Physiochemical properties of various polymer substrates and their effects on microchip electrophoresis performance

Shadpour, Hamed; Musyimi, Harrison K.; Chen, Jifeng; Soper, Steve Allan

doi:10.1016/j.chroma.2005.08.083

Cited by 159 publications

(146 citation statements)

References 56 publications

Supporting

Mentioning

142

Contrasting

Unclassified

Order By: Relevance

“…Glass, Plexiglas (polymethylmethacrylate), and polyvinyl chloride (PVC) plates (30 by 30 cm), with different degrees of surface hydrophilicity (glass Ͼ Plexiglas Ͼ PVC) according to water contact angle measurements obtained previously (26,50), were used as test surfaces. So that data could be compared, a construct similar to that used in a previous experiment (15) was employed to deploy the surfaces in seawater at a depth of 1 m below the water surface near Wheat Island off the Qingdao coast (36.31°N, 120.25°E) on 23 May 2005.…”

Section: Methodsmentioning

confidence: 99%

Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters

Dang

Chen

et al. 2008

Appl Environ Microbiol

357

279

View full text Add to dashboard Cite

Bacterial surface colonization is a universal adaptation strategy in aquatic environments. However, neither the identities of early colonizers nor the temporal changes in surface assemblages are well understood. To determine the identities of the most common bacterial primary colonizers and to assess the succession process, if any, of the bacterial assemblages during early stages of surface colonization in coastal water of the West Pacific Ocean, nonnutritive inert materials (glass, Plexiglas, and polyvinyl chloride) were employed as test surfaces and incubated in seawater off the Qingdao coast in the spring of 2005 for 24 and 72 h. Phylogenetic analysis of the 16S rRNA gene sequences amplified from the recovered surface-colonizing microbiota indicated that diverse bacteria colonized the submerged surfaces. Multivariate statistical cluster analyses indicated that the succession of early surface-colonizing bacterial assemblages followed sequential steps on all types of test surfaces. The Rhodobacterales, especially the marine Roseobacter clade members, formed the most common and dominant primary surface-colonizing bacterial group. Our current data, along with previous studies of the Atlantic coast, indicate that the Rhodobacterales bacteria are the dominant and ubiquitous primary surface colonizers in temperate coastal waters of the world and that microbial surface colonization follows a succession sequence. A conceptual model is proposed based on these findings, which may have important implications for understanding the structure, dynamics, and function of marine biofilms and for developing strategies to harness or control surface-associated microbial communities.

show abstract

Section: Methodsmentioning

confidence: 99%

Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters

Dang

Chen

et al. 2008

Appl Environ Microbiol

357

279

View full text Add to dashboard Cite

show abstract

“…It seems that the mobility decrease after a protein treatment is a common feature of many plastics. 10,22,37 The generally accepted explanation of this phenomenon is given in monograph. 38 In a stronger electrolyte, the counter-ions from the electrolyte firmly bind ͑the counter-ions are thus immobile͒ by the electrostatic interaction to the ionized groups of proteins that then behave as electroneutral molecules.…”

Section: E Effective Electric Charge Of Bsamentioning

confidence: 99%

“…Because PDMS microchips find their use in a variety of bioapplications, a few papers were aimed at the electro-osmotic transport of buffers containing biomacromolecules. 6,10,22 These molecules often interact with the PDMS surface due to hydrophobic interactions generally resulting in a decrease of EOF intensity. For instance, the presence of bovine serum albumin ͑BSA͒ in transported electrolytes decreased the electro-osmotic velocity in microsystems made from twelve different plastic substrates.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the presence of bovine serum albumin ͑BSA͒ in transported electrolytes decreased the electro-osmotic velocity in microsystems made from twelve different plastic substrates. 10 Zhou et al 22 evaluated the EOF mobility in a BSA treated PDMS microchannel to be 1.7ϫ 10 −8 m 2 V −1 s −1 . We summarized some available data on the electroosmotic mobilities in various PDMS devices in Table I.…”

Section: Introductionmentioning

confidence: 99%

“…Current monitoring a͒ technique is one of the most exploited; [6][7][8][9][10][11][12][13] however, other techniques such as amperometric detection method, 14 cyclic voltametry, 15 imaging of a caged fluorescent dye, 16,17 and particle tracking technique 18 were also employed.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Study on surface properties of PDMS microfluidic chips treated with albumin

Schrott¹,

Slouka²,

Červenka³

et al. 2009

Biomicrofluidics

View full text Add to dashboard Cite

Electrokinetic properties and morphology of PDMS microfluidic chips intended for bioassays are studied. The chips are fabricated by a casting method followed by polymerization bonding. Microchannels are coated with 1% solution of bovine serum albumin ͑BSA͒ in Tris buffer. Albumin passively adsorbs on the PDMS surface. Electrokinetic characteristics ͑electro-osmotic velocity, electro-osmotic mobility, and zeta potential͒ of the coated PDMS channels are experimentally determined as functions of the electric field strength and the characteristic electrolyte concentration. Atomic force microscopy ͑AFM͒ analysis of the surface reveals a "peak and ridge" structure of the protein layer and an imperfect substrate coating. On the basis of the AFM observation, several topologies of the BSA-PDMS surface are proposed. A nonslip mathematical model of the electro-osmotic flow is then numerically analyzed. It is found that the electrokinetic characteristics computed for a channel with the homogeneous distribution of a fixed electric charge do not fit the experimental data. Heterogeneous distribution of the fixed electric charge and the surface roughness is thus taken into account. When a flat PDMS surface with electric charge heterogeneities is considered, the numerical results are in very good agreement with our experimental data. An optimization analysis finally allowed the determination of the surface concentration of the electric charge and the degree of the PDMS surface coating. The obtained findings can be important for correct prediction and possibly for robust control of behavior of electrically driven PDMS microfluidic chips. The proposed method of the electro-osmotic flow analysis at surfaces with a heterogeneous distribution of the surface electric charge can also be exploited in the interpretation of experimental studies dealing with protein-solid phase interactions or substrate coatings.

show abstract

Electrozone Sensing Goes Nano

Figueiredo

Ferreira

Campos

2015

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Recent advances in nanopore‐based technologies and microelectronics allowed the resurgence of Coulter counter‐based techniques. Known collectively as resistive pulse sensing, this technique is now capable of characterizing nanoscale objects, such as nanoparticles, viruses, DNA, and other polymers, while keeping the main attractions of the classical versions: simplicity, sensitivity and resolution, and single‐object readout. Besides an accurate characterization of both size and concentration of the nanoparticles in their natural environment, additional information about particle surface charge is currently possible in an individual basis. Furthermore, efforts have been made to integrate the nanopores in microfluidic systems with the inherent advantages in terms of portability and cost as well as the ability to integrate multiple functions. This survey aims to review the progress in resistive pulse sensing toward the characterization of submicron particles, with special emphasis on nanopore design (natural and synthetic) and on lab‐on‐a‐chip devices.

show abstract

Physiochemical properties of various polymer substrates and their effects on microchip electrophoresis performance

Cited by 159 publications

References 56 publications

Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters

Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters

Study on surface properties of PDMS microfluidic chips treated with albumin

Electrozone Sensing Goes Nano

Contact Info

Product

Resources

About