed by colorimetric and spectroscopic methods. Their ability to induce a cellular response was assessed in bioassays with gingival and periodontal ligament fibroblasts based on vascular endothelial growth factor production, proliferation, and metabolic activity of the cells. We found that loading of collagen barrier membranes with prolyl hydroxylase inhibitors did not change the overall membrane morphology. Assessment of the release kinetics by direct measurements and based on vascular endothelial growth factor production showed that supernatants obtained from the collagen barrier membranes in the first 6 hours had a sufficient level of prolyl hydroxylase inhibitors to induce vascular endothelial growth factor production. A similar kinetic was found when cell proliferation was assessed. Changes in metabolic activity did not reach the level of significance in the MTT assay. In conclusion, collagen barrier membranes can release prolyl hydroxylase inhibitors thereby increasing the pro-angiogenic capacity of periodontal cells in vitro. These findings provide the basis for preclinical studies to evaluate the regenerative capacity of prolyl hydroxylase inhibitors in periodontology and oral surgery.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 84%

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Release kinetics of prolyl hydroxylase inhibitors from collagen barrier membranes

2014

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“…CBM with a different structure might show different release kinetics. Comparison of BioGide® with the single-layered CBM BME-10X composed of bovine collagen I revealed different release kinetics of bisphosphonates from these membranes [4]. Clearly, platelets release other factors besides PDGF-BB and TGFβ1 that induce mitogenic effects, including other PDGF isoforms such as PDGF-AB and other signalling molecules [37].…”

Section: Discussionmentioning

confidence: 99%

“…When healing is compromised, therapeutic approaches based on biologicals can support regeneration. CBM represent a promising carrier for bioactive molecules that stimulate the healing capacity as they are placed in the interface between soft and hard tissue defects where they can release factors and pharmaceuticals to target cells of both tissues [4, 5]. Platelets have been investigated for their capacity to stimulate oral tissue regeneration based on their key role in the wound healing cascade and their capacity to modulate inflammation [6–9].…”

Section: Introductionmentioning

confidence: 99%

Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

et al. 2017

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BackgroundPlatelet preparations can stimulate the healing process and have mitogenic properties. We hypothesized that collagen barrier membranes (CBM), clinically used in guided bone regeneration and guided tissue regeneration, can serve as carriers for platelet secretome.MethodsSecretome was generated from washed platelets and unwashed platelets (washed/unwashed PSEC) and lyophilized onto CBM. Overall appearance of CBM was evaluated by scanning electron microscopy. The impact of PSEC on cell attachment was measured based on fluorescence microscopy with DiI-labeled cells. To assess the release kinetics, supernatants of CBM were collected and medium was replaced at hour 1–48. The mitogenic effect was evaluated with periodontal fibroblasts. Furthermore, the release of total protein, platelet-derived growth factor (PDGF)-BB, and transforming growth factor (TGF) β1 was measured.ResultsCBM overall appearance and cell attachment was not modulated by PSEC. Supernatants taken after one hour induced a mitogenic response in fibroblasts and showed the highest levels of total protein, TGFβ1 and PDGF-BB. These effects decreased rapidly in subsequent supernatants. While supernatants of CBM loaded with unwashed PSEC induced a stronger mitogenic response than supernatants of CBM loaded with washed PSEC this difference between the PSEC preparations was not observed when cells were seeded on 48–hours-washed CBM.ConclusionsCBM release platelet-derived factors in continuously declining release kinetics.

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Collagen barrier membranes do not adsorb hypoxia mimetic activity‐Activity of gingival fibroblasts cultured directly on collagen barrier membranes loaded with hypoxia mimetic agents

et al. 2017

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Hypoxia-based strategies for applications in oral surgery and periodontology have been proposed where collagen barrier membranes (CBM) are loaded with hypoxia mimetic agents (HMA) to induce a pro-angiogenic response. While it was found that CBM release HMA, it remained unclear if CBM adsorb HMA activity. Here we evaluated the response of oral cells cultured on CBM, supplemented with the HMA dimethyloxalylglycine (DMOG), desferrioxamine (DFO), and l-mimosine (l-MIM). Gingival fibroblasts (GF) were cultured on unwashed CBM as well as on CBM that had been washed with serum-free medium for 48 hours. The pro-angiogenic response was measured based on vascular endothelial growth factor (VEGF) production. Viability and proliferation were assessed based on MTT and BrdU assays. We found that GF seeded onto CBM loaded with DFO and l-MIM, but not DMOG, showed an increase in VEGF to 6.1-fold and 7.7-fold compared to unloaded CBM, respectively. Cells remained vital, but a trend for decreased proliferation was observed on DMOG and DFO-loaded CBM which did not reach the level of significance. Evaluation of washed CBM revealed no difference between the unloaded CBM and CBM supplemented with DMOG, DFO, or l-MIM. In conclusion, our results suggest that CBM do not adsorb hypoxia mimetic activity but release HMA within the first hours. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 874-879, 2018.

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Physicochemical, Pharmacologic, and in Vitro Cellular Effects of Loading Collagen Membranes with Zoledronic Acid

Cited by 8 publications

References 0 publications

Release kinetics of prolyl hydroxylase inhibitors from collagen barrier membranes

Release kinetics of prolyl hydroxylase inhibitors from collagen barrier membranes

Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

Collagen barrier membranes do not adsorb hypoxia mimetic activity‐Activity of gingival fibroblasts cultured directly on collagen barrier membranes loaded with hypoxia mimetic agents

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