Physiological changes and transcriptional modulation of HIF-αs in Siberian sturgeon in response to hypoxia

Wang, Xiaowen; Xiao, Shusheng; Zhang, Rong; Liu, Huan; Zhu, Hua

doi:10.1016/j.aquaculture.2021.737219

Cited by 14 publications

(11 citation statements)

References 63 publications

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“…It possibly because that hypoxia exposure for some time was toxic overwhelming these antioxidant enzymes (Zhao et al, 2020). Of course, in addition to the hypoxia exposure toxic, the fluctuation of SOD, CAT and GSH‐PX activity among all the time points during hypoxia exposure in the present study is probably because of the production and elimination of oxidative substances (Liu et al, 2021; Wang, Xiao, et al, 2021). The fluctuation of antioxidant enzymes along with the increase in hypoxia time may indicate that clam could cope with temporary hypoxia by increasing antioxidant enzymes.…”

Section: Discussionmentioning

confidence: 73%

“…Four clams were selected randomly from the hypoxia and normoxia tanks at 0, 6, 12, 24, 48 and 96 h and dissected. Samples of the gills from the clam in each PVC tank were pooled to reduce individual difference, resulting in the final sample in triplicate (Wang, Xiao, et al, 2021). Gills were immediately frozen in liquid nitrogen for the antioxidant enzymes (SOD, CAT and GSH-PX) and real-time quantitative PCR (RT-PCR) analysis.…”

Section: Animals and Hypoxia Assaymentioning

confidence: 99%

“…Hypoxia‐inducible factor‐1 (HIF‐1), which has been identified as a master regulator of oxygen sensitivity, plays an important role in the adaptive responses to low oxygen availability (Piontkivska et al, 2011; Wang, Xiao, et al, 2021). Hypoxia‐inducible factor‐1 is known as a heterodimer, which contains two subunits, HIF‐1α and HIF‐1β (Heerden et al, 2004; Terova et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

Ni¹,

Wang²,

Xie³

et al. 2022

Aquaculture Research

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Aquatic hypoxia has negative or detrimental effects on clams. To cope with hypoxia stress, a complex series of physiological and biochemical strategies can be employed in clams. Hypoxia‐inducible factor‐1α (HIF‐1α) and antioxidant enzymes are commonly found in aquatic animals as an adaptation against hypoxia. In the present study, we cloned the HIF‐1α sequence in clam Cyclina sinensis, and it consists of 2654 base pairs (bp) carrying a single open‐reading frame that encompasses 2235 bp of the coding region. The predicted C. sinensis HIF‐1α protein (744 amino acids) has the conserved HLH‐PAS domain, which contained one N‐terminal basic helix–loop–helix (bHLH) domain and two Per‐ARNT‐Sim A/B (PAS) domains, followed by PAS‐associated C‐terminal (PAC) domain. HIF‐1α is expressed predominantly in gills. Exposed to hypoxia, the transcript level of HIF‐1α was significantly up‐regulated in response to hypoxia, and then, the transcript level declined continuously with the time elapsed. The antioxidant enzymes (SOD, CAT and GSH‐PX) in gills were increased initially and then decreased after acute hypoxia exposure. Our study indicates that the HIF‐1α gene and antioxidant enzymes may play an important role in cope with acute hypoxia exposure.

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Section: Discussionmentioning

confidence: 73%

Section: Animals and Hypoxia Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

Ni¹,

Wang²,

Xie³

et al. 2022

Aquaculture Research

View full text Add to dashboard Cite

show abstract

“…The hypoxia inducible factor-1 (HIF-1) belongs to a family of highly conserved transcription factors that act as main regulators of oxygen homeostasis and theadaptive response to hypoxia [ 200 , 201 , 202 , 203 ]. HIF-1 regulates the expression of many genes involved in oxygen metabolism in response to hypoxic conditions [ 200 , 202 , 204 , 205 , 206 ].…”

Section: Hypoxia Inducible Factor-1αmentioning

confidence: 99%

Role of the Neuroendocrine System of Marine Bivalves in Their Response to Hypoxia

Kotsyuba

Dyachuk

2023

IJMS

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Mollusks comprise one of the largest phylum of marine invertebrates. With their great diversity of species, various degrees of mobility, and specific behavioral strategies, they haveoccupied marine, freshwater, and terrestrial habitats and play key roles in many ecosystems. This success is explained by their exceptional ability to tolerate a wide range of environmental stresses, such as hypoxia. Most marine bivalvemollusksare exposed to frequent short-term variations in oxygen levels in their marine or estuarine habitats. This stressfactor has caused them to develop a wide variety of adaptive strategies during their evolution, enabling to mobilize rapidly a set of behavioral, physiological, biochemical, and molecular defenses that re-establishing oxygen homeostasis. The neuroendocrine system and its related signaling systems play crucial roles in the regulation of various physiological and behavioral processes in mollusks and, hence, can affect hypoxiatolerance. Little effort has been made to identify the neurotransmitters and genes involved in oxygen homeostasis regulation, and the molecular basis of the differences in the regulatory mechanisms of hypoxia resistance in hypoxia-tolerant and hypoxia-sensitive bivalve species. Here, we summarize current knowledge about the involvement of the neuroendocrine system in the hypoxia stress response, and the possible contributions of various signaling molecules to this process. We thusprovide a basis for understanding the molecular mechanisms underlying hypoxic stress in bivalves, also making comparisons with data from related studies on other species.

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“…A raise of circulating cortisol is speculated to increase energetic cost, decrease growth, increase disease susceptibility by suppressing immune function and loss of homeostasis (Davis, 2006). Physiological responses to different acute stressors have been well documented in sturgeon (Barton et al, 2000; Eslamloo & Falahatkar, 2014; Falahatkar et al, 2009; Wang et al, 2021). Since stress via cortisol plays such a major role in the regulation of stress responses, we examined the effects of DEX on the ability of sturgeon to elicit characteristic primary and secondary stress responses to an acute stressor.…”

Section: Introductionmentioning

confidence: 99%

Physiological responses to air exposure stress in Siberian sturgeon ( Acipenser baerii Brandt 1869) injected with dexamethasone

2022

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Stress can unfavorably affect fish health in hatcheries and it is important to understand how different stressors can change stress response. Dexamethasone (DEX), a synthetic glucocorticoid hormone, shows biological effects in response to stressor conditions. This study was performed to examine the effect of intraperitoneal injection of DEX on the stress response of Siberian sturgeon (Acipenser baerii), an important chondrostean species for aquaculture. For this purpose, one hundred juvenile Siberian sturgeon (average weight 279.0 ± 46.1 g [mean ±SD]) were distributed in two treatments: (1) DEX (1 mg DEX kg −1 body weight) and (2) physiological saline solution as control. 2-min air exposure was applied as an acute stressor. Blood samples were taken before injection and 0.5, 1, 2, 3, 6, 12 and 24 h post-stress. Cortisol levels showed an increasing trend in control treatment and a significant difference was found between control and DEX-treated fish. The highest levels of cortisol in DEX treatment (40.6 ± 0.4 ng mL −1 ) was recorded at 0.5 h after stress, then showed a decline and finally reached to basal level (24.2 ± 0.7 ng mL −1 ) at 24 h. The highest glucose (118.0 ± 2.9 mg dL −1 ) and lactate (125.0 ± 20.8 mg dL −1 ) values was found at 3 h post-stress in DEX-treated sturgeon and showed significant difference compared with control treatment (glucose: 84.5 ± 2.6 mg dL −1 and lactate: 56.8 ± 17.9 mg dL −1 ).After 24 h, glucose and lactate concentrations were returned to the basal level in both treatments. Our results showed that the injection of 1 mg DEX kg −1 BW to Siberian sturgeon has a potent role on stress response and describe an inhibitory effect on plasma cortisol following acute stress under laboratory condition.

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Physiological changes and transcriptional modulation of HIF-αs in Siberian sturgeon in response to hypoxia

Cited by 14 publications

References 63 publications

Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

Role of the Neuroendocrine System of Marine Bivalves in Their Response to Hypoxia

Physiological responses to air exposure stress in Siberian sturgeon ( Acipenser baerii Brandt 1869) injected with dexamethasone

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