Physiologic variation of sucrase activity and microbial counts in human saliva

Karjalainen, S.; Karjalainen, Mikko; Forrester, Donald J.

doi:10.1111/j.1600-0722.1992.tb01722.x

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…There is confiicting evidence in the literature about the daily variations in bacterial counts (13)(14)(15)(16)(17), in the variations from day to day (13,14,17,18) and in long-term variations (19. 20).…”

Section: Discussionmentioning

confidence: 99%

Inconsistent levels of mutans streptococci and lactobacilli measured in stimulated whole saliva

Sullivan

Hector

1995

European J Oral Sciences

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The counts of mutans streptococci (MS) and lactobacilli (LB) in saliva have been used for prediction of dental caries. The results have not justified putting the methods into clinical practice as the explanatory values of the bacteria for caries are low. There is therefore reason to question how representative the methods for sampling and estimation of those bacteria are. The aims of this study were to investigate if the variation in caries is better explained by the bacteria, estimated as means of numbers in double saliva samples, than by numbers in single samples, and to determine whether chewing on either the preferred or the non-preferred side influences the bacterial counts. Twenty-five healthy subjects were included in the study. Two saliva samples were collected with a 1-wk interval by chewing on a piece of sterile paraffin wax on the preferred side only. One month later this was repeated on the opposite side. In order to test if there were variations in the numbers of bacteria due to the laboratory methods, a paired cultivation of 20 samples was carried out. The results showed that the coefficients of variation (c.v.) for the differences between the individual double samples varied between 110 and 276% of the total mean of the double samples. The paired cultivation gave r values for MS of 0.93 and for LB of 0.99. Using double samples did not increase the explanatory values for caries. There were higher explanatory values for caries on the preferred side than on the non-preferred side, this being much more pronounced for lactobacilli than for mutans streptococci.

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Section: Discussionmentioning

confidence: 99%

Inconsistent levels of mutans streptococci and lactobacilli measured in stimulated whole saliva

Sullivan

Hector

1995

European J Oral Sciences

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“…The result was expressed in units (nmols of glucose/fructose released/min/mL saliva) of SSA. 8 A blinded examiner performed the saliva analyses. Salivary factors were analyzed and compared between groups using the Mann-Whitney test, with a significance of 5%.…”

Section: Methodsmentioning

confidence: 99%

“…High salivary sucrase activity (SSA) is considered the one that presents over 10 units of activity of sucrase. 8 From the perspective of prevention in health care, much has been said about using the tool of education as a strategy to achieve prevention. Little is discussed, however, if individuals have managed to turn the act of knowledge into everyday practices.…”

Section: Introductionmentioning

confidence: 99%

Knowledge of dental caries and salivary factors related to the disease: influence of the teaching-learning process

et al. 2015

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Knowledge of dental caries and salivary factors related to the disease: influence of the teaching-learning process Abstract: Knowledge of factors related to caries and the possible consequences in controlling the disease may show the use of education as a relevant tool for achieving success in terms of dental health maintenance. Therefore, the aim of the present study was to investigate the relationship between acquired knowledge and salivary factors related to dental caries for freshmen students (n = 44) and trainees (n = 32) of the Piracicaba Dental School. Knowledge about dental caries was evaluated by a discursive questionnaire analyzed by the content analysis technique. Salivary flow and pH, buffer capacity, salivary sucrase activity, microbiological counts (total microorganisms, mutans, and lactobacilli) and inorganic concentration of calcium, phosphorus, and fluoride in saliva were evaluated and compared between groups using the Mann-Whitney test, with a significance of 5%. Trainees demonstrated knowledge of the disease, whereas freshmen showed unspecific and confusing concepts. Among the factors analyzed, statistically significant differences were observed for pH, buffer capacity, sucrase activity, total microorganisms, and calcium and fluoride concentrations in saliva. Knowledge about the disease increases and improves over time during the undergraduate program (highest among trainees); although the students from the final year of the program consume more sugar than those from the previous one, they try to balance this activity with greater exposure to fluorides.

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“…However, diurnal variation of ms levels and variation during 3 consecutive days is limited when assessed by chair-side tests (18,19).…”

mentioning

confidence: 99%

Validation and inter‐examiner agreement of mutans streptococci levels in plaque and saliva of 10‐year‐old children using simple chair‐side tests

Karjalainen

Söderling

Pienihäkkinen

2004

Acta Odontologica Scandinavica

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Karjalainen S, So Èderling E, Pieniha È kkinen K. Validation and inter-examiner agreement of mutans streptococci levels in plaque and saliva of 10-year-old children using simple chair-side tests. Acta Odontol Scand 2004;62:153±157. Oslo. ISSN 0001-6357.Since there are few validation studies of chair-side tests of oral mutans streptococci, we compared a commercially available strip test with the conventional laboratory assay. Two plaque samples obtained from the mesial surfaces of the upper right and lower left permanent molars of sixty-five 10-year-old children (boys = 38, girls = 27) were cultured and incubated using chair-side site strip tests (Dentocult SM, Orion Diagnostica). Two plaque sampling tools, namely dental floss and micro-brush, were compared, and inter-examiner agreement between recordings of three examiners was assessed. Paraffin-stimulated saliva was then collected for laboratory and chair-side assays. The plaque and saliva chair-side tests correlated well with each other (Spearman rho, r = 0.72) and with the laboratory method, showing coefficients of 0.76 and 0.80 for saliva and plaque, respectively. Compared to the laboratory method, the sensitivity (Sn), specificity (Sp), accuracy (A), and kappa (K) values of the salivary and plaque chair-side tests were 0.63, 0.75 (Sn), 0.93, 0.90 (Sp), 0.82, 0.85 (A), and 0.58, 0.66 (K), respectively. Agreement between the two plaque sampling techniques was good (0.91). Inter-examiner agreement of plaque scores ranged between 0.65 and 0.86 when all density categories were analysed separately; when dichotomized into low and high categories, complete agreement was found. Agreement between the plaque and saliva chair-side tests and the laboratory salivary assay was good, and in terms of sensitivity, accuracy, and kappa values, the site strip plaque test surpassed the salivary chair-side test.

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Physiologic variation of sucrase activity and microbial counts in human saliva

Cited by 4 publications

References 17 publications

Inconsistent levels of mutans streptococci and lactobacilli measured in stimulated whole saliva

Inconsistent levels of mutans streptococci and lactobacilli measured in stimulated whole saliva

Knowledge of dental caries and salivary factors related to the disease: influence of the teaching-learning process

Validation and inter‐examiner agreement of mutans streptococci levels in plaque and saliva of 10‐year‐old children using simple chair‐side tests

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