“…Physiological indicators such as heart rate [ 21 , 24 , 28 , 41 , 42 ], rectal temperature [ 21 , 28 , 41 ], respiratory rate [ 21 , 28 ], hematocrit [ 21 , 28 ], cost of energy [ 43 , 44 ], and muscle factors [ 24 , 45 ] have been studied in relation to the loading capabilities of horses and donkeys. However, the number of studies is not sufficient for detailed knowledge of the effect of load on physiological parameters of equids.…”