Physiological, Chemical, Serological, and Pathogenic Analyses of a Worldwide Collection of<i>Xanthomonas campestris</i>pv.<i>vesicatoria</i>Strains

Bouzar, H.; Jones, J. B.; Stall, Robert E.; Hodge, N. C.; Minsavage, Gerald V.; Benedict, Albert A.; Alvarez, Anne M.

doi:10.1094/phyto-84-663

Cited by 106 publications

(73 citation statements)

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“…vesicatoria and the group B strains are members of X. vesicatoria. Bouzar et al (1994a) documented phenotypic diversity in a worldwide collection of strains which represented the two new species. The strains were variable in terms of carbon substrate utilization patterns, serovar and fatty acid profiles.…”

Section: Introductionmentioning

confidence: 99%

“…Strains were identified which were not typical of A-or B-group strains. Furthermore, several amylolytic strains, which were isolated from samples collected in Mexico (Bouzar et al, 1996) and the Midwest of the United States, differed from typical B strains by reacting with a panel of monoclonal antibodies like A strains (Bouzar et al, 1994a(Bouzar et al, , b, c, 1996 : these are designated group A1 in this paper. Another group of amylolytic strains was discovered (Bouzar et al, 1996 ;Jones et al, 1995), but these strains differed from the B-group strains both serologically and pathogenically and were designated group C (Bouzar et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Systematic analysis of xanthomonads (Xanthomonas spp.) associated with pepper and tomato lesions.

Jones¹,

Bouzar²,

Stall³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

131

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The taxonomy and evolutionary relationships among members of the genus Xanthomonas associated with tomato and pepper have been a matter of considerable controversy since their original description in 1921. These bacteria, which are a major affliction of tomato and pepper crops in warm and humid regions, were originally described as a single species, but subsequent research has shown the existence of at least two genetic groups differentiated by physiological, biochemical and pathological characteristics. This work synthesizes the findings from several approaches, including pathogenicity tests, enzymic activity, restriction fragment analysis of the entire genome, DNA-DNA hybridization and RNA sequence comparisons based on a 2097 base sequence comprising the 16S rRNA gene, the intergenic spacer located between the 16S and 23S rRNA genes and a small region of the 23S rRNA gene. Within the group of xanthomonads pathogenic on pepper and tomato four distinct phenotypic groups exist, of which three form distinct genomic species. These include Xanthomonas axonopodis pv. vesicatoria (A and C group), Xanthomonas vesicatoria (B group) and Xanthomonas gardneri (D group). On the basis of phenotypic and genotypic differences between A-and C-group strains, the C strains should be considered as a subspecies within Xanthomonas axonopodis pv. vesicatoria.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Systematic analysis of xanthomonads (Xanthomonas spp.) associated with pepper and tomato lesions.

Jones¹,

Bouzar²,

Stall³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

131

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“…Type A fell into DNA homology group 9, whereas type B, containing the pathovar reference strain, fell into a different DNA homology group, group 14. Both groups have been characterized by diverse genotypic (40) and phenotypic methods (6). Similarly, X. campestris pv.…”

mentioning

confidence: 99%

Reclassification of Xanthomonas

Vauterin

Hoste

Kersters

et al. 1995

International Journal of Systematic Bacteriology

868

701

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A comprehensive DNA-DNA hybridization study was performed by using 183 strains of the genus Xanthomonus. This genus was shown to comprise 20 DNA homology groups which are considered genomic species. Four groups corresponded to the previously described species Xanthomonas albilineans, Xanthomonas fiagariae, Xanthomonas oryzae, and Xanthomonas populi. The previously described species Xanthomonas campestris was heterogeneous and was divided into 16 DNA homology groups. One of these groups exhibited a high level of DNA homology with Xanthomonas axonopodis. The 62 pathovars represented in this study were. allocated to appropriate species. Our results, together with previous taxonomic data, supported a comprehensive revision of the classification of the genus Xanthomonas. The species X. albilineans, X. jiagarke, X. oryme, and X. populi are not affected. The type species of the genus,X. campestris (Pammell895) Dowson 1939, is emended to include only the pathovars obtained from crucifers (i.e., X. campestris pv. aberrans, X. campestris pv. armoraciae, X. campestris pv. barbareae, X. campestris pv. campestris, X. campestris pv. incanae, and X. campestris pv. raphani). vesicatoria. Differentiating characteristics were determined for the new species on the basis of metabolic activity on a range of carbon substrates by using the Biolog GN microplate system. X. axonopodisIn the past, the taxonomy of bacteria has been dominated by a phenetic approach, and many classification systems have been and still are based on what were thought to be important phenotypic properties. The taxonomy of the genus Xanthomonas has followed this tendency in that a single phenotypic feature, host specificity, has determined the classification of the genus. Since the first report of a xanthomonad (55) until 1974, it was common practice to define a plant-pathogenic xanthomonad isolated from a new host plant as a new Xanthomonas species. The unreasonable number of nomenspecies resulting from this practice was drastically reduced by Dye and Lelliott (19), who justified their reclassification by referring to the impossibility of differentiating nomenspecies by any feature other than host specificity (10,17). Later, names of former nomenspecies were preserved in a special-purpose classification (18) as Xanthomonas campestris pathovar names.The original classification of the genus Xanthomonas, in which all of the phytopathological variants of X campestris were recognized as separate species, was not sound taxonomically. With the exception of the ambiguous feature of host specificity, few biochemical and phenotypic characteristics were used to differentiate the species. In the last few years, * Corresponding author. Mailing address: Laboratorium voor Microbiologie, Universiteit Gent, Ledeganckstraat 35, B-9000 Ghent, Belgium.workers have provided evidence that the current classification, in whichX. campestris contains more than 140 pathovars, is not a reflection of genomic relationships. The first DNA hybridization experiments performed with Xanthomonas...

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“…Some monoclonal antibodies appear to be species-specific (Bouzar et al, 1994a). The use of serological methods in screening seeds or plant extracts would therefore require prior validation of available antibodies against the full range of pathogen diversity to be detected.…”

Section: Serological Methodsmentioning

confidence: 99%

PM 7/110 (1) Xanthomonas spp. (Xanthomonas euvesicatoria, Xanthomonas gardneri, Xanthomonas perforans, Xanthomonas vesicatoria) causing bacterial spot of tomato and sweet pepper

2013

EPPO Bulletin

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Physiological, Chemical, Serological, and Pathogenic Analyses of a Worldwide Collection ofXanthomonas campestrispv.vesicatoriaStrains

Cited by 106 publications

References 0 publications

Systematic analysis of xanthomonads (Xanthomonas spp.) associated with pepper and tomato lesions.

Systematic analysis of xanthomonads (Xanthomonas spp.) associated with pepper and tomato lesions.

Reclassification of Xanthomonas

PM 7/110 (1) Xanthomonas spp. (Xanthomonas euvesicatoria, Xanthomonas gardneri, Xanthomonas perforans, Xanthomonas vesicatoria) causing bacterial spot of tomato and sweet pepper

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