Physiological characterization and molecular identification of some rare yeast species causing onychomycosis

Montoya, Alexandra M.; Luna-Rodríguez, Carolina E.; Bonifáz, Alexandro; Treviño-Rangel, Rogelio de J.; Rojas, Olga C.; González, Gloria M.

doi:10.1016/j.mycmed.2021.101121

Cited by 8 publications

(8 citation statements)

References 27 publications

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“…Meyerozyma was dramatically increased in type 2 short bowel syndrome rats (64). Remarkably, Meyerozyma caribbica, which was recognized as a gut fungi associated with diarrhea, has been confirmed to cause onychomycosis (65). Many fungi in the genus Rhinocladiella (e.g., Rhinocladiella mackenziei, Rhinocladiella aquaspersa, Rhinocladiella similis) are pathogens of various diseases in humans and animals (66)(67)(68).…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that Wickerhamomyces anomalus is a zoonotic pathogen that can cause severe iatrogenic infections and multiple clinical symptoms in animals (76,77). Meyerozyma caribbica is a gut fungus associated with diarrhea (65). The significant increase of Rhinocladiella similis in the diarrheal group can cause chromoblastomycosis in humans (66,78).…”

Section: Discussionmentioning

confidence: 99%

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Gut Microbial Alterations in Diarrheal Baer's Pochards (Aythya baeri)

Qin

Song

et al. 2021

Front. Vet. Sci.

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The structure and composition of gut microbiota correlate with the occurrence and development of host health and disease. Diarrhea can cause alterations in gut microbiota in animals, and the changes in the gut microbial structure and composition may affect the development of diarrhea. However, there is a scarcity of information on the effects of diarrhea on gut fungal composition and structure, particularly in Baer's pochard (Aythya baeri). The current study was performed for high-throughput sequencing of the fungal-specific internal transcribed spacer 1 (ITS-1) to detect the differences of gut mycobiota in healthy and diarrheal Baer's pochard. Results showed that the gut mycobiota not only decreased significantly in diversity but also in structure and composition. Statistical analysis between two groups revealed a significant decrease in the abundance of phylum Rozellomycota, Zoopagomycota, Mortierellomycota, and Kickxellomycota in diarrheal Baer's pochard. At the genus levels, fungal relative abundance changed significantly in 95 genera, with 56 fungal genera, such as Wickerhamomyces, Alternaria, Penicillium, Cystofilobasidium, and Filobasidium, increasing significantly in the gut of the diarrheal Baer's pochard. In conclusion, the current study revealed the discrepancy in the gut fungal diversity and community composition between the healthy and diarrheal Baer's pochard, laying the basis for elucidating the relationship between diarrhea and the gut mycobiota in Baer's pochard.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gut Microbial Alterations in Diarrheal Baer's Pochards (Aythya baeri)

Qin

Song

et al. 2021

Front. Vet. Sci.

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“…Prior reports have identified C. dermatis as a potentially infectious yeast species that has been detected in the blood, skin, and nails [ 18 , 50 ]. We identified 70 putative virulence factor genes within the C. dermatis NICC30027 genome.…”

Section: Discussionmentioning

confidence: 99%

Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

et al. 2022

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The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key economic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequencing data or associated annotations for this strain are available at present, it remains challenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic complete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mechanistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

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“…The DNA extraction of strain M503 was carried out in accordance with the instructions of the Fungal DNA Extraction Kit (Solarbio, Beijing, China). The amplification of ITS sequence with ITS primers ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-GCATATCAATAAGCGGAGGA-3′) was performed on the basis of the extracted DNA, and PCR program following the PCR protocol described by Montoya et al (2020) [ 37 ]. Sequencing was implemented by Sangon Biotech Co., Ltd., China, and BLAST was used to compare known strains and sequencing results.…”

Section: Methodsmentioning

confidence: 99%

“…The same experiments were conducted on DC and CTC. The well diffusion method was adopted to determine the antibacterial potency of TC, DC, CTC, and their degradation products [ 37 ]. Escherichia coli ATCC 25922 was selected as indicator strain and grown in LB medium at 30 °C to a steady phase.…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of Tetracycline Antibiotics by the Yeast Strain Cutaneotrichosporon dermatis M503

Tan

Kong

et al. 2022

Microorganisms

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In this study, the Cutaneotrichosporon dermatis strain M503 was isolated and could efficiently degrade tetracycline, doxycycline, and chlorotetracyline. The characteristics of tetracycline degradation were investigated under a broad range of cultural conditions. Response surface methodology (RSM) predicted that the highest degradation rate of tetracycline could be obtained under the following conditions: 39.69 °C, pH of 8.79, and inoculum dose of 4.0% (v/v, ~3.5 × 106 cells/mL in the medium). In accordance with the five identified degradation products of tetracycline, two putative degradation pathways, which included the shedding of methyl and amino groups, were proposed. Moreover, the well diffusion method showed that the strain of M503 decreases the antibacterial potency of tetracycline, doxycycline, and chlorotetracycline. These findings proposed a putative mechanism of tetracycline degradation by a fungus strain and contributed to the estimation of the fate of tetracycline in the aquatic environment.

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Physiological characterization and molecular identification of some rare yeast species causing onychomycosis

Cited by 8 publications

References 27 publications

Gut Microbial Alterations in Diarrheal Baer's Pochards (Aythya baeri)

Gut Microbial Alterations in Diarrheal Baer's Pochards (Aythya baeri)

Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

Biodegradation of Tetracycline Antibiotics by the Yeast Strain Cutaneotrichosporon dermatis M503

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