Physiological and biochemical changes in pre-bearing mutants of Kinnow mandarin (C. nobilis Lour×C. deliciosa Tenora)

Mallick, Madhumita; Awasthi, O. P.; Singh, Sanjay K.; Dubey, Anil Kumar

doi:10.1016/j.scienta.2015.12.050

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…The increase in proline content in the mutants as compared to WT confirms osmo tolerance in the mutagenic population. Such findings have also been supported by Mallick et al (2016) in Kinnow mandarin and Kumar and Pandey (2019) in Coriandrum sativum.…”

Section: Biochemical Parametersmentioning

confidence: 52%

“…Facultative apomixis, self and cross-incompatibility, long juvenile period and high heterozygosis are some of the vast arrays of impediments faced by citrus breeders in conventional hybridization. Valuable mutants through chemical mutagen such as ethyl methanesulphonate (EMS) have been used as a breeding tool for creating variability and developing variety with traits of interest as it produces random point mutations in the genetic material (Predieri 2001, Dhakshanamoorthy et al 2010, Mallick et al 2016, Rime et al 2019 through the morpho-physiological and biochemical alterations. Significant alteration in EMS induced mutants have been reported to alter leaf area, chlorophyll pigments, total phenolic, proline and protein content etc.…”

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confidence: 99%

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Physiological and biochemical responses of Kinnow mandarin (Citrus nobilis × Citrus deliciosa) to EMS induced mutagenesis

et al. 2021

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The present study was carried out to enhance the genetic variability in Kinnow mandarin (Citrus nobilis Loureiro Physiological and biochemical responses of Kinnow mandarin (Citrus nobilis × Citrus deliciosa) to EMS induced mutagenesis Citrus deliciosa Tenora) using ethyl methanesulfonate (EMS) during 2016-17 and 2017-18. Plants created with different doses of EMS concentration, viz. 0.05%, 0.1%, 0.2% and 0.5% were examined for the various physiological and biochemical alterations in putative mutants aged six years and compared with the wild type (WT). A dose-dependent decrease in leaf area was recorded in the mutants with increasing doses of EMS concentration and as compared to WT, maximum reduction of 135.52 and 104.92% was witnessed in the mutants E-19 and E-17 developed from 0.5% EMS, whereas the leaves were more succulent in E-16. Chlorophyll a content was higher than chlorophyll b in the mutated population. As compared to WT, maximum decrease in chlorophyll a, total chlorophyll was noticed in the mutants E-19 generated from 0.5% EMS. Contrary to the physiological alterations, the total phenol, proline and total protein content in the mutants were upregulated by nearly two fold in the mutants developed beyond 0.1% EMS. Significant increase was also noticed in the antioxidant enzymes SOD, CAT, POX and GR activity in the mutants E-16 to E-20 induced from 0.5% EMS. The variation in traits of interest observed in the present study such as better leaf succulency and upregulated antioxidant enzyme activity in the mutant E-16 (0.5% EMS) suggests that the developed mutant could be utilised in Kinnow improvement for abiotic stress tolerance.

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Section: Biochemical Parametersmentioning

confidence: 52%

mentioning

confidence: 99%

Physiological and biochemical responses of Kinnow mandarin (Citrus nobilis × Citrus deliciosa) to EMS induced mutagenesis

et al. 2021

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“…Shoots with approximately 1 cm long were separated from the calli and were placed in MS medium without growth regulators. The survival of shoots obtained from callus without and with EMS was recorded after two months and the survival rate was calculated [13]. Shoots were placed in MS medium supplemented with indole butyric acid (IBA) for root induction.…”

Section: Shoot Regenerationmentioning

confidence: 99%

“…Ethyl methanesulfonate (EMS) is the most commonly used chemical mutagen in plantlets [12]. EMS and can cause biological effects on the function and metabolism of plant cells, which produces higher amounts of commercially useful metabolites and can therefore lead to the development of new varieties [13]. EMS, a chemical mutagen, is an alkylating agent, which has been successfully used to introduce point mutations, inducing C-to-T changes resulting in C/G to T/A substitutions [14,15].…”

Section: Introductionmentioning

confidence: 99%

Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate

Reyes-Zambrano

Ramírez-Merchant

Arias-Castro³

et al. 2019

Phyton

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A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 μM) and BAP (2.68 μM) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 μM) and BAP (44 μM). The EMS induced variations in the morphometric and morphological parameters of the plantlets obtained, with 60% of the plantlets presenting differences such as dwarfism and different leaf forms, without the presence of spines, as well as an increase in fructan content of 30% with respect to the control plantlets. PAL was increased and this activity is related with higher anthocyanins concentration in A. americana L. plantlets.

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“…All these attributes have enabled this mandarin to occupy a place of eminence owing to its major share in the area and production of citrus grown in India (Kumar et al, 2019) [5] . Despite the various positive traits, vigorous plant growth, alternate bearing, susceptibility to salinity and Phytophthora and presence of the higher number of seeds in fruits limits its productivity and scope in the processing sector (Mallick et al, 2016) [8] . Thus, seedlessness, small tree size, regular bearing, tolerant/resistant plant to various biotic and abiotic stresses are some of the main objectives of Kinnow breeding programs which however, are difficult to achieve through conventional breeding because of their high hetrozygosity, quantitative inheritance and long juvenile phase.…”

Section: Introductionmentioning

confidence: 99%

Hormonal changes in gamma irradiated and EMS induced mutants of Kinnow mandarin (Citrus nobilis Loureiro × Citrus deliciosa Tenora)

Kumar¹,

Awasthi²,

Pushkar³

2021

Int. J. Chem. Stud.

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Physiological and biochemical changes in pre-bearing mutants of Kinnow mandarin (C. nobilis Lour×C. deliciosa Tenora)

Cited by 8 publications

References 25 publications

Physiological and biochemical responses of Kinnow mandarin (Citrus nobilis × Citrus deliciosa) to EMS induced mutagenesis

Physiological and biochemical responses of Kinnow mandarin (Citrus nobilis × Citrus deliciosa) to EMS induced mutagenesis

Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate

Hormonal changes in gamma irradiated and EMS induced mutants of Kinnow mandarin (Citrus nobilis Loureiro × Citrus deliciosa Tenora)

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