Physiological high temperatures alter the amino acid metabolism of bovine early antral follicles
Kohei KAWANO,
Kenichiro SAKAGUCHI,
Nattapong NINPETCH
et al.
Abstract:Heat stress reduces the developmental competence of bovine oocytes during the growth phase; however, the detailed mechanisms remain unclear. Amino acids play various critical roles in
follicular development, including protein synthesis and as energy sources. We performed
in vitro
growth (IVG) culture of oocyte–cumulus–granulosa complexes (OCGCs) to assess
the amino acid metabolism of small follicles at high temperatures. We isolated OCGCs from early antral follicles (0.5–1.0 mm) and subj… Show more
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