Physiological Involvement in pH Signaling of Vps24-mediated Recruitment of Aspergillus PalB Cysteine Protease to ESCRT-III

Rodríguez-Galán, Olga; Galindo, Antonio; Hervás-Aguilar, América; Arst, Herbert N.; Peñalva, Miguel

doi:10.1074/jbc.m808645200

Cited by 57 publications

(64 citation statements)

References 75 publications

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“…ESCRT complex function plays a role in activating the transcription factor Rim101 by recruiting the processing machinery to the endosomal membrane in a number of fungi, including C. albicans, S. cerevisiae, and Aspergillus fumigatus (10,37). In S. cerevisiae, the ESCRT-0 member Vps27 does not play a role in Rim101 activation (49), suggesting that the same may hold true in C. albicans.…”

Section: Discussionmentioning

confidence: 99%

The Candida albicans ESCRT Pathway Makes Rim101-Dependent and -Independent Contributions to Pathogenesis

et al. 2010

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Candida albicans is an opportunistic pathogen that colonizes diverse mucosal niches with distinct environmental characteristics. To adapt to these different sites, C. albicans must activate and attenuate a variety of signal transduction pathways. A mechanism of signal attenuation is through receptor endocytosis and subsequent vacuolar degradation, which requires the endosomal sorting complex required for transport (ESCRT) pathway. This pathway comprises several polyprotein complexes (ESCRT-0, -I, -II, -III, and -DS) that are sequentially recruited to the endosomal membrane. The ESCRT pathway also activates the Rim101 transcription factor, which governs expression of genes required for virulence. Here, we tested the hypothesis that the ESCRT pathway plays a Rim101-independent role(s) in pathogenesis. We generated deletion mutants in each ESCRT complex and determined that ESCRT-I, -II, and -III are required for Rim101 activation but that ESCRT-0 and ESCRT-DS are not. We found that the ESCRT-0 member Vps27 and ESCRT-DS components are required to promote epithelial cell damage and, using a murine model of oral candidiasis, found that the vps27⌬/⌬ mutant had a decreased fungal burden compared to that of the wild type. We found that a high-dose inoculum can compensate for fungal burden defects but that mice colonized with the vps27⌬/⌬ strain exhibit less morbidity than do mice infected with the wild-type strain. These results demonstrate that the ESCRT pathway has Rim101-independent functions for C. albicans virulence.

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Section: Discussionmentioning

confidence: 99%

The Candida albicans ESCRT Pathway Makes Rim101-Dependent and -Independent Contributions to Pathogenesis

et al. 2010

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“…Rim101 and the Aspergillus orthologue PacC are in vivo substrates for Cpl1 and PalB, respectively, and are activated by Cterminal processing [32,44,58,125,136]. Several other yeasts such as Candida albicans and Pichia pastoris also have Cpl1 orthologues [89].…”

Section: The Palb Subfamilymentioning

confidence: 99%

Expanding Members and Roles of the Calpain Superfamily and Their Genetically Modified Animals

2010

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Abstract:Calpains are intracellular Ca 2+ -dependent cysteine proteases (Clan CA, family C02, EC 3.4.22.17) found in almost all eukaryotes and some bacteria. Calpains display limited proteolytic activity at neutral pH, proteolysing substrates to transform and modulate their structures and activities, and are therefore called "modulator proteases". The human genome has 15 genes that encode a calpain-like protease domain, generating diverse calpain homologues that possess combinations of several functional domains such as Ca 2+ -binding domains and Zn-finger domains. The importance of the physiological roles of calpains is reflected in the fact that particular defects in calpain functionality cause a variety of deficiencies in many different organisms, including lethality, muscular dystrophies, lissencephaly, and tumorigenesis. In this review, the unique characteristics of this distinctive protease superfamily are introduced in terms of genetically modified animals, some of which are animal models of calpain deficiency diseases.

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“…Endocytic machinery is fundamental to correct PACC processing; elements of the Pal pathway interact with or are closely related to vacuolar protein sorting (VPS), and members of endosomal sorting complex required for transport (ESCRT) complexes in either Aspergillus or yeasts such as Yarrowia lipolytica and Candida albicans; in the latter, these pathways are important for growth and virulence at alkaline pH (14,35,60,126,141). In addition to PACC, tolerance to alkaline ambient pH requires the activity of two major transcriptional factors: CRZA and SLTA (138).…”

Section: Ambient Ph and Its Involvement In Intra-and Extracellular Trmentioning

confidence: 99%

Virulence Regulation of Phytopathogenic Fungi by pH

Alkan

Espeso

Prusky³

2013

Antioxidants & Redox Signaling

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Significance: Postharvest pathogens can start its attack process immediately after spores land on wounded tissue, whereas other pathogens can forcibly breach the unripe fruit cuticle and then remain quiescent for months until fruit ripens and then cause major losses. Recent Advances: Postharvest fungal pathogens activate their development by secreting organic acids or ammonia that acidify or alkalinize the host ambient surroundings. Critical Issues: These fungal pH modulations of host environment regulate an arsenal of enzymes to increase fungal pathogenicity. This arsenal includes genes and processes that compromise host defenses, contribute to intracellular signaling, produce cell wall-degrading enzymes, regulate specific transporters, induce redox protectant systems, and generate factors needed by the pathogen to effectively cope with the hostile environment found within the host. Further, evidence is accumulating that the secreted molecules (organic acids and ammonia) are multifunctional and together with effect of the ambient pH, they activate virulence factors and simultaneously hijack the plant defense response and induce program cell death to further enhance their necrotrophic attack. Future Directions: Global studies of the effect of secreted molecules on fruit pathogen interaction, will determine the importance of these molecules on quiescence release and the initiation of fungal colonization leading to fruit and vegetable losses.

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Physiological Involvement in pH Signaling of Vps24-mediated Recruitment of Aspergillus PalB Cysteine Protease to ESCRT-III

Abstract: Activation of the

Cited by 57 publications

References 75 publications

The Candida albicans ESCRT Pathway Makes Rim101-Dependent and -Independent Contributions to Pathogenesis

The Candida albicans ESCRT Pathway Makes Rim101-Dependent and -Independent Contributions to Pathogenesis

Expanding Members and Roles of the Calpain Superfamily and Their Genetically Modified Animals

Virulence Regulation of Phytopathogenic Fungi by pH

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