Physiological characterization of Chlamydomonas reinhardtii acclimated to chronic stress induced by Ag, Cd, Cr, Cu and Hg ions

Nowicka, Beatrycze; Pluciński, Bartosz; Kuczyńska, Paulina; Kruk, Jerzy

doi:10.1016/j.ecoenv.2016.04.010

Cited by 72 publications

(34 citation statements)

References 63 publications

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“…In addition, the leaves on the plant that received the highest Cu dose started to droop and saw increasing discoloration. Early studies indicated that phytotoxicity induced by excessive Cu uptake may lead to plant growth suppression, membrane lipid peroxidation, leaf chlorosis, and necrosis [24,37]. Some of these results and observations are in agreement with our current findings.…”

Section: Resultssupporting

confidence: 93%

RETRACTED: Morpho-Physiological and Proteomic Analyses of Eucalyptus camaldulensis as a Bioremediator in Copper-Polluted Soil in Saudi Arabia

Alotaibi

Mohammed

Almutairi

et al. 2019

Plants

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The present investigation aimed to assess the impact of copper (Cu) stress on the physiological and proteomic behavior of Eucalyptus camaldulensis. E. camaldulensis is likely a potential phytoremediator in areas vulnerable to Cu contamination, such as the industrial areas of Riyadh. To realize this objective, young seedlings of E. camaldulensis were potted in an open area with soil comprised of clay and sand. Different doses of Cu (30, 50, and 100 µM) were applied to the plants as CuSO4.5H2O for 6 weeks. Plant growth was monitored during the Cu exposure period, and morphological and physiological indicators were measured once a week to determine the growth rates. A proteomics study was also conducted to find out the influence of Cu stress on proteins. Our results showed that growth was negatively affected by Cu treatment, particularly at the highest concentrations. Moreover, using a proteomic analysis showed 26 targets involved in protein expression, which were separated by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS). Elevated levels of Cu increased the expression of 11 proteins and decreased the expression of 15 proteins. Changes were detected in proteins involved in photosynthesis, translation, transcription, metabolism, and antioxidant enzymes. Our findings provided insights into the molecular mechanisms related to Cu stress, in addition to its influence on the morphological and physiological attributes of E. camaldulensis seedlings. This investigation aimed to characterize the mechanism behind the impact of Cu stress on the plant.

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Section: Resultssupporting

confidence: 93%

RETRACTED: Morpho-Physiological and Proteomic Analyses of Eucalyptus camaldulensis as a Bioremediator in Copper-Polluted Soil in Saudi Arabia

Alotaibi

Mohammed

Almutairi

et al. 2019

Plants

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“…By disrupting protein function, Hg can inhibit electron transport and the ability of cells to generate ATP thereby inhibiting cell growth (Murthy and Mohanty 1993;Kukarskikh et al 2003;Antal et al 2009). Although toxicological studies continue to emerge evaluating the ranges of Hg concentrations tolerated by different model phototrophs (Chen et al 2014;Zhu et al 2015;Nowicka et al 2016;Mu et al 2017), very few offer new mechanistic insights (Grégoire and Poulain 2014).…”

Section: Mercury Toxicity To Phototrophsmentioning

confidence: 99%

Shining light on recent advances in microbial mercury cycling

Grégoire

Poulain

2018

FACETS

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Mercury (Hg) is a global pollutant emitted primarily as gaseous Hg0 that is deposited in aquatic and terrestrial ecosystems following its oxidation to HgII. From that point, microbes play a key role in determining Hg’s fate in the environment by participating in sequestration, oxidation, reduction, and methylation reactions. A wide diversity of chemotrophic and phototrophic microbes occupying oxic and anoxic habitats are known to participate directly in Hg cycling. Over the last few years, new findings have come to light that have greatly improved our mechanistic understanding of microbe-mediated Hg cycling pathways in the environment. In this review, we summarize recent advances in microbially mediated Hg cycling and take the opportunity to compare the relatively well-studied chemotrophic pathways to poorly understood phototrophic pathways. We present how the use of genomic and analytical tools can be used to understand Hg transformations and the physiological context of recently discovered cometabolic Hg transformations supported in anaerobes and phototrophs. Finally, we propose a conceptual framework that emphasizes the role that phototrophs play in environmental Hg redox cycling and the importance of better characterizing such pathways in the face of the environmental changes currently underway.

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“…Copper (Cu), as an essential micronutrient for plants, plays key roles in the citric acid (CA) cycle, pyruvate metabolism, and cell wall metabolism 3 , 4 . However, excess Cu induces phytotoxicity, leading to growth inhibition, stunting, leaf chlorosis, necrosis and lipid peroxidation in membrane 5 , 6 . The toxic rationales of Cu are due to its combination with nucleic acids and enzyme active sites 7 , 8 .…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions

Wang

et al. 2017

Sci Rep

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Proteomic studies were performed to identify proteins involved in the response of Oenothera glazioviana seedlings under Cu stress. Exposure of 28-d-old seedlings to 50 μM CuSO4 for 3 d led to inhibition of shoot and root growth as well as a considerable increase in the level of lipid peroxidation in the roots. Cu absorbed by O. glazioviana accumulated more easily in the root than in the shoot. Label-free proteomic analysis indicated 58 differentially abundant proteins (DAPs) of the total 3,149 proteins in the roots of O. glazioviana seedlings, of which 36 were upregulated and 22 were downregulated under Cu stress conditions. Gene Ontology analysis showed that most of the identified proteins could be annotated to signal transduction, detoxification, stress defence, carbohydrate, energy, and protein metabolism, development, and oxidoreduction. We also retrieved 13 proteins from the enriched Kyoto Encyclopaedia of Genes and Genomes and the protein-protein interaction databases related to various pathways, including the citric acid (CA) cycle. Application of exogenous CA to O. glazioviana seedlings exposed to Cu alleviated the stress symptoms. Overall, this study provided new insights into the molecular mechanisms of plant response to Cu at the protein level in relation to soil properties.

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Physiological characterization of Chlamydomonas reinhardtii acclimated to chronic stress induced by Ag, Cd, Cr, Cu and Hg ions

Cited by 72 publications

References 63 publications

RETRACTED: Morpho-Physiological and Proteomic Analyses of Eucalyptus camaldulensis as a Bioremediator in Copper-Polluted Soil in Saudi Arabia

RETRACTED: Morpho-Physiological and Proteomic Analyses of Eucalyptus camaldulensis as a Bioremediator in Copper-Polluted Soil in Saudi Arabia

Shining light on recent advances in microbial mercury cycling

Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions

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