Physiological and pathological implications of semicarbazide-sensitive amine oxidase

Yu, Peter H.; Wright, Shannon; Fan, Ellen H; Lun, Zhao‐Rong; Gubisne-Harberle, Diana

doi:10.1016/s1570-9639(03)00101-8

Cited by 206 publications

(170 citation statements)

References 76 publications

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“…Essential for the deamination reaction is the topa quinone (TPQ) cofactor derived from the post-translational modi®cation of a tyrosine residue in the active site (Klinman, 2003). Benzylamine is a good non-physiological substrate for human SSAO and physiological substrates are believed to include methylamine and aminoacetone (Lyles, 1996;Yu et al, 2003;O'Sullivan et al, 2004). It has been speculated that human SSAO may also use free NH 2 groups on certain proteins and aminosugars as physiological substrates Go È ktu È rk, Nilsson et al, 2003;Koskinen et al, 2004;Sibon et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Crystallization of a truncated soluble human semicarbazide-sensitive amine oxidase

et al. 2005

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Human semicarbazide-sensitive amine oxidase (SSAO) is a homodimeric copper-containing monoamine oxidase that occurs in both a membrane-bound and a soluble form. SSAO is also known as vascular adhesion protein-1 (VAP-1). A truncated soluble form of human SSAO (comprising residues 29±763) was expressed in human embryonic kidney 293 cells and puri®ed to homogeneity. Tetragonal crystals were obtained and a data set extending to 2.5 A Ê was collected. The crystals are merohedrally twinned and the estimation of the twinning fraction was complicated by pseudo-symmetry and the anisotropic character of the crystals. Using a recently developed method for twinning detection that is insensitive to phenomena such as anisotropy or pseudosymmetry [Padilla & Yeates (2003), Acta Cryst. D59, 1124±1130], the twinning fraction was estimated to be 0.3. The structure was eventually solved by molecular replacement in space group P4 3 .

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Section: Introductionmentioning

confidence: 99%

Crystallization of a truncated soluble human semicarbazide-sensitive amine oxidase

et al. 2005

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“…The decrease in K i in relation to increase in concentration of C. martini also indicates the effective inactivation of MAO at higher concentrations. The phytochemicals such as derivatives of naphthoquinone, acetylshikonin and shikonin are described as competitive inhibitors of MAO [28].…”

Section: Resultsmentioning

confidence: 99%

Kinetics of Inhibition of Monoamine Oxidase Using Cymbopogon martinii (Roxb.) Wats.: A Potential Antidepressant Herbal Ingredient with Antioxidant Activity

et al. 2011

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The study was designed to evaluate the antioxidant activity and effect of Cymbopogon martinii (Roxb.) Wats. (Poaceae) leaves on the activity of monoamine oxidase and kinetics of enzyme inhibition. Ethanol extract of C. martinii and rat brain mitochondrial monoamine oxidase preparation ware used to study the kinetics of enzyme inhibition using double reciprocal LineweaverBurk plot. The DPPH was used as a source of free radical to evaluate antioxidant potential. It is observed that, the ethanolic extract of C. martinii inhibits the monoamine oxidase activity with competitive mode of inhibition. The V max (0.01 mM/min) remained constant while, K m varied from 21.00 ± 1.1, 43.33 ± 1.5 and 83.33 ± 1.4 mM for 100-500 lg/ml concentration of C. martinii. The K i values were calculated to be 90.00 ± 0.87, 75.00 ± 0.69, 68.18 ± 0.68 lg for 100-500 lg/ml concentration of C. martini. It also shows a significant DPPH (1,1-diphenyl-2-picryl hydrazine) radical scavenging (IC 50 = 0.34 ± 0.05 mg/ml) and reducing activity (IC 50 = 0.70 ± 0.22 mg/ml). The C. martini can be considered as a possible source of MAO inhibitor used in the treatment of depression and other neurological disorders.

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“…MG synthase has been proposed to convert the triose phosphate intermediate, dihydroxyacetone phosphate (DHAP), into MG, especially when inadequate inorganic phosphate is available [50,51]. Other sources of MG, which are believed to produce lower amounts of MG, include intermediates of protein and fatty acid metabolism, such as aminoacetone produced from L-threonine and glycine [52,53], and acetone [54,55], respectively (Fig. 2).…”

Section: Methylglyoxalmentioning

confidence: 99%

“…2). Semicarbazide-sensitive amine oxidase (SSAO) catalyzes the breakdown of aminoacetone [52,55,56], while acetone and acetol mono-oxygenase (AMO) converts acetone to acetol and acetol to MG, respectively [54] (Fig. 2).…”

Section: Methylglyoxalmentioning

confidence: 99%

Aging: Drugs to Eliminate Methylglyoxal, a Reactive Glucose Metabolite, and Advanced Glycation Endproducts

Dhar¹,

Desai²

2012

Pharmacology

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Physiological and pathological implications of semicarbazide-sensitive amine oxidase

Cited by 206 publications

References 76 publications

Crystallization of a truncated soluble human semicarbazide-sensitive amine oxidase

Crystallization of a truncated soluble human semicarbazide-sensitive amine oxidase

Kinetics of Inhibition of Monoamine Oxidase Using Cymbopogon martinii (Roxb.) Wats.: A Potential Antidepressant Herbal Ingredient with Antioxidant Activity

Aging: Drugs to Eliminate Methylglyoxal, a Reactive Glucose Metabolite, and Advanced Glycation Endproducts

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