Physiological biomarkers in loggerhead turtles (Caretta caretta) as a tool for monitoring sanitary evolution in marine recovery centres

Pinya, Samuel; Renga, Emanuela; Fernández, Gloria L. Gallardo; Mateu‐Vicens, Guillem; Tejada, Silvia; Capó, Xavier; Sureda, Antoni

doi:10.1016/j.scitotenv.2020.143930

Cited by 12 publications

(5 citation statements)

References 44 publications

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“…Pinya et al [ 79 ] stated the levels of superoxide dismutase and glutathione peroxidase in the blood of sea turtles, Caretta caretta . Their SOD results in the PBMCs (peripheral blood mononuclear cells) were 1.54 (pKat/mg prot) and GPx 6.98 (nKat/mg prot), and SOD in the blood plasma was 11.8 (pKat/mg prot).…”

Section: Discussionmentioning

confidence: 99%

Biogenic Elements and Heavy Metals in Hermann’s Tortoises—Testudo hermanni: Effect on Serum Biochemistry and Oxidative Status Parameters

Kirchner,

Kirchnerová,

Tirpák

et al. 2023

Animals

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Background: Conservation of species diversity is the need of the hour for preserving life forms on Earth. Extinction of any part of the ecosystem has negative impacts on many processes and systems. The objective of this work was to analyze some biochemical and molecular indicators and their correlations to biogenic elements and heavy metals in Testudo hermanni (n = 16). Methods: Biochemical parameters were analyzed using the commercial kit DiaSys and biochemical analyzer Randox RX Monza. Sodium, potassium, and chlorides were measured using the EasyLite analyzer. Oxidative stress was evaluated using colorimetric and luminometric methods. Quantification of chemical elements in the blood was carried out using inductively coupled plasma mass spectrometry (ICPS). Results: Biochemical values of analyzed samples from Hermann’s tortoises were almost the same as referential values described by multiple authors, with minor aberrations in the total protein parameter. Values of arsenic (As) and nickel (Ni) showed correlation with biochemical parameters and the parameters of oxidative stress. Cadmium (Cd) exhibited correlation with aspartate aminotransferase (AST). Conclusions: This study reports correlations among four heavy metals, and their levels were again correlated with biochemical and molecular parameters in Hermann’s tortoises.

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Section: Discussionmentioning

confidence: 99%

Biogenic Elements and Heavy Metals in Hermann’s Tortoises—Testudo hermanni: Effect on Serum Biochemistry and Oxidative Status Parameters

Kirchner,

Kirchnerová,

Tirpák

et al. 2023

Animals

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“…The present results evidenced that the antioxidant enzyme activities—CAT, SOD, and GRd—and the levels of GSH and GST activity progressively increased in accordance with the degree of human impact. In this sense, oxidative status biomarkers are deemed to be useful to identify the effects of environmental stressors effects in marine organisms [ 66 , 67 ]. To cope with the toxic effects of MPs and potentially absorbed contaminants or other toxic compounds, organisms dispose an elaborate ROS elimination system.…”

Section: Discussionmentioning

confidence: 99%

Effects of Human Activity on Markers of Oxidative Stress in the Intestine of Holothuria tubulosa, with Special Reference to the Presence of Microplastics

Lombardo

Solomando

Cohen-Sánchez

et al. 2022

IJMS

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Pollution in the seas and oceans is a global problem, which highlights emerging pollutants and plastics, specifically microplastics (MPs), which are tiny (1 μm to 5 mm) ubiquitous plastic particles present in marine environments that can be ingested by a wide range of organisms. Holothurians are benthic organisms that feed on sediment; therefore, they can be exposed to contaminants present in the particles they ingest. The objective was to evaluate the effects of human activity on Holothuria tubulosa through the study of biomarkers. Specimens were collected in three different areas throughout the island of Eivissa, Spain: (1) a highly urbanized area, with tourist uses and a marina; (2) an urbanized area close to the mouth of a torrent; (3) an area devoid of human activity and considered clean. The results showed a higher presence of microplastics (MPs) in the sediments from the highly urbanized area in relation to the other two areas studied. Similarly, a higher number of MPs were observed in the digestive tract of H. tubulosa from the most affected area, decreasing with the degree of anthropic influence. Both in the sediment and in the holothurians, fibers predominated with more than 75% of the items. In the three areas, mesoplastics were analyzed by means of FTIR, showing that the main polymer was polypropylene (27%) followed by low-density polyethylene (17%) and polystyrene (16%). Regarding the biomarkers of oxidative stress, the intestine of H. tubulosa from the most impacted areas showed higher catalase, superoxide dismutase (SOD), glutathione reductase (GRd), and glutathione S-transferase (GST) activities and reduced glutathione (GSH) levels compared to the control area. The intermediate area only presented significant differences in GRd and GST with respect to the clean area. The activities of acetylcholinesterase and the levels and malondialdehyde presented similar values in all areas. In conclusion, human activity evaluated with the presence of MPs induced an antioxidant response in H. tubulosa, although without evidence of oxidative damage or neurotoxicity. H. tubulosa, due to its benthic animal characteristics and easy handling, can be a useful species for monitoring purposes.

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“…H 2 O 2 production was determined in algae homogenates using 2,7-dichlorofluorescein diacetate (DCFH-DA) as fluorescent probe, following a previously described method [35]. Fluorescence (Ex.…”

Section: Biochemical Analysismentioning

confidence: 99%

Oxidative Stress Response in the Seaweed Padina pavonica Associated with the Invasive Halimeda incrassata and Penicillus capitatus

Cerrato

Mir-Rosselló

Ferriol

et al. 2023

Water

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The western Mediterranean Sea is one of the most affected areas by the introduction of alien seaweed. Penicillus capitatus Lam. and the recently introduced Halimeda incrassata (J.Ellis) J.V.Lamour are tropical algae that invade native algae assemblies in the shallow sandy beds of Mallorca (Balearic Islands, western Mediterranean) where they are already settled. The aim of this study was to evaluate the effect of a potential competition between the invasive P. capitatus and H. incrassata and the native Padina pavonica (L.) Thivy, by means of biomarkers. P. pavonica samples were collected in their habitat without invasive species and areas where P. capitatus and H. incrassata cohabitated. P. pavonica densities were measured in the three investigated areas. The coexistence of the invasive algae and P. pavonica was related to a significant decrease in the densities of native algae when compared to the area without the invaders. Antioxidant enzymes, catalase and superoxide dismutase, and the reduced glutathione and polyphenols levels were significantly increased in P. pavonica in the presence of both invasive algae. Malondialdehyde, a marker of oxidative damage, and the reactive oxygen species production tended to increase in the presence of the alien species, but without significant differences. The obtained results show that the presence of P. capitatus and H. incrassata altered the normal vegetative growth of P. pavonica and caused an antioxidant response that led to a context of stress, but without evidence of oxidative damage. In conclusion, the presence of the alien H. incrassata and P. capitatus can be considered a source of competitive stress for P. pavonica, although further research regarding the increased water temperature is required.

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Physiological biomarkers in loggerhead turtles (Caretta caretta) as a tool for monitoring sanitary evolution in marine recovery centres

Cited by 12 publications

References 44 publications

Biogenic Elements and Heavy Metals in Hermann’s Tortoises—Testudo hermanni: Effect on Serum Biochemistry and Oxidative Status Parameters

Biogenic Elements and Heavy Metals in Hermann’s Tortoises—Testudo hermanni: Effect on Serum Biochemistry and Oxidative Status Parameters

Effects of Human Activity on Markers of Oxidative Stress in the Intestine of Holothuria tubulosa, with Special Reference to the Presence of Microplastics

Oxidative Stress Response in the Seaweed Padina pavonica Associated with the Invasive Halimeda incrassata and Penicillus capitatus

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